Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
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1.8K Views
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09:37 min
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September 23rd, 2022
DOI :
September 23rd, 2022
•Linlin Zhang*1,2, Meng Zhang*1,2,3, Mengjia Liu1,2, Junhong Ai1,2, Jiao Tian1,2, Huizheng Ge4, Ran Wang1,2, Zhengde Xie1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
Rozdziały w tym wideo
0:05
Introduction
0:39
Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining
4:28
Flow Cytometry Set-Up, Gating Strategy, and Data Analysis
6:56
Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children
9:12
Conclusion
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