CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Please note that all translations are automatically generated. Click here for the English version.
391 Views
•
08:23 min
•
November 1st, 2024
DOI :
November 1st, 2024
•1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, 2Gates Institute, University of Colorado School of Medicine, Anschutz Medical Campus, 3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Anschutz Medical Campus
Rozdziały w tym wideo
0:00
Introduction
1:23
Cell Culture and DNA Shearing Protocol for Genomic DNA Preparation from Induced Pluripotent Stem Cells
3:28
CIRCLE-Seq Library Preparation for Genomic DNA Circularization and Purification
5:58
In Vitro Cleavage of Circularized Genomic DNA and CIRCLE-Seq Data Analysis
Powiązane Filmy
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
16.2K Views
09:37
Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
13.0K Views
10:07
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
7.7K Views
16:08
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
14.8K Views
06:11
Production and Purification of Baculovirus for Gene Therapy Application
14.6K Views
09:25
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
9.8K Views
09:56
CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
11.7K Views
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
21.3K Views
11:37
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
9.6K Views
08:31
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
13.8K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone