Name: high throughput assay to examine egg-laying preferences of individual drosophila melanogaster
Uploaded: 2016-03-24T13:00:00.000+00:00
Duration: 3 min 58 s
Description: Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

We thank the Duke Physics Shop, especially Phil Lewis, for helping us build the behavioral apparatus and attachments and creating the drawing. This work is funded by the National Institutes of Health under award number R01GM100027.

1. إعداد الذباب إلى أن يعاير <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ثقافة الذباب على العسل الأسود القياسية / وسائل الإعلام دقيق الذرة في مجموعة حاضنة عند 25 درجة مئوية والرطوبة 65٪. الحرص على عدم ازدحام قارورة. على سبيل المثال، وضعت 8 إناث و 6 ذكور في قارورة الغذاء الضيقة. ملاحظة: &quot;قارورة الغذاء الضيقة&quot; المستخدمة هنا ديها قطرها الداخلي 2.3 سم. ونحن عادة الاستغناء عن 10 مل من المواد الغذائية يطير في كل قارورة. ووصف وصفة الطعام ذبابة كنا هنا: http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.html. </li><li style=";text-align:right;direction:rtl"> 2 - بعد 3 أيام من الإناث eclosion، وإعداد قارورة مع عجينة الخميرة الطازجة كما هو مبين في الشكل 1E. جعل الطازجة عجينة الخميرة عن طريق خلط 6 غرام من الخميرة النشطة مع 10 مل من حمض proprionic 0.5٪. استخدام ملعقة لتطبيق عجينة الخميرة على جدار من قارورة الغذاء. جمع 30-35 الإناث eclosed جنبا إلى جنب مع 20 - 25 من الذكور في قارورة. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> بشكل عام، وجمع الذكور من نفس قارورة حيث يتم جمع الإناث(وذلك لتوفير الوقت والجهد). ومع ذلك، إذا كان هناك قلق حول خصوبة الذكور، استخدام الذكور WT بدلا من ذلك. أيضا، الخميرة لصق مهم لتحفيز إنتاج البيض ومن ثم إعدادها طازجة يوميا. </li></ol></li><li style=";text-align:right;direction:rtl"> الحفاظ على جمع الإناث / الذكور عند 25 درجة مئوية، الرطوبة 65٪، إلا الذباب هي درجة الحرارة حساسة. </li><li style=";text-align:right;direction:rtl"> بعد ~ 4-5 أيام، والتحقق من قوارير لمعرفة ما اذا الإناث جاهزة للتجارب وضع البيض. انهم مستعدون عند سطح المتوسطة الغذاء الرطب من اليرقات تختبئ بنشاط في الغذاء (انظر الشكل 1E - F). ملاحظة: الإناث عادة الامتناع عن وضع البيض مرة واحدة يصبح سطح الغذاء الرطب والزحف مع اليرقات. تضمن هذه الخطوة أن الإناث ستكون جاهزة لوضع الكثير من البيض عندما كانت تواجه ركائز مع الملمس المطلوب (على سبيل المثال، ~ 1 الاغاروز٪). </li></ol> 2. غرفة البناء، الجمعية، إعداد الفحص <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> لدينا متجر آلة بناء غرف وضع البيض الاكريليك (فايجوري 1A - D). وظهر الرسم الهندسي من قطع مختلفة في الشكل التكميلي 1 - 3 يمكن العثور عليها أيضا صور عالية الدقة هنا (http://www.rebeccayang.org/pdf/chamber٪20design.pdf). </li><li style=";text-align:right;direction:rtl"> إدراج الأغطية البلاستيكية إلى تحميل (أعلى) قطعة من الغرفة كما هو مبين في الشكل 1C. وهذا بمثابة السطح السفلي بينما الذباب تحميل في الساحات وضع البيض على حدة. </li><li style=";text-align:right;direction:rtl"> تخدير الإناث على وسادة CO 2 وتحميلها بشكل فردي في كل الساحة وضع البيض. سماح ~ 30 دقيقة للذباب للتعافي من CO 2 وتصبح تأقلم مع البيئة الجديدة. </li><li style=";text-align:right;direction:rtl"> تحضير ركائز الاغاروز. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> للراحة، والحفاظ على زجاجة ولم يضف من ذاب 1٪ الاغاروز في 55 ° C حمام الماء. </li><li style=";text-align:right;direction:rtl"> إضافة المبلغ المطلوب من محلول المخزون السكروز (2 م) إلى 50 مل أنبوب مخروطي الشكل وخلطها مع كمية مناسبة من الاغاروز. على سبيل المثال، لإعداد 150 ملي السكروز الركيزة، جيش التحرير الشعبى الصينىم 750 ميكرولتر من 2 M حل السكروز في أنبوب ثم ملء الأنبوب مع الاغاروز إلى علامة 10 مل. </li><li style=";text-align:right;direction:rtl"> إعداد الركيزة سهل بنفس الطريقة ولكن مع إضافة الماء المقطر بدلا من محلول السكروز. ملاحظة: تركيز النهائي من الاغاروز في هذا البروتوكول هو قليلا أقل من 1٪. في تجربتنا، والتركيز الدقيق الاغاروز لا يهم طالما تسيطر عليه أن يكون في حدود ~ 0،9-1،1٪ وأن ركائز هما من تركيز الاغاروز نفسه. </li></ol></li><li style=";text-align:right;direction:rtl"> خذ قطعة الركيزة (القاع) من الغرفة وماصة 1000 ميكرولتر من الركيزة الأغاروس في كل الحوض كما رأينا في الشكل 1D. </li><li style=";text-align:right;direction:rtl"> السماح للالاغاروز لترسيخ ب ~ 30 دقيقة. </li><li style=";text-align:right;direction:rtl"> مرة واحدة ركائز الاغاروز والذباب جاهزة، تجميع كل الأجزاء الثلاثة من الغرفة وضع البيض ثم تأخذ بها الأغطية البلاستيكية. </li><li style=";text-align:right;direction:rtl"> ضع الدوائر في حاضنات الطاير. ملاحظة: طول التجارب وضع البيض يمكن أن تختلف تبعا بةاحتياجات rimental. ونحن عادة تشغيل التجربة O / N (14-16 ساعة). أيضا، لم يلاحظ أي تأثير كبير على توقيت الساعة البيولوجية على تفضيلات وضع البيض. </li><li style=";text-align:right;direction:rtl"> تخدير عن طريق حقن الإناث CO 2 في الغرفة. تفكيك الغرفة، تجاهل الذباب تخدير في مشرحة ذبابة (أي، يمكن للقهوة فارغة مليئة بعض زيت الذرة). التقاط صور من نتائج السجلات (انظر الشكل 2). </li><li style=";text-align:right;direction:rtl"> حساب عدد البيض يدويا وحساب مؤشرات تفضيل للتحليل. حساب مؤشر التفضيل (N A - N ب) / (N + N لب) حيث ن أ و ن ب يمثلون عدد من البيض في الموقع موقع مقابل ب، على التوالي. </li></ol>

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The authors have nothing to disclose.

الدوائر والبروتوكولات المذكورة هنا لديهم العديد من التحسينات على المقايسات وضع البيض السابقة. أولا، أنها تزيد من الإنتاجية من يعاير تفضيلات من الحيوانات وحيدة بشكل ملحوظ. كل غرفة يمكن فحص 30 من الإناث واحدة ويستغرق أقل من ساعة لاقامة. ثانيا، أنها تزيد من الاتساق في ت?...

Drosophila melanogaster is a powerful genetic model organism to study the neural basis of behaviors. The rapid developments of genetic tools to manipulate neurons in a targeted manner and the emergence of sophisticated behavioral analysis tools have significantly improved our ability to dissect the circuit mechanisms that underlie the sensory-motor transformation processes of several innate and learned behaviors1-3.
Drosophila egg-laying is a suitable model to study the neural basis of simple decision making processes. In particular, Drosophila females have been shown to possess the ability to compare and rank their options before "committing" to depositing an egg onto a given option4-8. For example, when given only a plain (sucrose-free) substrate or only a sucrose-containing substrate, females readily accept either option for egg-laying. However, when presented with both options, females robust reject the sucrose substrate in some contexts7,9,10. Relatively little is known about the neural mechanisms that allow females to "choose the greater of two goods", however. A major obstacle has been the lack of an efficient method to assay egg-laying preferences such that one can use a systematic genetic screening approach to study this problem.
In this report, we describe the protocol we developed that allows egg-laying preferences of females to be assayed at single-animal resolution and with substantially improved throughput and consistency over previous methods. Specifically, we provide the blueprints for constructing the chambers we designed, the protocol for preparing the females so that each is primed to lay many eggs, and the protocol for using the chambers.

<table><tbody><tr><td>UltraPure Agarose&amp;nbsp;</td><td>Invitrogen</td><td>16500-500</td><td></td></tr><tr><td>Sucrose</td><td>Sigma</td><td>S0389</td><td></td></tr><tr><td>Water bath&amp;nbsp;</td><td>Fisher</td><td>15-462-6Q</td><td></td></tr><tr><td>LifeCam Cinema webcam</td><td>Microsoft</td><td>H5D-00013</td><td></td></tr><tr><td>Red LEDs</td><td>Cree</td><td>C503B-RAN-CA0B0AA1</td><td></td></tr><tr><td>Egg-laying chambers</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Camera holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>LED holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Fly vials (narrow)</td><td>Genesee</td><td>32-116BC</td><td></td></tr></tbody></table>

high throughput assay to examine egg-laying preferences of individual drosophila melanogaster

في الآونة الأخيرة، ظهرت أفضلية وضع البيض ذبابة الفاكهة نموذجا لين العريكة وراثيا لدراسة الأساس العصبي للعمليات صنع القرار بسيطة. عند اختيار مواقع لإيداع بيضها، الذباب الإناث قادرة على المرتبة الجاذبية النسبية للخيارات واختيار &quot;أكبر من سلعتين.&quot; ومع ذلك، فإن معظم فحوصات تفضيل وضع البيض ليست عملية إذا كان أحد يريد أن يأخذ نهجا الفحص الجيني المنهجي للبحث عن أساس الدوائر الكامنة وراء هذه العملية صنع القرار بسيطة، كما هي وشاقة لانشاء القائمة على السكان. لزيادة الإنتاجية دراسة الأفضليات وضع البيض من الإناث واحدة، وضعنا غرف مخصصة كل علبة تفضيلات وضع البيض الفحص في وقت واحد لمدة تصل إلى ثلاثين فرد الذباب وكذلك البروتوكول الذي يضمن لكل امرأة لديها نسبة وضع البيض عالية (بحيث تفضيلها هو ملحوظ بسهولة وأكثر إقناعا). نهجنا هو بسيط لتنفيذوتعطي نتائج متسقة للغاية. بالإضافة إلى ذلك، يمكن تجهيز هذه الغرف مع المرفقات مختلفة للسماح تسجيل فيديو للحيوانات وضع البيض ولتسليم ضوء دراسات علم البصريات الوراثي. وتنص هذه المادة على مخططات لافتعال هذه الدوائر وإجراءات إعداد الذباب إلى أن يعاير في هذه الدوائر.

وتتكون غرف وضع البيض من عدة قطع: ركيزة (القاع) قطعة، قطعة المفرق (وسط)، وتحميل (أعلى) قطعة، و 2 أبواب منزلقة (الشكل 1A - D). وتستخدم هذه القطع بشكل مستقل الذباب الإعداد وركائز قبل التجارب وضع البيض. ويبين الشكل 1F كيف قارورة ينبغي أن ننظر ع...

Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

عالية الإنتاجية الفحص لفحص تفضيلات بيضة زرع الفردي<em&gt; ذبابة الفاكهة</em

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

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Neuroscience

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

7.9K Views.  Duke University. This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Video: High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Methods to Assay Drosophila Behavior

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases1. We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials2-4. The rapid iterative negative geotaxis (RING) assay5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments.

Methods to Assay Drosophila Behavior

Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.

طرق الفحص ذبابة الفاكهة سلوك

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer basic questions on how light information is processed and shared between rapid and circadian behaviors. Drosophila larvae display a stereotypical avoidance behavior when exposed to light. To investigate light dependent behaviors comparably simple light-dark preference tests can be applied. In vertebrates and arthropods the neural pathways involved in sensing and processing visual inputs partially overlap with those processing photic circadian information. The fascinating question of how the light sensing system and the circadian system interact to keep behavioral outputs coordinated remains largely unexplored. Drosophila is an impacting biological model to approach these questions, due to a small number of neurons in the brain and the availability of genetic tools for neuronal manipulation. The presented light-dark preference assay allows the investigation of a range of visual behaviors including circadian control of phototaxis.

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Here we describe a light-dark preference test for Drosophila larva. This assay provides information about innate and circadian regulation of light sensing and processing photobehavior.

ضوء الفحص ميول لدراسة الساعة البيولوجية الفطرية وPhotobehavior الخاضعة للرقابة في<em&gt; ذبابة الفاكهة</em&gt; يرقات

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

واحدة ذبابة الفحص لسلوك البحث عن الطعام في<em&gt; ذبابة الفاكهة</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous system and protects neural tissue from toxins and pathogens. Defects in the formation of this barrier have been correlated with neuropathies, and the breakdown of this barrier has been observed in many neurodegenerative diseases. Therefore, it is critical to identify the genes that regulate the formation and maintenance of the blood-brain barrier to identify potential therapeutic targets. In order to understand the exact roles these genes play in neural development, it is necessary to assay the effects of altered gene expression on the integrity of the blood-brain barrier. Many of the molecules that function in the establishment of the blood-brain barrier have been found to be conserved across eukaryotic species, including the fruit fly, Drosophila melanogaster. Fruit flies have proven to be an excellent model system for examining the molecular mechanisms regulating nervous system development and function. This protocol describes a step-by-step procedure to assay for blood-brain barrier integrity during the embryonic and larval stages of D. melanogaster development.

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

فحص لسلامة حاجز الدم في الدماغ في دروسوفيلا melanogaster

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous ...

Developmental Biology

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense organs provide an excellent model system for the study of mechanosensation. This protocol describes a simple touch behavior in fruit flies which can be used to identify mutations that interfere with mechanosensation. The tactile stimulation of a macrochaete bristle on the thorax of flies elicits a grooming reflex from either the first or third leg. Mutations that interfere with mechanotransduction (such as NOMPC), or with other aspects of the reflex arc, can inhibit the grooming response. A traditional screen of adult behaviors would have missed mutants that have essential roles during development. Instead, this protocol combines the touch screen with mosaic analysis with a repressible cell marker (MARCM) to allow for only limited regions of homozygous mutant cells to be generated and marked by the expression of green fluorescent protein (GFP). By testing MARCM clones for abnormal behavioral responses, it is possible to screen a collection of lethal p-element mutations to search for new genes involved in mechanosensation that would have been missed by more traditional methods.

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

النسخ في والسلوكية الفحص لMechanosensation من MARCM المستندة<em&gt; ذبابة الفاكهة السوداء البطن</em

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense ...

Measuring and Altering Mating Drive in Male Drosophila melanogaster

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, reductionist, and scalable system for in-depth investigation of motivation using the mating drive of male Drosophila&#160;melanogaster (Drosophila), the methods for which we detail here. The behavioral paradigm centers on the finding that male mating drive decreases alongside fertility over the course of repeated copulations and recovers over ~3 d. In this system, the powerful neurogenetic tools available in the fly converge with the genetic accessibility and putative wiring diagram available for sexual behavior. This convergence allows rapid isolation and interrogation of small neuronal populations with specific motivational functions. Here we detail the design and execution of the satiety assay that is used to measure and alter courtship motivation in the male fly. Using this assay, we also demonstrate that low male mating drive can be overcome by stimulating dopaminergic neurons. The satiety assay is simple, affordable, and robust to influences of genetic background. We expect the satiety assay to generate many new insights into the neurobiology of motivational states.

Measuring and Altering Mating Drive in Male Drosophila melanogaster

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

قياس وتغيير التزاوج محرك في ذكر<em&gt; ذبابة الفاكهة</em

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, ...

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which enables them to seek out their preferred thermal landscape. To define the temperature preferences of larvae over a defined linear range, we developed an assay using a temperature gradient. To establish a single-directional gradient, two aluminum blocks are connected to independent water baths, each of which controls the temperature of individual blocks. The two blocks set the lower and upper limits of the gradient. The temperature gradient is established by placing an agarose-coated aluminum plate over the two water-controlled blocks so that the plate spans the distance between them. The ends of the aluminum plate that is set on the top of the water blocks defines the minimum and maximum temperatures, and the regions in-between the two blocks form a linear temperature gradient. The gradient assay can be applied to&#160;larvae of different ages and can be used to identify mutants that exhibit phenotypes, such as those with mutations affecting genes encoding TRP channels and opsins, which are required for temperature discrimination.

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

مقايسة تدرج درجة الحرارة لتحديد تفضيلات الحرارية يرقات المورفولوجية

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

A سريع الغذاء تفضيل المقايسة في دروسوفيليا

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

Source: Lee, S. Y. and Kang, K. <a href="https://jove.unicartagenaproxy.elogim.com/video/60419/measuring-effects-bacteria-chemicals-on-intestinal-permeability">Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans</a>.&#160;J. Vis. Exp. (2017).
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies.&#160; This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive toxicity.

فحص وضع البيض: طريقة لتحديد سلوك وضع البيض ل C. ايليجانس

Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. 
...

JoVE Encyclopedia of Experiments

Biology

Caenorhabditis elegans (worm)

Basic Methods

Island Assay: A High-Throughput Method to Evaluate Drosophila Locomotor Behavior

Source: Eidhof, I., et al. <a href="https://jove.unicartagenaproxy.elogim.com/video/55892/" target="_blank">High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay</a>. J. Vis. Exp. (2017).
This video describes the island assay, a behavioral method used to study Drosophila locomotion. The sample protocol features a setup for the assay compatible with semi-automated image analysis.

فحص الجزيرة: طريقة عالية الإنتاجية لتقييم السلوك الحركي ذبابة الفاكهة

Source: Eidhof, I., et al. High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay. J. Vis. Exp. (2017).
This video describes the ...

عالية الإنتاجية الفحص لفحص تفضيلات بيضة زرع الفردي

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Chapters in this video

طرق الفحص ذبابة الفاكهة سلوك

ضوء الفحص ميول لدراسة الساعة البيولوجية الفطرية وPhotobehavior الخاضعة للرقابة في

واحدة ذبابة الفحص لسلوك البحث عن الطعام في

فحص لسلامة حاجز الدم في الدماغ في دروسوفيلا melanogaster

النسخ في والسلوكية الفحص لMechanosensation من MARCM المستندة

قياس وتغيير التزاوج محرك في ذكر

مقايسة تدرج درجة الحرارة لتحديد تفضيلات الحرارية يرقات المورفولوجية

A سريع الغذاء تفضيل المقايسة في دروسوفيليا

فحص وضع البيض: طريقة لتحديد سلوك وضع البيض ل C. ايليجانس

فحص الجزيرة: طريقة عالية الإنتاجية لتقييم السلوك الحركي ذبابة الفاكهة