Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
8.0K Views
•
10:50 min
•
March 14th, 2019
DOI :
March 14th, 2019
•Chapters in this video
0:04
Title
0:33
Designing the FRET Assay
1:12
Preparation of Cul1AMC•Rbx1, the FRET Donor Protein
4:42
Preparation of FlAsHCand1, the FRET Acceptor Protein
5:18
Testing and Confirmation of the FRET Assay
6:08
Measuring the Association Rate Constant
7:37
Measuring the Dissociation Rate Constant of Cul1•Cand1 in the Presence of Skp•F-box Protein
8:33
Results: Dynamics Of Protein Complexes
10:30
Conclusion
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