Chapter 16
Analyzing Gene Expression and Function
Мутагенез in vitro
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating ...
Генетический скрининг
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a ...
Тестовый кросс
Alleles are different forms of the same gene. Humans and other diploid organisms inherit two alleles of every gene, one from each parent.
An allele is ...
Тесты на комплементацию
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by ...
Однонуклеотидные полиморфизмы (SNP)
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent ...
Бактериальная трансформация
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two ...
Трансгенные организмы
Overview
Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The ...
Клонирование в целях воспроизводства
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by ...
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific ...
Экспериментальная РНК-интерференция
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. ...
Гены репортера
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually ...
Гибридизация in-situ
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled ...
Иммунопреципитация хроматина - ChIP
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or ...
Синтетическая биология
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, ...
Профилирование рибосом
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences ...
Трансгенные растения
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically ...
Генная терапия
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy ...
Анализ эпистаза
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of ...
Полногеномные ассоциативные исследования - GWAS
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more ...
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