Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
2.5K Views
•
10:17 min
•
September 24th, 2021
DOI :
September 24th, 2021
•1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor
Chapters in this video
0:05
Introduction
1:08
Hydration of the Sensor Cartridge
2:03
Preparation of Cell-Adhesive-Coated Microplates
2:52
Seeding Cells in Cell-Adhesive-Coated Microplates
4:10
Glycolysis Stress Test Using the Extracellular Flux Analyzer
7:53
Results: Assessment of the Glycolytic Function and Mitochondrial Respiration in Mouse HSPCs
9:36
Conclusion
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