Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
2.9K Views
•
07:59 min
•
September 10th, 2021
DOI :
September 10th, 2021
•1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)
Chapters in this video
0:04
Introduction
1:04
Preparation of Single-Cell Solution and Preparation of Gel Beads-in-Emulsion Under Biosafety Level 3 Conditions
5:47
Results: Characterization of Host/Pathogen Interactions at a Single-Cell Level Using scRNAseq Technology
7:11
Conclusion
Related Videos
10:30
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
35.1K Views
10:59
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
9.7K Views
10:56
Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
13.4K Views
07:18
Investigating Intestinal Barrier Breakdown in Living Organoids
10.9K Views
09:18
A Video Protocol of Retroviral Infection in Primary Intestinal Organoid Culture
29.6K Views
09:11
In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis
2.1K Views
06:51
Microfluidic Model of Necrotizing Enterocolitis Incorporating Human Neonatal Intestinal Enteroids and a Dysbiotic Microbiome
1.1K Views
07:49
Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models
10.6K Views
Generation and Characterization of Murine Oral Mucosal Organoid Cultures
2.0K Views
08:23
Using Human Intestinal Organoids to Understand the Small Intestine Epithelium at the Single Cell Transcriptional Level
664 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved