We would like to acknowledge the funding contributions of the Canadian Surgical Research Fund, the Edmonton Civic Employees Research Assistance Fund, and the Alberta IBD Consortium through a grant from Alberta Innovates.

동물 사용 프로토콜은 알버타 대학의 건강 과학 동물 관리 및 사용위원회에 의해 승인되었습니다. 악기, 동물, 수술 설치 1. 준비 <ol><li> 절차 이전에 모든 고체 음식 24 시간의 새로운, 깨끗한 케이지 결석에 동물을 전송합니다. 그들은 절차의 시간 동안 물에 자유롭게 접근하고, 액체 다이어트 적량을 가질 수있다. </li><li> 절차에 필요한 모든 악기를 압력솥. 클린 운영면과 70 % 에탄올로 마취 코 콘. </li><li> 운영 외과 의사를위한 편안한 방식으로 운영 현미경, 마취 기계 및 소모품과 운영면을 설정합니다. 외과 의사의 팔꿈치는 손과 장비에 의해 방해받지 팔, 운영 테이블에 편안하게 휴식을 허용해야합니다. 악기, 봉합, 면봉, 그리고 10 ML의 주사기는 절차를 수행하는 동안 쉽게 액세스를 허용하는 위치에 배치해야합니다. </li><li> 설정열 헤드 램프는 조작면에 대한 절차와 빛이 동안 동물 용 온기를 모두 제공한다. </li><li> 0.9 % 식염수 50 ML 원뿔 튜브 및 운영 표면 근처 석유 젤리 및 장소 1.5 ML 튜브를 입력합니다. 참고 : 모든 악기와 수술 용품을 소독. 소장이 가로로되어 있기 때문에, 절차 자체 멸균 아니다. 그것은 깨끗하고 오염 된 것으로 간주됩니다. 감염의 외생 소스의 도입을 방지 할 수있는 조치를 취하십시오. </li></ol> 문합 2. Ileocolic 절제술 <ol><li> 동물의 호흡 속도가 약 30 내지 40 호흡 / 분까지 저하 이소 플루 란 기화기로부터 노즈콘을 통해 / 분 2 L의 산소 유량을 갖는 4 % 이소 플루 란을 투여하여 마취를 유도한다. 절차를 시작하기 전에 고통의 응답이없는 보장하기 위해 마우스의 뒷발에 적당한 압력을 적용합니다. 이 시점에서, / 분으로 0.5 L로 2 % 이소 플루 란과 산소 흐름을 거절. 간헐적으로절차 동안 통증 반응을 확인하고 그에 따라 이소 플루 유량을 조정한다. </li><li> 수술 중 건조를 방지하고, 투명 테이프를 사용하여 보호 사지와 부정사 위치에 마우스를 고정하기 위해 눈에 바셀린을 적용합니다. </li><li> povodine / 요오드 용액으로 복부를 청소하고, 새로운 멸균 장갑으로 변경합니다. </li><li> 근막과 복막을 노출 가위를 해부 날카로운 포인트를 사용하여 복부의 상단 중간 선에서 1.5 cm의 피부 절개를합니다. 복막 내용을 노출하는 원격 교육의 알바를 통해 비슷한 방식으로 근막 / 복막 계층을 엽니 다. </li><li> 인간 대조적으로, 마우스는 일반적으로 맹장 복부 좌상 사분면에 발견된다. 확인되면, 부드럽게 집게로 맹장을 잡고 절개를 통해 제공합니다. 팬에 적신 면봉을 사용하여 밖으로 약 복부 표면 (그림 1A)에 드리 워진 멸균 거즈를 통해 맹장에서 연장 회장 말단의 3cm.노출 된 창자가 절차의 전체 중 0.9 % 식염수와 습기가 유지되어 있는지 확인합니다. </li><li> 운영 현미경을 사용하여 콜론 (그림 1A)를 따라 상 장간막 동맥 떨어져 분기 회맹 동맥을 확인합니다. , 회맹 동맥에 인접한 무 혈관 조직을 해부 둘러싸고 5-0 실크 넥타이와 동맥을 결찰. 다음으로, 회장 말단에 지역 혈액 공급을 찾아 회맹 접합에 절개 포인트 1.5 형상 관리 근위을 선택합니다. 위와 회장의이 부분에 가지를 결찰. 마이크로 가위를 해부와 동맥을 나눈다. </li><li> 가로로 끝 (그림 1B)에 충분한 혈액 공급이 보장 회장과 대장의 ​​허혈성 부분을 나눈다. 그것은 더 밀접하게 일치하도록 결장 내강의 직경을 증가시키기 위해 30도 각도로 분할하여 회장을 주걱 것이 종종 도움이된다. 창자 회맹 부분이 제거 된 후, 정렬각각의 장간막 국경을 보장 거즈에 회장과 대장의 가로로 끝이 정렬됩니다. </li><li> 사용 대장의 가로로 마지막에 회장의 가로로 끝을 근사하여 문합 구축은 8-0 폴리 프로필렌 봉합사 (그림 1C)를 중단. Ileocolic 문합은 방수가 될 때까지 이후의 봉합은 모든 0.5 mm를 배치와 함께 첫 번째 스티치, 장간막 국경에 배치됩니다. 회장과 대장을 통해 봉합 바늘을 통과하는 경우, 절단 된 모서리가 롤백되지 않도록하고, 바늘 물린 대장의 절단면에서 0.5 mm입니다. 전형적인 문합은 14-16 중단 봉합이 필요합니다. 문합을 통해 내용을 강제로 회장을 통해 원위 면봉 근위 롤링에 의해 완료시 문 합부의 무결성과 개통을 테스트합니다. 소장의 내용은 자유롭게 문 합부 누출없이 대장에 전달해야합니다. </li><li> 10 ML의 주사기에서 0.9 % 식염수 3-4 mL를 노출 창자를 씻어대장의 표면에서 대변을 씻어하고, 복강에 다시 장을 제공합니다. 0.9 % 식염수 2 ㎖를 사용하여 복강 씻은 다음 횡 복벽에 약간 힘을인가하여 유체를 배수. </li><li> 3-0 실크 실행 봉합과 절개를 닫고, 이소 플루 란의 흐름을 중단. 수술 후 통증 조절을 위해 -buprenorphine- 피하 긴 작용하는 아편의 0.1 ㎎ / ㎏을 관리합니다. </li><li> 그들은 다음 이동성 지속적으로 따뜻하게 케이지로 전송 될 때까지 가열 램프 아래에있는 동물을 관찰한다. </li></ol> 3. 수술 후 관리 및 모니터링 <ol><li> 하루의 나머지 부분에 대한 고민의 흔적이 지속적으로 따뜻하게 새장에 동물을 모니터링합니다. 액체 다이어트와 물 적량에 액세스 할 수있는 새로운 멸균 케이지 다시 동물 보호 시설에 동물을 전송합니다. 동물은 3-4 그룹에 수납 할 수있다. </li><li> 다음날 아침 수술 후 동물 검사를 수행,동물이 고통에 표시되지 않도록. 만 액체 음식을 공급. 그들이 불편 (즉, 웅크 자세 또는 최소한의 활동을) 표시되면 피하 부 프레 노르 핀의 추가 용량을 관리 할 수 있습니다. 수술 후 1 일에 오후에 한 번 더 동물에 확인합니다. </li><li> 수술 후 2 일째의 아침에 동물이 완전히 회복 나타납니다. 음식 소비와 stooling의 증거는 회복의 긍정적 인 신호이다. 자, 동물 용 고체 차우 다이어트를 다시 시작합니다. 참고 : 고통의 징후가 웅크 자세, 가난한 정리, 최소한의 활동을 포함한다. 고통의 흔적이 눈에 띄는 경우 동물은 안락사되어야한다. </li><li> 동물까지 2 L / 분의 O 2 유량 4 % 이소 플루 란 깊은 마취를 유도함으로써 동물을 안락사는 족압에 응답한다. 경추 탈구를 수행하고 적절한 euthanization의 흔적을 관찰합니다. </li><li> 가이드 라인은, 그래서 표시에 관한 기관의 권고 사항을 참조 달라및 마우스에 euthanization의 적절한 방법. </li></ol>

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The authors have nothing to disclose

뮤린 ICR는 장 질환 수술의 효과를 연구하기 위해 사용될 수있는 강력한 모델이다. 이 기사는 95 %의 성공률과 업 시술 후 이십팔일 안정 가중치 반영된 번창 부전 문제없이 ICR 마우스에서 수행 방법을 설명한다. 성공적인 ICR에 가장 중요한 과제는 문합과 문 합부 누출에 장 장애물을 피하는 등이 있습니다. 폐색을 방지하기위한 수술 기술 요소는 문합에 내강 직경을 최대화위?...

Ileocolic resection (ICR) is a common procedure performed in both emergent and elective situations for a variety of illnesses. Crohn&#8217;s disease and colon cancers are the two most common indications for ICR. In both illnesses, recurrence in the bowel at the site of surgery represents a major clinical problem. Local recurrence rates for colon cancer remain an issue even with the most aggressive resections1. Following ICR in Crohn&#8217;s disease, the disease most frequently (in up to 80%) recurs in the neo-terminal ileum at 1 year after surgery2. Given the impact of these two illnesses and their recurrence after surgery, it is important to understand local intestinal factors after ICR, which may have intrinsic influences on the natural history of these diseases. Further, it is also important to consider anastomotic healing after ICR. In the early post-operative period, anastomotic leaks can have devastating consequences for patients resulting in repeat surgeries, stoma creations, significant morbidity, and even mortality3. Despite the importance of this topic, our current understanding of anastomotic healing remains in its infancy as a subject of research. Animal models of ICR, in particular mice, are an excellent platform for studying the intestinal and anastomotic healing following surgery.
A mouse model of ICR was initially developed by Helmrath et al. to be used as a model of short gut syndrome4. The authors experimented with various diet regiments and suture sizes to optimize animal survival following ICR. They concluded that feeding with liquid diet in the perioperative period and using 9-0 monofilament sutures resulted in an optimal post-operative survival of 88%. Since this initial publication, ICR in mice removing 50% of the small bowel has been used in several studies to explore the dynamics of massive small bowel resection and the adaptive growth response in attempt to develop new therapies for short gut syndrome5,6.
The first application of the ICR mouse model to Crohn&#8217;s disease used the IL-10-/- mouse model, which spontaneously develops colitis7. The authors found that after ICR these animals developed inflammation in the neo-terminal ileum similar to that seen in post-operative Crohn&#8217;s disease patients, and that this inflammation was dependent on the presence of bacteria7. More recently, this model was used to explore bacterial changes induced by ICR. In Crohn&#8217;s disease there is an associated dysbiosis with relative decreases in bacteria known to have anti-inflammatory properties and increases in invasive species of bacteria8,9. The association holds true in cases of post-operative recurrence10. Two studies sought to identify microbial changes resulting from ICR. The first used IL-10 null mice, and performed denaturing gel electrophoresis to compare bacterial similarity between the small bowel and colon after ICR11. This study demonstrated that bacterial populations became similar in the small intestine and colon following ICR. A subsequent study used wild type mice and 16s pyrosequencing for phylogenetic classification of bacterial species post-operatively. This study demonstrated a marked shift in bacterial species resulting from surgery alone with Clostridium species becoming dominant as well as an increase in &#978;-proteobacteria. The results also confirmed the findings of the previous study with similar populations found in the small bowel and colon after ICR12. 
ICR is a common procedure for patients with colon cancer involving the cecum and ascending colon, and it is becoming increasingly recognized that the host-response to surgery likely contributes to both local and distant tumor recurrence13. Despite this observation, models of ICR have not been utilized for the study of colorectal cancer and post-operative recurrence. Understanding both the systemic and local immunologic changes resulting from ICR will be important in investigating future therapies. Potential pathways involved in cancer recurrence post ICR include up regulation of growth factors, which may rescue cells from apoptosis and stimulate proliferation, mechanical tumor disruption with cell shedding, and loss of immune surveillance through post-operative immunosuppression13,14. 
Mouse models of ICR have the potential to be a powerful tool for the investigation of short bowel syndrome, Crohn&#8217;s disease, and colon cancer. They may also provide lessons on how to prevent early post-operative anastomotic complications by further defining the cellular and biochemical pathways involved in healing the newly constructed anastomosis. A major barrier in utilizing the murine ICR model is the technical difficulty. The intestinal anastomosis requires the use of 8-0 or 9-0 suture, an operating microscope, and training in microsurgical techniques. The goal of this article is to provide clear instructions on how to perform ICR in mice with the goal of utilizing this procedure in models of disease.

<table><tbody><tr><td>LD101 liquid rodent diet</td><td>testdiet.com</td><td></td><td></td></tr><tr><td>0.9% NaCl</td><td>Baxter</td><td>FKE1324</td><td>Injection quality saline</td></tr><tr><td>Operating Microscope</td><td>Ziess</td><td></td><td></td></tr><tr><td>Isoflurane Anesthetic Vaporizer</td><td>Harvard Apparatus</td><td>34-0483</td><td></td></tr><tr><td>Isoflurane</td><td>Abbott&amp;nbsp;</td><td>05260-05</td><td></td></tr><tr><td>Glass plate</td><td></td><td></td><td>For operating surface</td></tr><tr><td>Cotton swabs</td><td></td><td></td><td></td></tr><tr><td>Micro Castroviejo Needle holder, curved</td><td>World Precision Instruments</td><td>503377</td><td></td></tr><tr><td>Castroviejo straight scissors</td><td>World Precision Instruments</td><td>555530S</td><td></td></tr><tr><td>Dissecting Scissors</td><td>World Precision Instruments</td><td>15922</td><td></td></tr><tr><td>Dressing Forceps x 2</td><td>World Precision Instruments</td><td>500363</td><td></td></tr><tr><td>5-0 silk pre-cut sutures</td><td>Ethicon</td><td>A182H</td><td>For vessel ligation</td></tr><tr><td>8-0 Prolene on BV130-5 needle</td><td>Ethicon</td><td>8732H</td><td>For anastomosis</td></tr><tr><td>3-0 Silk on FS-2 needls</td><td>Ethicon</td><td>8665G</td><td>For abdominal wall closure</td></tr><tr><td>Petroleum Jelly</td><td>Vaseline</td><td></td><td></td></tr><tr><td>10 ml syringe</td><td>BD biosciences</td><td></td><td></td></tr><tr><td>Povidone-iodine 7.5% surgical Scrub</td><td>&lt;a href=&quot;http://betadine.com&quot;&gt;betadine.com&lt;/a&gt;</td><td></td><td></td></tr><tr><td>Heat lamps</td><td></td><td></td><td></td></tr><tr><td>buprenorphine 0.3 mg/ml</td><td>Reckitt Benckiser Healthcare Ltd.&amp;nbsp;</td><td>PL36699/0006</td><td></td></tr></tbody></table>

murine ileocolic bowel resection with primary anastomosis

Intestinal resections are frequently required for treatment of diseases involving the gastrointestinal tract, with Crohn&#8217;s disease and colon cancer being two common examples. Despite the frequency of these procedures, a significant knowledge gap remains in describing the inherent effects of intestinal resection on host physiology and disease pathophysiology. This article provides detailed instructions for an ileocolic resection with primary end-to-end anastomosis in mice, as well as essential aspects of peri-operative care to maximize post-operative success. When followed closely, this procedure yields a 95% long-term survival rate, no failure to thrive, and minimizes post-operative complications of bowel obstruction and anastomotic leak. The technical challenges of performing the procedure in mice are a barrier to its wide spread use in research. The skills described in this article can be acquired without previous surgical experience. Once mastered, the murine ileocolic resection procedure will provide a reproducible tool for studying the effects of intestinal resection in models of human disease.

사망률과 체중 변화 수술 후. 129S1 야생형 생쥐에서 ICR을 다음과 사망률 ~ 5 % 일반적이다. 도덕의 가장 일반적인 원인은 문합에서 장폐색입니다. 사망의 다른 원인은 문 합부 누출과 폐쇄를 창자로 이어지는 내부 탈장을 포함한다. 체중 감소는 수술 후 최대 14 일까지 볼 수 있지만, 일반적으로 비 중요하다 할 수있다. 마우스는 완전히 수술 후 28 일째 (그림 2)...

Watch this Scientific Journal Video about Murine Ileocolic Bowel Resection with Primary Anastomosis at JoVE.com

Murine Ileocolic Bowel Resection with Primary Anastomosis

Ileocolic resection is commonly performed in several human diseases; however, little is known regarding the impact of intestinal resection on surgical illnesses. This article provides instruction on executing the procedure in mice with high success, providing a means to study the effects of ileocolic resection in models of disease.

차 문합와 쥐의 Ileocolic 대장 절제술

Intestinal resections are frequently required for treatment of diseases involving the gastrointestinal tract, with Crohn&#8217;s disease and colon cancer ...

murine-ileocolic-bowel-resection-with-primary-anastomosis

Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

16.3K 조회수.  University of Alberta. Ileocolic resection is commonly performed in several human diseases; however, little is known regarding the impact of intestinal resection on surgical illnesses. This article provides instruction on executing the procedure in mice with high success, providing a means to study the effects of ileocolic resection in models of disease.

