Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy
1.9K Views
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11:12 min
•
December 1st, 2021
DOI :
December 1st, 2021
•Nikita V. Zhilyakov1, Arsenii Y. Arkhipov1, Eduard F. Khaziev1,2, Marat A. Mukhamedyarov3, Dmitry V. Samigullin1,2
1Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology, Kazan State Medical University
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Chapters in this video
0:05
Introduction
1:12
Dye Loading Procedure
3:28
Video Capture with Confocal Microscopy
6:14
Video Processing
7:56
Video Analysis
9:55
Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
10:18
Conclusion
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