Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics

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549 Views

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08:50 min

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August 16th, 2024

DOI :

10.3791/67227-v

August 16th, 2024

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Frederic St-Denis-Bissonnette*¹^,², Melanie Kirkby*¹^,², Lisheng Wang²^,³^,⁴, Jessie R. Lavoie¹^,²

¹Biologic and Radiopharmaceutical Drugs Directorate, ²Department of Biochemistry, Microbiology and Immunology Institute, University of Ottawa, ³Centre for Infection, Immunity and Inflammation Institute, University of Ottawa, ⁴Regenerative Medicine Program, Ottawa Hospital Research Institute

Podsumowanie

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Biomanufacturing Workflow

Natural Killer Cells

Extracellular Vesicles

Cancer Biotherapeutics

Size Exclusion Chromatography

Vesicle Isolation

Therapeutic Potential

Protein Liquid Chromatography

EV rich Conditioned Media

Endonuclease Treatment

Preclinical Models

Chromatography System

Rozdziały w tym wideo

0:00

Introduction

1:14

Purification and Sterilization of Natural Killer Cell Derived-Extracellular Vesicles (NK-EVs) Using Fast Protein Liquid Chromatography and Ultrafiltration for Nanoparticles Tracking Analysis

3:57

Filter-Based Sterilization and Concentration of NK-EV Product

5:16

Characterization of Natural Killer Cell Derived-Extracellular Vesicles (NK-EVs) Using Nanoparticle Tracking Analysis

7:53

Representative Results

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