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GM-Free Generation of Blood-Derived Neuronal Cells

2.9K Views

08:11 min

February 13th, 2021

DOI :

10.3791/61634-v

February 13th, 2021


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GM Free Generation

Chapters in this video

0:04

Introduction

0:47

Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs

1:25

Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs

2:21

Sorting of Newly Generated Dedifferentiated Cells

3:33

Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells

4:10

Culturing of Neuronal Differentiated Blood Cells

4:44

Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells

6:08

Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells

7:41

Conclusion

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