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A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

9.8K Views

07:20 min

February 28th, 2020

DOI :

10.3791/60660-v

February 28th, 2020


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3D Tumor Spheroid Model

Chapters in this video

0:04

Introduction

0:51

Melanoma Cell and Skin Fibroblast Culture

1:36

Melanoma Cell and Fibroblast Fluorescent Lentivirus Transfection

2:22

Melanoma Cell:Fibroblast Co-Culture and Melanoma Cell Solo Culture

3:25

Live Cell Time-Lapse Imaging

4:22

Confocal Microscopic 3D Movie Generation

4:59

Results: Representative 3D Tumor Spheroid Formation and Analysis

6:32

Conclusion

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