Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

1:17

Dissection of Muscles and Immunostaining

3:21

Image Acquisition

3:58

Image Analysis to Calculate Postsynaptic NMJ Endplate Volume, MIP Area, and Relative Tortuosity

5:05

Image Analysis to Quantify Presynaptic Neurofilament Accumulation and Synaptic Vesicle Glycoprotein 2 Staining

6:04

Calculating the Distance Between Acetylcholine Receptor (AChR) Stripes and Their Width

8:22

Results: Multi Parameter Postsynaptic NMJ Characterization, STED Imaging, and Morphometric Analysis of Presynaptic Axon Terminal Distribution

10:09

Conclusion