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Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

9.7K Views

10:59 min

May 12th, 2019

DOI :

10.3791/59478-v

May 12th, 2019


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Human Induced Pluripotent Stem Cells

Chapters in this video

0:04

Title

0:38

Differentiation from iPSCs to the Hindgut

1:10

Embedding of the Hindgut in Basement Membrane Matrix

3:13

Maintenance, Passage, and Pre-stimulation of iHOs with rhIL-22

5:28

Microinjection of iHOs and Intracellular Invasion Assays

9:19

Results: Studying Epithelial Cell Protection Against Pathogens Using hiPSC-derived iHOs

10:05

Conclusion

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