JoVE Table of Contents: Issue 173, July 2021

Standing Neurophysiological Assessment of Lower Extremity Muscles Post-Stroke

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Rivista (Behavior)

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

|

Rivista (Behavior)

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

|

Rivista (Biochemistry)

|

Methods Collections

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

|

Rivista (Biochemistry)

|

Methods Collections

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

|

Rivista (Biochemistry)

Preparation of High-Temperature Sample Grids for Cryo-EM

|

Rivista (Biochemistry)

|

Methods Collections

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)

|

Rivista (Biochemistry)

|

Methods Collections

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

|

Rivista (Biochemistry)

|

Methods Collections

Assessment of Submitochondrial Protein Localization in Budding Yeast <em>Saccharomyces cerevisiae</em>

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

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Rivista (Biochemistry)

|

Methods Collections

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

|

Rivista (Biochemistry)

|

Methods Collections

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

|

Rivista (Bioengineering)

From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

|

Rivista (Bioengineering)

|

Methods Collections

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

|

Rivista (Bioengineering)

|

Methods Collections

Generation and Characterization of Murine Oral Mucosal Organoid Cultures

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Rivista (Biology)

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Methods Collections

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

|

Rivista (Biology)

|

Methods Collections

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

|

Rivista (Biology)

<em>In Vivo</em> Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow

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Rivista (Biology)

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Methods Collections

Quantitative Analysis of <em>Aspergillus nidulans</em> Growth Rate using Live Microscopy and Open-Source Software

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

|

Rivista (Biology)

|

Methods Collections

Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations

|

Rivista (Biology)

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

|

Rivista (Biology)

|

Methods Collections

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

|

Rivista (Biology)

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

|

Rivista (Biology)

Probing Metabolism and Viscosity of Cancer Cells using Fluorescence Lifetime Imaging Microscopy

|

Rivista (Cancer Research)

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Methods Collections

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

|

Rivista (Chemistry)

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Methods Collections

Measuring Electrical Conductivity to Study the Formation of Brines Under Martian Conditions

|

Rivista (Chemistry)

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

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Rivista (Developmental Biology)

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Methods Collections

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of <em>Xenopus laevis</em> Embryos

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

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Rivista (Developmental Biology)

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Methods Collections

Performing <em>In Situ</em> Closed-Cell Gas Reactions in the Transmission Electron Microscope

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope

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Rivista (Engineering)

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Methods Collections

Ion-Exchange Membranes for the Fabrication of Reverse Electrodialysis Device

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Rivista (Engineering)

Field Measurement of Effective Leaf Area Index using Optical Device in Vegetation Canopy

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Rivista (Environment)

Sampling, Identification and Characterization of Microplastics Release from Polypropylene Baby Feeding Bottle during Daily Use

|

Rivista (Environment)

Application of DNA Fingerprinting using the <em>D1S80</em> Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

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Rivista (Genetics)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (<em>Oryctolagus cuniculus</em>)

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

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Rivista (Immunology and Infection)

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Methods Collections

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

|

Rivista (Immunology and Infection)

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Methods Collections

Investigating the Phagocytosis of <em>Leishmania</em> using Confocal Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

|

Rivista (Immunology and Infection)

<em>In vivo</em> Function of Differential Subsets of Cutaneous Dendritic Cells to Induce Th17 Immunity in Intradermal <em>Candida albicans</em> Infection

In vivo Function of Differential Subsets of Cutaneous Dendritic Cells to Induce Th17 Immunity in Intradermal Candida albicans Infection

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Rivista (Immunology and Infection)

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

|

Rivista (Immunology and Infection)

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of <em>Syzygium aromaticum</em> (Clove) and <em>Cuminun cyminum</em> (Cumin) on C57BL6/j Mice Fed High-Fat Diet

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet

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Rivista (Medicine)

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Methods Collections

An Endoscopic Transcanal Transpromontorial Approach for Vestibular Schwannomas using a Computer-Based Three-Dimensional Imaging System

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Rivista (Medicine)

Robot-Assisted Kidney Transplantation

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Rivista (Medicine)

|

Methods Collections

Multifactorial Assessment of Motor Behavior in Rats after Unilateral Sciatic Nerve Crush Injury

|

Rivista (Neuroscience)

Dural Stimulation and Periorbital von Frey Testing in Mice As a Preclinical Model of Headache

|

Rivista (Neuroscience)

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Methods Collections

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

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Rivista (Neuroscience)

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Methods Collections

A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution

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Rivista (Neuroscience)

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

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Rivista (Neuroscience)

Inter-Brain Synchrony in Open-Ended Collaborative Learning: An fNIRS-Hyperscanning Study

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Rivista (Neuroscience)

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Methods Collections

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

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Rivista (Neuroscience)

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Methods Collections

Measuring Engagement of Spectators of Social Digital Games

|

Rivista (Behavior)

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited <em>Helicoverpa Armigera</em> (Hübner)

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

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Rivista (Behavior)

Practical Aspects of Sample Preparation and Setup of <sup>1</sup>H <em>R<sub>1ρ</sub></em> Relaxation Dispersion Experiments of RNA

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

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Rivista (Biochemistry)

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Methods Collections

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

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Rivista (Biochemistry)

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Methods Collections

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

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Rivista (Biochemistry)

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Methods Collections

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

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Rivista (Bioengineering)

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Methods Collections

A Large Animal Model for Pulmonary Hypertension and Right Ventricular Failure: Left Pulmonary Artery Ligation and Progressive Main Pulmonary Artery Banding in Sheep

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

|

Rivista (Bioengineering)

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

|

Rivista (Bioengineering)

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

|

Rivista (Bioengineering)

Microsurgical Obstruction of Testes Fusion in <em>Spodoptera litura</em>

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

|

Rivista (Biology)

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

|

Rivista (Biology)

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

|

Rivista (Biology)

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

|

Rivista (Biology)

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [<sup>18</sup>F]FDG Micro-PET/MR Imaging

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [¹⁸F]FDG Micro-PET/MR Imaging

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Rivista (Biology)

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

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Rivista (Biology)

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Methods Collections

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional <em>In vitro</em> Model for Breast Cancer Research

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

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Rivista (Cancer Research)

Three-Dimensional <em>In Vitro</em> Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

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Rivista (Cancer Research)

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Methods Collections

A Permanent Window for Investigating Cancer Metastasis to the Lung

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Rivista (Cancer Research)

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Methods Collections

Gold Nanoparticle Synthesis

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Rivista (Chemistry)

Comparison of Two Different Synthesis Methods of Single Crystals of Superconducting Uranium Ditelluride

|

Rivista (Chemistry)

Picometer-Precision Atomic Position Tracking through Electron Microscopy

|

Rivista (Chemistry)

|

Methods Collections

Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances

|

Rivista (Chemistry)

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

|

Rivista (Developmental Biology)

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Methods Collections

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

|

Rivista (Developmental Biology)

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Methods Collections

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

|

Rivista (Developmental Biology)

|

Methods Collections

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

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Rivista (Developmental Biology)

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Methods Collections

Using Changes in Leaf Transmission to Investigate Chloroplast Movement in <em>Arabidopsis thaliana</em>

Using Changes in Leaf Transmission to Investigate Chloroplast Movement in Arabidopsis thaliana

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Rivista (Environment)

Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish

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Rivista (Environment)

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Methods Collections

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Rivista (Genetics)

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Methods Collections

Microinjection Method for <em>Anopheles gambiae</em> Embryos

Microinjection Method for Anopheles gambiae Embryos

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Rivista (Genetics)

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Methods Collections

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

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Rivista (Immunology and Infection)

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Methods Collections

Visualization of Gut Microbiota-host Interactions via Fluorescence <em>In Situ</em> Hybridization, Lectin Staining, and Imaging

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

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Rivista (Immunology and Infection)

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Methods Collections

An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury

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Rivista (Medicine)

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Methods Collections

Isolated Lung Perfusion System in the Rabbit Model

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Rivista (Medicine)

Studying Left Ventricular Reverse Remodeling by Aortic Debanding in Rodents

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Rivista (Medicine)

Accessing the Cytotoxicity and Cell Response to Biomaterials

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Rivista (Medicine)

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Methods Collections

A Preclinical Human-Derived Autologous Gastric Cancer Organoid/Immune Cell Co-Culture Model to Predict the Efficacy of Targeted Therapies

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Rivista (Medicine)

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Methods Collections

Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice

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Rivista (Medicine)

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Methods Collections

A Non-Invasive Method for Generating the Cyclic Loading-Induced Intra-Articular Cartilage Lesion Model of the Rat Knee

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Rivista (Medicine)

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Methods Collections

<em>In Vivo</em> Quantification of Hip Arthrokinematics during Dynamic Weight-bearing Activities using Dual Fluoroscopy

In Vivo Quantification of Hip Arthrokinematics during Dynamic Weight-bearing Activities using Dual Fluoroscopy

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Rivista (Medicine)

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Methods Collections

A Preclinical Model of Exertional Heat Stroke in Mice

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Rivista (Medicine)

Three-dimensional Navigation-guided, Prone, Single-position, Lateral Lumbar Interbody Fusion Technique

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Rivista (Neuroscience)

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Methods Collections

A Behavioral Screen for Heat-Induced Seizures in Mouse Models of Epilepsy

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Rivista (Neuroscience)

Shuttle Box Assay as an Associative Learning Tool for Cognitive Assessment in Learning and Memory Studies using Adult Zebrafish

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Rivista (Neuroscience)

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Methods Collections

Nuclear Transport Assays in Permeabilized Mouse Cortical Neurons

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Rivista (Neuroscience)

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Methods Collections

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery

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Rivista (Neuroscience)

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Methods Collections

Establishment of a Rat Model of Superior Sagittal-Sinus Occlusion via a Thread-Embolism Method

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Rivista (Neuroscience)

Indice

Archivio: Issue 173, July 2021

VAI A :

Standing Neurophysiological Assessment of Lower Extremity Muscles Post-Stroke

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Preparation of High-Temperature Sample Grids for Cryo-EM

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)

Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

From Cells to Cell-Free Protein Synthesis within 24 Hours Using Cell-Free Autoinduction Workflow

Formulation and Acoustic Modulation of Optically Vaporized Perfluorocarbon Nanodroplets

Generation and Characterization of Murine Oral Mucosal Organoid Cultures

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Probing Metabolism and Viscosity of Cancer Cells using Fluorescence Lifetime Imaging Microscopy

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Measuring Electrical Conductivity to Study the Formation of Brines Under Martian Conditions

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope

Ion-Exchange Membranes for the Fabrication of Reverse Electrodialysis Device

Field Measurement of Effective Leaf Area Index using Optical Device in Vegetation Canopy

Sampling, Identification and Characterization of Microplastics Release from Polypropylene Baby Feeding Bottle during Daily Use

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

In vivo Function of Differential Subsets of Cutaneous Dendritic Cells to Induce Th17 Immunity in Intradermal Candida albicans Infection

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Rapid Model to Evaluate the Anti-Obesity Potential of a Combination of Syzygium aromaticum (Clove) and Cuminun cyminum (Cumin) on C57BL6/j Mice Fed High-Fat Diet

An Endoscopic Transcanal Transpromontorial Approach for Vestibular Schwannomas using a Computer-Based Three-Dimensional Imaging System

Robot-Assisted Kidney Transplantation

Multifactorial Assessment of Motor Behavior in Rats after Unilateral Sciatic Nerve Crush Injury

Dural Stimulation and Periorbital von Frey Testing in Mice As a Preclinical Model of Headache

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

A Scalable Method to Study Neuronal Survival in Primary Neuronal Culture with Single-cell and Real-Time Resolution

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

Inter-Brain Synchrony in Open-Ended Collaborative Learning: An fNIRS-Hyperscanning Study

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Measuring Engagement of Spectators of Social Digital Games

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

A Large Animal Model for Pulmonary Hypertension and Right Ventricular Failure: Left Pulmonary Artery Ligation and Progressive Main Pulmonary Artery Banding in Sheep

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

A Permanent Window for Investigating Cancer Metastasis to the Lung

Gold Nanoparticle Synthesis

Comparison of Two Different Synthesis Methods of Single Crystals of Superconducting Uranium Ditelluride

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Functionalization of Silver/Titanium Dioxide Composites in Chitosan-based Coatings and their Egg Preservation Performances

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Use of Trowell-Type Organ Culture to Study Regulation of Dental Stem Cells

Using Changes in Leaf Transmission to Investigate Chloroplast Movement in Arabidopsis thaliana

Practical Aspects of Sample Preparation and Setup of ¹H R_1ρ Relaxation Dispersion Experiments of RNA

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [¹⁸F]FDG Micro-PET/MR Imaging