JoVE Logo

Zaloguj się

Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain

5.7K Views

12:14 min

September 14th, 2022

DOI :

10.3791/64574-v

September 14th, 2022


Przeglądaj więcej filmów

Subcellular Fractionation

Rozdziały w tym wideo

0:04

Introduction

1:37

Mouse Brain Excision

3:32

Synaptosome Preparation

5:23

Hypotonic Lysis

6:24

Synaptic Vesicle Isolation

7:35

Synaptic Plasma Membrane Isolation

10:02

Results: Electron Microscopy of Synaptosomes and Immunoblot Analysis of Subcellular Fractions

11:00

Conclusion

Powiązane Filmy

article

09:49

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

10.3K Views

article

08:30

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

31.4K Views

article

08:06

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

31.1K Views

article

10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

10.4K Views

article

09:07

Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins

11.9K Views

article

09:18

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

7.8K Views

article

09:49

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

10.7K Views

article

07:14

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

3.6K Views

article

07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

1.6K Views

article

01:52

Izolacja komórek jednojądrzastych z mózgu myszy: technika wirowania w gradimencie gęstości do izolowania komórek jednojądrzastych rezydujących w mózgu

1.2K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone