Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
7.4K Views
•
10:18 min
•
June 13th, 2019
DOI :
June 13th, 2019
•Vinciane Saint-Criq1, Iram J. Haq2,3, Aaron I. Gardner2, James P. Garnett2,4, Christopher Ward1,2, Malcolm Brodlie2,3, Michael A. Gray1
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, 3Paediatric Respiratory Medicine, Great North Children's Hospital, Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co
Chapters in this video
0:04
Title
1:09
Primary Human Airway Epithelial Cell (hAEC) Preparation
1:53
Background Measurement
4:43
Kinetics Measurement
5:45
In Situ pH Calibration
6:28
Data Analysis
7:58
Results: Representative pH Calibration in Primary hAECs In Situ
9:34
Conclusion
Related Videos
12:09
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
22.3K Views
08:04
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
14.1K Views
10:46
Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
3.9K Views
05:31
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
10.2K Views
06:27
iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease
719 Views
07:38
Preparation and Structural Evaluation of Epithelial Cell Monolayers in a Physiologically Sized Microfluidic Culture Device
1.2K Views
10:26
P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
10.7K Views
09:02
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
1.1K Views
11:13
Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency
3.8K Views
07:40
Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
416 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved