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High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures

2.7K Views

09:36 min

March 10th, 2021

DOI :

10.3791/61988-v

March 10th, 2021


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Neural Stem Cells

Chapters in this video

0:04

Introduction

0:48

Fetal Neural Stem Cell (NSC) Dissection and Isolation

2:18

Adult NSC Culture

4:05

Primary Neurosphere, Oligosphere, and Oligodendrocyte Precursor Cell (OPC) Differentiation

5:05

Inflammation-Mediated Differentiation Block and Oxygen-Glucose Deprivation (OGD) Cell Death Induction

5:56

High-Content Screening (HCS) Cell Viability, Lineage Composition, and Lineage-Specific Cell Death Analyses

7:18

Results: Representative Oligosphere Differentiation Characterization

9:04

Conclusion

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