비디오: Murine Ileocolic Bowel Resection with Primary Anastomosis

Small Bowel Transplantation In Mice

Since 1990, the development of tacrolimus-based immunosuppression and improved surgical techniques, the increased array of potent immunosuppressive medications, infection prophylaxis, and suitable patient selection helped improve actuarial graft and patient survival rates for all types of intestine transplantation. Patients with irreversible intestinal failure and complications of parenteral nutrition should now be routinely considered for small intestine transplantation. However, Survival rates for small intestinal transplantation have been slow to improve compares increasingly favorably with renal, liver, heart and lung. The small bowel transplantation is still unsatisfactory compared with other organs. Further progress may depend on better understanding of immunology and physiology of the graft and can be greatly facilitated by animal models. A wider use of mouse small bowel transplantation model is needed in the study of immunology and physiology of the transplantation gut as well as efficient methods in diagnosing early rejection. However, this model is limited to use because the techniques involved is an extremely technically challenging. We have developed a modified technique. When making anastomosis of portal vein and inferior vena cava, two stay sutures are made at the proximal apex and distal apex of the recipient s inferior vena cava with the donor s portal vein. The left wall of the inferior vena cava and donor s portal vein is closed with continuing sutures in the inside of the inferior vena cava after, after one knot with the proximal apex stay suture the right wall of the inferior vena cava and the donor s portal vein are closed with continuing sutures outside the inferior vena cave with 10-0 sutures. This method is easier to perform because anastomosis is made just on the one side of the inferior vena cava and 10-0 sutures is the right size to avoid bleeding and thrombosis.  In this article, we provide details of the technique to supplement the video.

The mouse small bowel transplantation model has been recognized as an important tool to study mechanismes of immune rejection and screen new immunosuppressive drugs. However, this model is limited to use because the techniques involved is an extremely technically challenge. Now we introduce the modified technique.

마우스에 작은 창자 이식

Since 1990, the development of tacrolimus-based immunosuppression and improved surgical techniques, the increased array of potent immunosuppressive ...

연구

생물학

Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Intestinal resection is a common therapeutic approach for human diseases such as obesity, inflammatory bowel disease, Crohn's disease, and colon cancer that often results in severe short bowel syndrome-like adverse effects including bile acid diarrhea, dehydration, electrolyte disturbances, and nutrient malabsorption. Here we introduce a murine ileal resection model, termed ileectomy, to evaluate tissue communication and the maintenance of systemic homeostasis. After ileal resection, circulating blood is permanently devoid of the ileum-specific endocrine hormone fibroblast growth factor 15 (FGF15), which releases its endocrinal inhibition of bile acid synthesis in the liver. In combination with the increased production and abolished reabsorption of bile acids after removing the ileum, mice that underwent surgery suffer from bile salt overaccumulation in the intestine and associated diarrhea, morbidity, and mortality. Novel usage of the surgery model introduced in this study may provide mechanistic and functional insights into ileal control of systemic metabolic regulation in physiology and disease.

Small intestine-dependent bile acid reabsorption and feedback inhibition of hepatic bile acid synthesis is important for systemic homeostasis and health. In this study, we describe a mouse model for ileal resection to evaluate ileectomy-induced bile malabsorption, overaccumulation, and toxicity in mouse intestine.

Ileectomy 유도 담 즙 Overaccumulation 마우스 소장에서

Intestinal resection is a common therapeutic approach for human diseases such as obesity, inflammatory bowel disease, Crohn's disease, and colon cancer ...

의학

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Diversion colitis (DC) is a frequent clinical condition occurring in patients with bowel segments excluded from the fecal stream as a result of a diverting enterostomy. The etiology of this disease remains ill-defined but appears to differ from that of classical inflammatory bowel diseases such as Crohn's disease and ulcerative colitis. Research aimed to decipher the pathophysiological mechanisms leading to the development of this disease has been severely hampered by the lack of an appropriate murine model. This protocol generates a murine model of DC that facilitates the study of the immune system's role and its interaction with the microbiome in the development of DC. In this model using C57BL/6 animals, distal parts of the colon are excluded from the fecal stream by creating a distal colostomy, triggering the development of mild to moderate inflammation in the excluded bowel segments and reproducing the hallmark lesions of human DC with a moderate systemic inflammatory response. In contrast to the rat model, a large number of genetically-modified murine models on the C57BL/6 background are available. The combination of these animals with our model allows the potential roles of individual cytokines, chemokines, or receptors of bioactive molecules (e.g., interleukin (IL)-17; IL-10, chemokine CXCL13, chemokine receptors CXCR5 and CCR7, and the sphingosine-1-phosphate receptor 4) to be assessed in the pathogenesis of DC. The availability of congenic mouse strains on the C57BL/6 background largely facilitates transfer experiments to establish the roles of distinct cell types involved in the etiology of DC. Finally, the model offers the opportunity to assess the influences of local interventions (e.g., modification of the local microbiome or local anti-inflammatory therapy) on mucosal immunity in affected and non-affected bowel segments and the on systemic immune homeostasis.

Murine distal colostomy provides a murine model for human diversion colitis, a predominantly lymphocytic colitis in colon segment excluded from the fecal stream.

Murine 원심 인공, C57BL/6 마우스에 전환 Colitis의 새로운 모델

Diversion colitis (DC) is a frequent clinical condition occurring in patients with bowel segments excluded from the fecal stream as a result of a ...

Creation of Colonic Anastomosis in Mice

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of colonic surgeries and can occur in up to 26% of surgical anastomoses, with mortality being up to 39% for patients with such a leak. Currently, there remains a paucity of data detailing the cellular mechanisms of anastomotic healing. Devising preventative strategies and treatment modalities for anastomotic leak could be greatly potentiated by a better understanding of appropriate anastomotic healing.
A murine model is ideal as previous studies have shown that the murine anastomosis is the most clinically similar to the human case as compared with other animal models. We offer an easily reproducible murine model of colonic anastomosis in mice that will allow for further illustration of anastomotic healing.

Anastomotic leak or breakdown after surgery is a major cause of postoperative morbidity and mortality. Our surgery for creating a colonic anastomosis is a reliable and reproducible method for studying the healing mechanisms of anastomoses.

쥐에 있는 저 승 문 합의 창조

Intestinal anastomoses are commonly performed in both elective and emergent operations. Even so, anastomotic leaks are a highly feared complications of ...

Robotic Radical Cystectomy, Pelvic Lymph Node Dissection, and Intracorporeal Ileal Conduit Urinary Diversion

The robotic approach to radical cystectomy is compelling because of its oncologic equivalence to open radical cystectomy (ORC), its association with lower surgical blood loss, its potential association with shorter hospital stay after surgery. These factors suggest that the robotic approach to radical cystectomy may be an important component of enhanced recovery programs aimed at reducing surgical morbidity. This paper describes the importance of the cranial placement of robotic trocars, the use of Cadiere forceps for atraumatic bowel grasping, pelvic lymph node dissection (PLND), and utero-enteric anastomoses. Also discussed are steps that are critical for the successful outcome of RARC. In spite of the increased operating times and associated costs and the costs of robotic surgical platforms and equipment, adoption of the robotic technique by bladder cancer surgeons has increased. This paper describes a systematic and reproducible method that details robotic extended pelvic lymph node dissection, cystectomy/cystoprostatectomy, and intracorporeal ileal conduit urinary diversion.

This paper describes robotic radical cystectomy, pelvic lymph node dissection, and intracorporeal ileal conduit urinary diversion.

The robotic approach to radical cystectomy is compelling because of its oncologic equivalence to open radical cystectomy (ORC), its association with lower ...

<meta charset="utf8"></head><body>장 조작을 통한 수술 후 장폐색(Postoperative Ileus)을 연구하기 위한 쥐 모델(Murine Model for Study Postative Ileus through Intestinal Manipulation)

Postoperative Ileus Murine Model

Most patients experience postoperative ileus (POI) after surgery, which is associated with increased morbidity, mortality, and hospitalization time. POI is a consequence of mechanical damage during surgery, resulting in disruption of motility in the gastrointestinal tract. The mechanisms of POI are related to aberrant neuronal sensitivity, impaired epithelial barrier function, and increased local inflammation. However, the details remain enigmatic. Therefore, experimental murine models are crucial for elucidating the pathophysiology and mechanism of POI injury and for the development of novel therapies.
Here, we introduce a murine model of POI generated via intestinal manipulation (IM) that is similar to clinical surgery; this is achieved by mechanical damage to the small intestine by massaging the abdomen 1-3 times with a cotton swab. IM delayed gastrointestinal transit 24 h after surgery, as assessed by FITC-dextran gavage and fluorescence detection of the segmental digestive tract. Moreover, tissue swelling of the submucosa and immune cell infiltration were investigated by hematoxylin and eosin staining and flow cytometry. Proper pressure of the IM and a hyperemic effect on the intestine are critical for the procedure. This murine model of POI can be utilized to study the mechanisms of intestinal damage and recovery after abdominal surgery.

