A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Please note that all translations are automatically generated. Click here for the English version.

7.2K Views

•

10:44 min

•

August 9th, 2019

DOI :

10.3791/58672-v

August 9th, 2019

•

Raul Vizcardo*¹^,², S.M. Rafiqul Islam*¹^,², Takuya Maeda¹^,², Naritaka Tamaoki¹^,², Meghan L. Good¹^,², Nicholas D. Klemen¹^,², Marta Bosch-Marce¹^,², Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo¹^,²

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

Podsumowanie

Przeglądaj więcej filmów

3D Thymic Organ Culture

IPSC derived Thymic Emigrants

Tumor Antigen specific T Cells

Naive T Cell like Phenotype

CD8 Alpha beta T Cells

Cell Culture Procedure

Cell Suspension Preparation

Tumor Suppression

Proliferation Capacity

Experimental Methodology

Rozdziały w tym wideo

0:04

Title

0:57

Preparation of OP9/DLL1 Cells for Co-culture with iPSC

2:08

In Vitro Differentiation of iPSC into Immature T Cells

5:00

3D Thymic Organ Culture to Generate iTE

8:00

Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

9:46

Conclusion

Powiązane Filmy

article

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

10.7K Views

article

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

8.2K Views

article

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

9.8K Views

article

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

18.5K Views

article

Reaggregate Thymus Cultures

12.9K Views

article

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

10.3K Views

article

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8⁺ T Cells in Mice

6.4K Views

article

Formation of Human Thymus Organoids in Three-Dimensional Fibrin Hydrogels

1.4K Views

article

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

6.6K Views

article

Trójwymiarowy system hodowli grasicy do generowania emigrantów grasicy pochodzących od iPSC

88 Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone