JoVE Logo

Zaloguj się

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

12.9K Views

06:18 min

April 3rd, 2014

DOI :

10.3791/51240-v

April 3rd, 2014


Przeglądaj więcej filmów

Keywords Brain Slice

Rozdziały w tym wideo

0:05

Title

1:17

Prepare Brain Slices

2:25

Drug Treatment, Slice Biotinylation, and Tissue Lysate Preparation

4:35

Results: Biotinylation of Brain Slices

5:55

Conclusion

Powiązane Filmy

article

09:49

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

10.3K Views

article

11:56

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

15.4K Views

article

11:32

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

16.9K Views

article

10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

15.0K Views

article

08:54

Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers

22.9K Views

article

10:42

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

9.4K Views

article

09:18

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

7.8K Views

article

07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

3.2K Views

article

10:45

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

3.3K Views

article

05:17

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

1.1K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone