Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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9.6K Views
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11:37 min
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February 26th, 2019
DOI :
February 26th, 2019
•Rozdziały w tym wideo
0:04
Title
1:45
Positive and Negative Screening for Evolving Cas9
4:47
Delivery of Cas9 as an RNP with Truncated sgRNA
6:17
RNP Delivery
7:27
Transfection of Plasmids Encoding Sniper-Cas9 and sgRNA
9:01
Targeted Deep Sequencing for the Analysis of On-target and Potential Off-target Sites
9:29
Results: Analysis of Sniper-screen, On-target and Off-target Activities
10:37
Conclusion
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