Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
9.2K Views
•
19:23 min
•
January 16th, 2019
DOI :
January 16th, 2019
•Chapters in this video
0:00
Title
1:05
Generation of the Substrate-coding Expression Plasmids
2:16
Expression of the Fluorescent Substrates
3:35
Cell Disruption
4:26
Ni-NTA Magnetic Agarose Bead-based Protease Assay
12:39
PAGE Analysis
14:41
Representative Results
18:29
Conclusion
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