A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma
5.0K Views
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12:24 min
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September 30th, 2021
DOI :
September 30th, 2021
•Marco Y. W. Zaki*1,2, Shishir Shetty*2, Alex L. Wilkinson2, Daniel A. Patten2, Fiona Oakley*3, Helen Reeves*4,5
1Department of Biochemistry, Faculty of Pharmacy, Minia University, 2National Institute for Health Research Birmingham Liver Biomedical Research Unit and Centre for Liver and Gastrointestinal Research, Institute of Immunology and Immunotherapy, University of Birmingham, 3Newcastle Fibrosis Research Group, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, 4Newcastle University Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, 5The Liver Unit, Department of Medicine, Freeman Hospital, Newcastle-upon-Tyne Hospitals NHS Foundation Trust
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Chapters in this video
0:05
Introduction
1:20
Cell Preparation
2:17
Cell Collection and Counting
4:14
Collection of Fibroblast Conditioned Media
5:17
Heterotypic Tumor/Stromal Spheroids
6:29
Homotypic Hep3B Spheroids in LX2 Conditioned Media
8:18
Calculation of Spheroid Volume
9:17
Results: In Vitro Creation and Culture of 3D Tumor Spheroid Model
11:28
Conclusion
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