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Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images

2.7K Views

12:45 min

August 31st, 2022

DOI :

10.3791/63992-v

August 31st, 2022


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Tunneling Nanotubes

Rozdziały w tym wideo

0:05

Introduction

1:28

Immunostaining of F-Actin and Activated-PAK1 for the Characterization of Tunneling Nanotubes

4:16

Imaging with Confocal Microscopy

6:00

Analysis of Confocal Z-Stack Images to Identify and Quantify Tunneling Nanotubes

9:45

3D Reconstruction of Z-Stack Images to Characterize Tunneling Nanotubes

11:00

Results: Characterization of Tunneling Nanotubes by Constructing a 3D Volume View of Confocal Z Stack Images

12:11

Conclusion

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