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Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins

12.0K Views

10:03 min

August 10th, 2014

DOI :

10.3791/51310-v

August 10th, 2014


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Keywords Nuclear Enriched Fraction

Rozdziały w tym wideo

0:05

Title

2:03

Preparation of Hippocampal Slices

3:13

Baseline Recording and LTP Induction

5:17

Isolation of Nuclear Enriched Fractions from Hippocampal CA1

7:29

Semiquantitative Immunoblotting for Synapto-nuclear Proteins

8:02

Results: Induction of CA1 LTP Leads to the Accumulation of pJacS180 in the Nucleus

8:53

Conclusion

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