Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

218 Views

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07:42 min

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February 28th, 2025

DOI :

10.3791/67745-v

February 28th, 2025

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Angela María Barbero¹^,², Rodrigo Emanuel Hernández Del Pino¹^,², Verónica Edith García³^,⁴, Virginia Pasquinelli¹^,²

¹Centro de Investigaciones Básicas y Aplicadas (CIBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), ²Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires (CIT NOBA), UNNOBA- Universidad Nacional de San Antonio de Areco (UNSAdA)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ³CONICET- Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), ⁴Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Universidad de Buenos Aires

Summary

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Fluorescence Assays

Mycobacterium Tuberculosis

Immune Receptor

SLAMF1

Biochemical Interaction

Flow Cytometry

Fluorescence Microscopy

Macrophage Microbial Sensor

Total Cell Protein Extracts

Antigen Interaction

Cross linking Treatment

Rhodamine PE

Poly D lysine

Monocyte derived Macrophages

Immunoreceptor Interactions

Chapters in this video

0:00

Introduction

2:11

Monocyte-Derived Macrophage Culture, Stimulation, and Protein Extraction

4:42

Investigating SLAMF1- Mycobacterium tuberculosis Interaction by Flow Cytometry

5:32

Investigating SLAMF1- Mycobacterium tuberculosis Interaction by Fluorescence Microscopy

6:42

Representative Results

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