This work was supported by internal funds of the Department of Neurosurgery, Brown University to N.T.

The protocols for collection, isolation, and propagation of patient-derived human glioma cells were approved by the IRB committee of Rhode Island Hospital. All animals were maintained according to the NIH Guide for the Care and Use of Laboratory Animals. All animal use protocols were approved by the Institutional Animal Care and Use Committee of Rhode Island Hospital.
1. Media and buffer preparations
<ol>
	<li>Prepare 50 mL of Neurosphere Media: 1x Neuronal basal medium w/o vitamin A, 1x serum free supplement w/o vitamin A, 2 mM L-glutamine, 20 ng/mL epidermal growth factor (EGF), 20 ng/mL basic-fibroblast growth factor (FGF), 2 &#181;g/mL heparin, and 1x antibiotic-antimycotic (anti-anti).</li>
	<li>Prepare 50 mL of supplemented Neuronal Basal Medium: 1x Neuronal basal medium, 1x serum free supplement, 4 g/L D-glucose, 2 mM L-glutamine, and 50 ng/mL nerve growth factor (NGF).</li>
	<li>Prepare 500 mL of N2B2 Medium: 1:1 Dulbecco&#39;s Modified Eagle Medium-Ham&#39;s F12 Nutrient Mixture (DMEM-F12), 1x Insulin-Transferrin-Selenium (ITS-G), 66 mg/mL bovine serum albumin (BSA), 0.1 mg/mL transferrin, 0.01 mg/mL biotin, 6.29 mg/mL progesterone, 5 &#181;g/mL N-Acetyl-L-cystine (NAC), and 1 mM putriscine. Aliquot and freeze at -20 &#176;C.</li>
	<li>Prepare 500 mL of C-Medium: 1x Minimum Essential Medium (MEM), D-glucose (final 4 g/L), 10% fetal bovine serum (FBS), and 2 mM L-glutamine. Aliquot and freeze at -20 &#176;C. Add nerve growth factor (NGF) fresh before use (50 ng/mL).</li>
	<li>Prepare 200 mL of Papain Buffer: 1x Earle&#39;s Balanced Salt Solution (EBSS), 100 mM Mg2SO4, 30% Glucose, 0.25 M EGTA, and 1 M NaHCO3.</li>
	<li>Prepare 10 mL of DMEM-ITS-G Medium: 1x DMEM, 0.5% BSA, and 1x ITS-G.</li>
	<li>Prepare 200 mL of PB Buffer: 1x Dulbecco&#39;s phosphate-buffered saline (DPBS) without Ca2+ and Mg2+ and 0.5% BSA. Degas the buffer before use and keep on ice.</li>
</ol>
2. Isolation and culture of Glioma Stem Cell Neurospheres
<ol>
	<li>Isolation of Neurospheres
	<ol>
		<li>Collect IRB approved and patient consented fresh human glioblastoma (GBM) tissue from the operating room. Transfer GBM samples on ice in DPBS containing 2x anti-anti to a BL2 certified biological safety cabinet.</li>
		<li>Transfer one 1 cm3 GBM sample with minimal necrosis or red blood cell contamination to a 60 mm plate and cut into 1 mm3 fragments; remove any excess DPBS.</li>
		<li>Digest the tissue with 5 mL of collagenase/dispase (1 mg/mL) for 30 min at 37 &#176;C and gently swirl the dish every 5-10 min.</li>
		<li>Transfer digested fragments to a 50 mL tube and triturate by pipetting with a 10 mL pipette several times to dissociate the tissue.</li>
		<li>Add an equal volume of Neurosphere Media and triturate the tissue again; allowing the large fragments to settle.</li>
		<li>Remove media without tissue fragments and pass slowly through a 70 &#181;M cell strainer placed over a fresh 50 mL tube.</li>
		<li>Repeat steps 2.1.5-2.1.6 until all the tissue has been dissociated, changing the cell strainer as needed.</li>
		<li>Spin the cell suspension at 110 x g for 10 min.</li>
		<li>Remove the supernatant and resuspend the pellet in 10 mL of ACK lysing buffer to remove red blood cell contamination. Incubate for 10 min at room temperature.</li>
		<li>Spin the cell suspension at 110 x g for 5 min.</li>
		<li>Remove the supernatant and resuspend cells in 10 mL of Neurosphere Media. Take 10 &#181;L of the cell suspension and dilute with 10 &#181;L of trypan blue. Count 10 &#181;L of the cell suspension using an automated cell counter or hemocytometer and plate in 10 mL of Neurosphere Media at a density of 3 x 106 cells in 100 mm suspension culture plates.</li>
		<li>Add 2 mL of fresh Neurosphere Media to the culture 2-3 times a week. 
		NOTE: Neurospheres will form in suspension within 3-4 weeks. After subculturing for 2-4 times, confirm the stemness via the limiting dilution assay and tumor recapitulation via xenographic transplantation in immunocompromised mice as previously described 22.</li>
	</ol>
	</li>
	<li>Sub-culturing Neurospheres
	<ol>
		<li>When spheres form and reach a diameter between 200-500 &#181;m, transfer neurospheres to a 15 mL tube and spin at 110 x g for 5 min.</li>
		<li>Resuspend neurospheres in 1 mL of pre-warmed cell detachment solution.</li>
		<li>Transfer to a 1.5 mL tube and incubate at 37 &#176;C for 5 min.</li>
		<li>Set a P200 pipette to 150 &#181;L and triturate by pipetting up and down to dissociate neurospheres.</li>
		<li>Transfer dissociated cells to a 15 mL tube. Add 4 mL of Neurosphere Media and spin at 300 x g for 5 min.</li>
		<li>Remove the supernatant and resuspend cells in 5 mL of Neurosphere Media. Take 10 &#181;L of the cell suspension and dilute with 10 &#181;L of trypan blue. Count 10 &#181;L of the cell suspension using an automated cell counter or hemocytometer and plate at a density of 1 x 106 cells/60 mm dish in a final volume of 4 mL.</li>
		<li>Refresh media 2 times a week and subculture cells as needed. Freeze whole neurospheres when desired in Neuronal basal media w/o vitamin A supplemented with 10% DMSO. Neurospheres may be used for experiments after P4.</li>
	</ol>
	</li>
</ol>
3. Compartmented culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs) and hGCs
<ol>
	<li>Preparation of compartmented culture dishes 
	NOTE: Perform the following steps the days before the planned harvest of the DRGs.
	<ol>
		<li>Assemble compartmented culture dishes.</li>
		<li>Dilute collagen stock solution to 500 &#181;g/mL in sterile distilled H2O; mix thoroughly.</li>
		<li>With a sterile transfer pipette, fill a 35 mm culture dish with 2 mL of collagen solution; remove the solution, leaving a thin film of collagen behind and place it into the next 35 mm dish. Repeat this process, adding more collagen solution as needed, until all dishes have been coated.</li>
		<li>Once all plates are coated, place the plates in a 245 mm x 245 mm culture tray and lay three 1 mm x 1 mm gauze pads in the center of the tray.</li>
		<li>To polymerize the collagen, wet gauze pads with 1 mL of concentrated ammonium hydroxide and cover the trays for 15 min.</li>
		<li>Remove gauze pads and allow the 35 mm dishes to dry in the laminar flow hood.</li>
		<li>While dishes are drying, load the barrel of the syringe grease applicator with high vacuum grease. Place the compartmented chambers in a large mouth media bottle filled with distilled water. Sterilize both by autoclaving and allow to cool.</li>
		<li>File off the point of an 18-G needled to make a blunt tip. Sterilize in 70% ethanol. Attach the needle to the grease syringe.</li>
		<li>Sterilize the pin rake by soaking in 70% ethanol; allow it to air-dry in the laminar flow hood.</li>
		<li>Remove the lid from a dry, collagen coated 35 mm dish. Hold the dish between the thumb and pointer finger. Hold the pin rake with the other hand. Apply a firm pressure to create even 200 &#181;M wide scratches across the center of the dish.</li>
		<li>Using a pasture pipette, place two drops of Supplemented Neuronal basal Medium in the center of the scratches.</li>
		<li>Repeat steps 3.1.10-3.1.11 until all dishes have been scratched.</li>
		<li>Dry the compartmented chambers in a laminar flow hood.</li>
		<li>With sterile hemostatic forceps, grasp one compartmented chamber by the center divider. Flip the hemostatic forceps so the bottom of the chamber is facing up.</li>
		<li>Apply silicone grease to the compartmented chamber, starting at the top. Ensure that grease is placed neatly and overlaps at all corners.</li>
		<li>Remove the lid from a 35 mm dish. Invert the dish and place the scratches over the chamber. Tap down on the bottom of the plate gently with a pair of forceps.</li>
		<li>Gently flip the plate over by using the hemostatic forceps. Release the forceps.</li>
		<li>Place a mound of grease at the base of the center compartment. Fill each chamber with Supplemented Neuronal Basal Medium (NB) and check for leaks. Seal leaks with silicone grease as needed.</li>
		<li>Continue assembling all culture dishes and store overnight at 37&#176;, 5% CO2.</li>
	</ol>
	</li>
	<li>Isolation of Rat DRG Neurons and Culture in the Compartmented Chamber
	<ol>
		<li>Sacrifice a timed pregnant E16 Sprague-Dawley rat by CO2 asphyxiation or by chemical overdose.</li>
		<li>Place the animal in the supine position on a clean surface; disinfect the abdomen with 70% ethanol.</li>
		<li>Grasp the skin of the lower abdomen with forceps and lift gently.</li>
		<li>Using scissors, make an &#34;I&#34; incision along the midline of the animal, taking care not to puncture the muscles of the abdominal wall.</li>
		<li>With a clean pair of forceps, grasp the muscle wall and make a transverse incision with a clean pair of scissors using caution not to puncture the uterus or intestines.</li>
		<li>With a pair of blunt forceps, grasp the uterus and gently lift straight up out of the peritoneal cavity.</li>
		<li>Using fresh, sterile scissors, clip the connective tissue at the base of each uterine horn.</li>
		<li>Place the entire uterus in a sterile 100 mm tissue culture dish.</li>
		<li>Carry the uterus to a laminar flow hood for dissection.</li>
		<li>Remove embryos from the uterus, one at a time, by clipping through the amniotic sac and gently teasing the embryo out and into a 60 mm dish containing 5 mL of L-15 with 1x pen-strep.</li>
		<li>With fine forceps, place 3-4 embryos into a 60 mm dish containing 5 mL of L-15 with 1x pen-strep.</li>
		<li>Working under a dissecting microscope and with one embryo at a time, euthanize by decapitation.</li>
		<li>Lie the embryo ventral side up and remove the limbs and the tail.</li>
		<li>With fine forceps, make a midline incision in the animal.</li>
		<li>Remove internal organs and tissue to expose the dorsal structures, particularly the spinal cord which should be visible upon completion.</li>
		<li>Place one blade of micro-dissecting scissors between the vertebral column and the spinal canal and carefully cut through the vertebral column to expose the spinal cord. Take care not to clip through the spinal cord.</li>
		<li>With fine forceps, lightly grasp the rostral end of the spinal cord and slowly lift out of the embryo. The DRGs will be attached to the spinal cord.</li>
		<li>Transfer spinal cords and attached DRGs to a 35 mm dish containing 2 ml of L-15 with 1x pen-strep. Place on the ice.</li>
		<li>Once all spinal cords have been isolated, use fine forceps to individually pluck DRGs from the spinal cord. Place DRGs into a fresh 35 mm dish.</li>
		<li>If nerve roots are present on DRGs, clip them away.</li>
		<li>Remove prepared 35 mm dishes from the 37 &#176;C, 5% CO2 incubator. Remove the media from the previous day. Place 80 &#181;L of Supplemented Neuronal Basal Media (NBF) containing 10 &#181;M 5-Fluoro-2&#39;-deoxyuridine (FUDR) in the center compartment and 250 &#181;L of media in each outer compartment.</li>
		<li>Place 2 ganglia in each center compartment. Return dishes to the 37 &#176;C, 5% CO2 incubator.</li>
		<li>The following day add 2.5 mL of NBF.</li>
		<li>Feed the cultures following the schedule in Table 1, altering the schedule based on the day chosen to begin the DRG prep.</li>
		<li>On day 21 (or when axons reach the end of the distal compartments), either seed cultures with a GBM neurosphere (Proceed to step 3.2.26) and live image or myelinate the cultures with oligodendrocytes cultures (Proceed to step 3.3) and then seed with a GBM neurosphere after myelination.</li>
		<li>Replace Supplemented NB Medium in each distal compartmented chamber that will be seeded with a GBM neurosphere with Supplemented NB Medium containing 10% FBS.</li>
		<li>With a P20 pipette set to 10 &#181;L, remove one GBM neurosphere from the culture dish. The GBM neurosphere should measure approximately 200 microns in size.</li>
		<li>Place the tip of the pipette in the distal chamber and expel the GBM neurosphere slowly so that it gently falls onto the axons in the portion of the distal chamber that is closest to the center chamber, over the axons near the center chamber. Be careful not to let the pipette tip disrupt the axons.</li>
		<li>Leave the culture in the biosafety cabinet for 1 h at room temperature to allow the GBM neurosphere to attach.</li>
		<li>Once the neurosphere has attached, very carefully replace the media in the distal compartment with Supplemented NB Medium.</li>
		<li>Live image cultures for 3-7 days, adding media as needed. In this case, a controlled CO2 live cell enclosure attached to the microscope (e.g., Zeiss Axiovert) was used to continuously monitor cell migration for 7 days using brightfield. Images were acquired every 10 min using the associated software. Repeat the same protocol using hGCs that have been stably transfected to express GFP and images were captured using a combination of brightfield and 488 nm laser. 
		NOTE: Neurospheres may be transduced with lentivirus or transfected with plasmids or siRNAs. Compartments may also be treated with desired small molecule inhibitors to study migration.</li>
	</ol>
	</li>
	<li>Myelination of DRG Axons with Oligodendrocytes
	<ol>
		<li>The day before oligodendrocyte isolation, replace the NB Medium in the DRGs with C-Medium.</li>
		<li>Before dissection, place 10 mL of Papain Buffer into a 60 mm dish in the incubator to equilibrate.</li>
		<li>In a laminar flow hood, fill one 100 mm dish and one 60 mm dish with ice cold HBSS. Place on ice.</li>
		<li>Sacrifice a P2 rat pup by decapitation. Remove the skin using a pair of scissors. After the skin is removed, cut the skull along the midline with a pair of fine scissors.</li>
		<li>Gently remove the skull with fine forceps. Using a spatula, gently scoop the brain from the bottom of the skull and transfer to an inverted tissue culture plate lid.</li>
		<li>Remove the cerebellum and divide the cerebrum into two cerebral hemispheres. Remove the olfactory bulbs, hippocampus, and basal ganglia below the cerebral cortex of each hemisphere. Place the cerebral cortex in the 100 mm dish containing HBSS. Repeat steps 3.3.4-3.3.6 for the remaining animals.</li>
		<li>Working with one cortex at a time, remove the meninges with fine Dumont forceps. Place all meninges-free cortices into fresh 60 mm dishes with HBSS.</li>
		<li>Dice the cortical tissue into 1 mm3 pieces. Place on ice.</li>
		<li>Place equilibrated Papain Buffer into a 15 mL tube. Add 200 units of papain and 2 mg L-cysteine. Filter sterilize and add 200 &#181;L of sterile DNase I.</li>
		<li>Remove HBSS from the diced brain tissue and replace with Papain Buffer from 3.3.2. Place dish in 37 &#176;C, 5% CO2 incubator for 80 min, gently shaking every 15 min.</li>
		<li>Transfer the digested tissue to a 50 mL tube and add 2 mL of C-medium.</li>
		<li>Triturate with a 5 mL serological pipette to dissociate the tissue; allow larger pieces of tissue to settle. Remove supernatant and place into a sterile 15 mL tube.</li>
		<li>Add 2 mL of C-Medium and repeat trituration with a 5 mL serological pipette once more. Switch to a 1 mL pipette tip and triturate until the tissue is completely dissociated. Transfer to the 15 mL tube.</li>
		<li>Spin the triturated tissue at 300 x g for 15 min. Carefully remove supernatant and resuspend pellet in 8 mL of DMEM-ITS-G Medium.</li>
		<li>Pre-wet a sterile 30 &#181;M cell strainer with 2 mL of PB Buffer. Place cell strainer over a 50 mL tube and filter the cell suspension 1 mL at a time. Rinse the filter with 5 mL of PB Buffer.</li>
		<li>Transfer the cell suspension to a 100 mm bacteriological plate; incubate for 15 min in a 37 &#176;C, 5% CO2 incubator to allow microglia to attach.</li>
		<li>Remove media and place into a 15 mL tube. Rinse the plate gently with 2 mL of DMEM-ITS-G medium and transfer to the 15 mL tube.</li>
		<li>Resuspend the cell suspension gently. Take 10 &#181;L of the cell suspension and dilute with 10 &#181;l of trypan blue. Count 10 &#181;l of the cell suspension using an automated cell counter or hemocytometer.</li>
		<li>Spin the cell suspension at 300 x g for 10 min.</li>
		<li>Aspirate supernatant completely and resuspend cells in 70 &#181;L of PB Buffer for every 1 x 107 cells. Mix well and incubate at 4 &#176;C for 10 min.</li>
		<li>Add 20 &#181;l of anti-A2B5 microbeads for every 1 x 107 cells. Mix well and incubate at 4 &#176;C.</li>
		<li>Add 1-2 mL of PB Buffer for every 1 x 107 cells and centrifuge at 300 x g for 10 min in a 4 &#176;C centrifuge.</li>
		<li>Aspirate supernatant completely. Resuspend up to a total of 108 cells in 500 &#181;L of PB Buffer. Keep on ice.</li>
		<li>Place a magnetic bead column in the magnetic field of a separator.</li>
		<li>Prepare the column by rinsing with 500 &#181;L of PB Buffer. Apply the cell suspension to the column. Discard flow-through in a waste container (this contains un-labeled cells).</li>
		<li>Wash the column with 500 &#181;L of PB Buffer 3 times. Discard flow-through.</li>
		<li>Remove the column from magnetic separator and place in a collection tube.</li>
		<li>Pipette 1 mL of PB Buffer onto the column. Flush the magnetically labeled cells by firmly pushing the plunger into the column.</li>
		<li>Add 4 mL of C-Medium to the cell fraction and spin at 300 x g for 10 min.</li>
		<li>Remove supernatant and resuspend in 5 mL of N2B2 Medium. Take 10 &#181;L of the cell suspension and dilute with 10 &#181;L of trypan blue. Count 10 &#181;L of the cell suspension using an automated cell counter or hemocytometer.</li>
		<li>Plate oligodendrocytes at a concentration of 150,000 cells per compartmented chamber containing a DRG with fully extended axons.</li>
		<li>The following day change the media in the compartmented chamber to N2B2 to allow for myelination. Replace media every 2-3 days. Myelination will be complete in 14 days.</li>
		<li>On day 14, seed culture with a GBM neurosphere as in 3.2.26-3.2.32. Maintain myelinated cultures in N2B2 Medium for the duration of any experiments. 
		NOTE: The protocol is presented as a flow chart schematic in Figure 1.</li>
	</ol>
	</li>
</ol>

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P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinctive+properties+of+human+adult+brain-derived+myelin+progenitor+cells.">Distinctive properties of human adult brain-derived myelin progenitor cells.</a> American Journal of Pathology. 165 (6), 2167-2175 (2004).</li><li>Zepecki, J. P., Snyder, K. M., Moreno, M. M., Fajardo, E., Fiser, A., Ness, J., Sarkar, A., Toms, S. A., Tapinos, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+human+glioma+cell+migration,+tumor+growth,+and+stemness+gene+expression+using+a+Lck+targeted+inhibitor.">Regulation of human glioma cell migration, tumor growth, and stemness gene expression using a Lck targeted inhibitor.</a> Oncogene. 38, 1734-1750 (2018).</li><li>Chen, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Boyden+chamber+assay.">Boyden chamber assay.</a> Methods in Molecular Bioliogy. 294, 15-22 (2005).</li><li>Merz, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organotypic+slice+cultures+of+human+glioblastoma+reveal+different+susceptibilities+to+treatments.">Organotypic slice cultures of human glioblastoma reveal different susceptibilities to treatments.</a> Neuro-Oncology. 15 (6), 670-681 (2013).</li><li>Singh, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+human+brain+tumour+initiating+cells.">Identification of human brain tumour initiating cells.</a> Nature. 432 (7015), 396-401 (2004).</li><li>Aubert, M., Badoual, M., Christov, C., Grammaticos, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+for+glioma+cell+migration+on+collagen+and+astrocytes.">A model for glioma cell migration on collagen and astrocytes.</a> Journal of the Royal Society Interface. 5 (18), 75-83 (2008).</li><li>Jia, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+three-dimensional+collagen+scaffolds+on+the+expression+profiles+and+biological+functions+of+glioma+cells.">Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.</a> International Journal of Oncology. 52 (6), 1787-1800 (2018).</li><li>Kaphle, P., Li, Y., Yao, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mechanical+and+pharmacological+regulation+of+glioblastoma+cell+migration+in+3D+matrices.">The mechanical and pharmacological regulation of glioblastoma cell migration in 3D matrices.</a> Journal of Cellular Physiology. 234 (4), 3948-3960 (2019).</li><li>Gritsenko, P., Leenders, W., Friedl, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recapitulating+in+vivo-like+plasticity+of+glioma+cell+invasion+along+blood+vessels+and+in+astrocyte-rich+stroma.">Recapitulating in vivo-like plasticity of glioma cell invasion along blood vessels and in astrocyte-rich stroma.</a> Histochemistry and Cell Biology. 148 (4), 395-406 (2017).</li><li>Gritsenko, P. G., Friedl, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adaptive+adhesion+systems+mediate+glioma+cell+invasion+in+complex+environments.">Adaptive adhesion systems mediate glioma cell invasion in complex environments.</a> Journal of Cell Science. 131 (15), (2018).</li><li>Kaur, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cadherin-11,+a+marker+of+the+mesenchymal+phenotype,+regulates+glioblastoma+cell+migration+and+survival+in+vivo.">Cadherin-11, a marker of the mesenchymal phenotype, regulates glioblastoma cell migration and survival in vivo.</a> Molecular Cancer Research. 10 (3), 293-304 (2012).</li><li>Rao, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mimicking+white+matter+tract+topography+using+core-shell+electrospun+nanofibers+to+examine+migration+of+malignant+brain+tumors.">Mimicking white matter tract topography using core-shell electrospun nanofibers to examine migration of malignant brain tumors.</a> Biomaterials. 34 (21), 5181-5190 (2013).</li><li>Huang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+hydrogel+is+suitable+for+targeted+investigation+of+amoeboid+migration+of+glioma+cells.">Three-dimensional hydrogel is suitable for targeted investigation of amoeboid migration of glioma cells.</a> Molecular Medicine Reports. 17 (1), 250-256 (2018).</li></ol>

The authors have nothing to disclose.

Migration studies for hGCs may be performed by using Boyden chamber systems or scratch assays. However, while these experiments fail to give any information regarding the interactions of tumor cells with other surrounding tissues, the present system can recapitulate GC interactions with myelinated and non-myelinated fibers. Furthermore, to study tumor formation and end-point migration, organotypic slice cultures of the rodent brain or in vivo implantation of glioma cells into the rodent brain or flank have previously bee...

Glioblastoma is one of the most aggressive and lethal tumors of the human brain. The current standard of care includes surgical resection of the tumor followed by radiation1 plus concomitant and adjuvant administration of temozolomide2. Even with this multi-therapeutic approach, tumor recurrence is inevitable3. This is partly due to the extensive migratory nature of the tumor cells, which invade the brain parenchyma creating multiple finger-like projections within the brain4 that make complete resection unlikely.
In recent years, it has become evident that the aggressiveness of glioblastoma is due, in part, to the presence of a population of cancer stem cells within the tumor mass5,6, which exhibit high migratory potential7,8, resistance to chemotherapy and radiation9,10 and the ability to form secondary tumors11. GSCs are capable of recapitulating original polyclonal tumors when xenografted to nude mice5.
Despite the wealth of knowledge regarding the genetic background of glioblastomas, studies on glioma cell (GC) migration are currently hindered by a lack of efficient in vitro or in vivo migration models. Notably, while glioma cell-axonal interactions modulated by cellular and environmental factors are a core component of glioma invasion, to our knowledge there is currently no experimental system with the ability to model these interactions12,13,14. To address this deficiency, we developed an ex vivo culture system of primary hGCs co-cultured with purified DRG axon-oligodendrocytes that results in elevated expression of differentiated tumor markers as well as extensive migration and interaction of hGCs with myelinated and non-myelinated fibers. This ex vivo platform, due to its compartmentalized layout, is suitable for testing the effects of novel therapeutics on hGC migration patterns.,

<table><tbody><tr><td>100 mm Suspension Culture Dish</td><td>Corning</td><td>430591</td><td></td></tr><tr><td>2.5S NGF</td><td>ENVIGO</td><td>B.5025</td><td></td></tr><tr><td>60 mm Suspension Culture Dish</td><td>Corning</td><td>430589</td><td></td></tr><tr><td>ACK Lysing Buffer</td><td>Thermo Fisher</td><td>A1049201</td><td></td></tr><tr><td>Ammonium Hydroxide Solution</td><td>Fisher Scientific</td><td>A669-500</td><td>Concentrated</td></tr><tr><td>Animal-Free Recombinant Human EGF</td><td>Peprotech</td><td>AF-100-15</td><td></td></tr><tr><td>Animal-Free Recombinant Human FGF-basic (154 a.a.)