<meta charset="utf8"></head><body>수술 후 장폐색 쥐 모델

We describe a murine model of postoperative ileus generated via intestinal manipulation. Gastrointestinal transit function, pathologic changes, and immune cell activation were assessed 24 h after surgery.

수술 후 장폐색 쥐 모델

Most patients experience postoperative ileus (POI) after surgery, which is associated with increased morbidity, mortality, and hospitalization time. POI ...

면역학 및 감염병학

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Since their approval for clinical use, da Vinci surgical robots have shown great advantages in gastrointestinal surgical operations, especially in complex procedures. The high-quality 3-D visual, multijoint arm and natural tremor filtration allow the surgeon to expose and dissect more accurately with minimal invasion. Total mesorectal excision is the standard surgical technique for the treatment of resectable rectal cancer. To reduce the lateral recurrence rate, lateral pelvic lymph node dissection can be performed, as it is a safe and feasible procedure for locally advanced middle-low rectal cancer with a high possibility of metastasis to the lateral lymph nodes. However, the complexity of the anatomic structures and the high postoperative complication rate limit its application. Recently, several surgeons have increasingly used robotic techniques for total mesorectal excision and lateral pelvic lymph node dissection. Compared with open and laparoscopic surgery, the robotic technique has several advantages, such as less blood loss, fewer blood transfusions, minimal trauma, shorter postoperative hospitalization, and quicker recovery. A robotic approach is generally regarded as a reasonable alternative for complicated procedures such as lateral pelvic lymph node dissection, although there are a limited number of high-quality prospective randomized controlled studies reporting direct evidence. Here, we provide the detailed steps of robot-assisted total mesorectal excision and lateral pelvic lymph node dissection performed at the First Affiliated Hospital of Xi&#39;an Jiaotong University.

The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.

로봇 보조 총 중각 절제술 및 측면 골반 림프절 해부 국소 고급 중간 낮은 직장 암

Since their approval for clinical use, da Vinci surgical robots have shown great advantages in gastrointestinal surgical operations, especially in complex ...

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes mellitus, dyslipidemia, non-alcoholic steatohepatitis, cardiovascular events, and cancers) through restrictive and malabsorptive mechanisms. Understanding the mechanisms by which these procedures allow such improvements often require their transposition into animals, especially in mice, because of the ease of generating genetically modified animals. Recently, the single-anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S) has emerged as a procedure that uses both restrictive and malabsorptive effects, which is being used as an alternative to gastric bypass in case of major obesity. Thus far, this procedure has been associated with strong metabolic improvements, which has led to a marked increase in its use in daily clinical practice. However, the mechanisms underlying these metabolic effects have been poorly studied as a result of a lack of animal models. In this article, we present a reliable and reproducible model of SADI-S in mice, with a special focus on perioperative management. The description and use of this new rodent model will be helpful for the scientific community to better understand the molecular, metabolic, and structural changes induced by the SADI-S and to better define the surgical indications for clinical practice.

Single-anastomosis duodeno-ileal bypass (SADI-S) is an emerging bariatric procedure with important metabolic effects. In this article, we present a reliable and reproducible model of SADI-S in mice.

생쥐의 단일 문합 십이지장-회장 우회술(Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice)

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes ...

차 문합와 쥐의 Ileocolic 대장 절제술

요약

더 많은 비디오 탐색

이 비디오의 챕터

마우스에 작은 창자 이식

Ileectomy 유도 담 즙 Overaccumulation 마우스 소장에서

Murine 원심 인공, C57BL/6 마우스에 전환 Colitis의 새로운 모델

쥐에 있는 저 승 문 합의 창조

Robotic Radical Cystectomy, Pelvic Lymph Node Dissection, and Intracorporeal Ileal Conduit Urinary Diversion

수술 후 장폐색 쥐 모델

수술 후 장폐색 쥐 모델

수술 후 장폐색 쥐 모델

로봇 보조 총 중각 절제술 및 측면 골반 림프절 해부 국소 고급 중간 낮은 직장 암

생쥐의 단일 문합 십이지장-회장 우회술(Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice)