Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
11.8K Views
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11:11 min
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February 17th, 2016
DOI :
February 17th, 2016
•Chapters in this video
0:05
Title
0:50
Rat Brain Dissection
4:20
Enzymatic and Mechanical Tissue Dissociation
5:26
Anti-A2B5 Bead Application, Column Purification, and Plating
6:29
Induction of OPC Differentiation and Fixation with 4% Paraformaldehyde
7:38
Immunocytochemistry and Qualification of Myelin Basic Protein
9:25
Results: OPC Culture and Differentiation Procedure
10:29
Conclusion
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