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Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

3.0K Views

08:15 min

February 3rd, 2022

DOI :

10.3791/63346-v

February 3rd, 2022


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Mitochondrial Energy Metabolism

Chapters in this video

0:05

Introduction

1:03

Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology

2:44

Preparation of XF Assay Medium

3:08

Preparation of the Sensor Cartridge

4:25

Seeding Single Spheroids into the Spheroid Assay Microplate

5:04

Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays

5:31

Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids

7:35

Conclusion

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