Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
58.8K Views
•
08:40 min
•
March 1st, 2019
DOI :
March 1st, 2019
•Jean K. Millet1,2, Tiffany Tang3, Lakshmi Nathan3, Javier A. Jaimes4, Hung-Lun Hsu3,5, Susan Daniel3, Gary R. Whittaker1
1Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, 5Horae Gene Therapy Center, University of Massachusetts Medical School
Chapters in this video
0:04
Title
0:41
Cell Seeding for Pseudotyped Particle Production
2:05
Three-plasmid Co-transfection
3:53
Pseudotyped Particles Collection
4:46
Pseudotyped Particle Infection
5:54
Infectivity Quantification by Luciferase Assay Readout
7:04
Results: Infectivity Assays of SARS-CoV S and MERS-CoV S Pseudotyped Particles
8:02
Conclusion
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