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Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

7.9K Views

09:38 min

June 26th, 2019

DOI :

10.3791/59329-v

June 26th, 2019


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HIV 1 Replication

Chapters in this video

0:04

Title

0:47

Rev-nucleolar Localization Signal (NoLS)-3'Flag Mutation Expression During HIV-1 Replication

2:03

Viral Protein Lysate Collection

3:12

Rev-NoLS-3'Flag Coimmunoprecipitation

5:20

In-gel Reduction, Alkylation, and Digestion of Coomassie-stained Gel Bands

7:14

Results: Representative Nucleolar Factor Identification During HIV-1 Replication

9:05

Conclusion

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