</td><td>Peprotech</td><td>AF-100-18B</td><td></td></tr><tr><td>Anti-A2B5 MicroBeads, human, mouse, rat</td><td>Miltenyi Biotec</td><td>130-093-392</td><td></td></tr><tr><td>Antibiotic-Antimycotic (100X)</td><td>Thermo Fisher</td><td>15240062</td><td></td></tr><tr><td>AutoMACS Rinsing Solution (PBS, pH 7.2)</td><td>Miltenyi Biotec</td><td>130-091-222</td><td></td></tr><tr><td>B27 Supplement</td><td>Thermo Fisher</td><td>17504044</td><td></td></tr><tr><td>B27 Supplement, minus vitamin A</td><td>Thermo Fisher</td><td>12587001</td><td></td></tr><tr><td>Bacteriological Plate</td><td>BD Falcon</td><td>351029</td><td></td></tr><tr><td>Biotin</td><td>Sigma</td><td>B4639</td><td></td></tr><tr><td>BSA</td><td>Sigma</td><td>A9418</td><td></td></tr><tr><td>Campenot Chamber</td><td>Tyler Research</td><td>CAMP-10</td><td></td></tr><tr><td>Cell Culture Dish</td><td>Corning</td><td>430165</td><td>35mm X 10mm</td></tr><tr><td>Cell Strainer</td><td>BD Falcon</td><td>352350</td><td>70 uM, Nylon</td></tr><tr><td>Cell Strainer</td><td>BD Falcon</td><td>352340</td><td>30 uM, Nylon</td></tr><tr><td>Collagenase/Dispase</td><td>Roche</td><td>11097113001</td><td></td></tr><tr><td>Cultrex Rat Collagen I</td><td>Trevigen</td><td>3440-100-01</td><td></td></tr><tr><td>D-Glucose</td><td>Sigma</td><td>G5146</td><td></td></tr><tr><td>DMEM</td><td>Thermo Fisher</td><td>10313021</td><td></td></tr><tr><td>DNase I</td><td>Sigma</td><td>D7291</td><td></td></tr><tr><td>Dow Corning High-Vacuum Grease</td><td>Fisher Scientific</td><td>14-635-5D</td><td></td></tr><tr><td>Dumont #5 Forceps</td><td>Roboz</td><td>RS-5045</td><td></td></tr><tr><td>E16 Timed Pregnant Sprague Dawley Rat</td><td></td><td></td><td></td></tr><tr><td>EBSS</td><td>Sigma</td><td>E7510</td><td></td></tr><tr><td>EGTA</td><td>Sigma</td><td>E3889</td><td></td></tr><tr><td>FBS</td><td>Hyclone</td><td>SH30070.02</td><td></td></tr><tr><td>FUDR</td><td>Sigma</td><td>F0503</td><td></td></tr><tr><td>GlutaMAX Supplement</td><td>Thermo Fisher</td><td>35050061</td><td></td></tr><tr><td>Ham&#039;s F-12 Nutrient Mix</td><td>Thermo Fisher</td><td>11765054</td><td></td></tr><tr><td>HBSS</td><td>Thermo Fisher</td><td>14175095</td><td></td></tr><tr><td>Hemostatic Forceps</td><td>Roboz</td><td>RS-7035</td><td></td></tr><tr><td>Heparin Sodium Salt, 0.2% in PBS</td><td>Stem Cell Technologies</td><td>07980</td><td></td></tr><tr><td>Hypodermic Needle, 18G</td><td>BD</td><td>511097</td><td></td></tr><tr><td>Insulin-Transferrin-Selenium G</td><td>Thermo Fisher</td><td>41400045</td><td></td></tr><tr><td>L-Cysteine</td><td>Sigma</td><td>C7477</td><td></td></tr><tr><td>L-Glutamine</td><td>Thermo Fisher</td><td>25030081</td><td></td></tr><tr><td>Leibovitz&#039;s L-15 Medium</td><td>Thermo Fisher</td><td>11415064</td><td></td></tr><tr><td>MACS BSA Stock Solution</td><td>Miltenyi Biotec</td><td>130-091-376</td><td></td></tr><tr><td>MACS MultiStand</td><td>Miltenyi Biotec</td><td>130-042-303</td><td></td></tr><tr><td>MEM</td><td>Thermo Fisher</td><td>1190081</td><td></td></tr><tr><td>Mg&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt;</td><td>Sigma</td><td>M2643</td><td></td></tr><tr><td>MiniMACS Separator</td><td>Miltenyi Biotec</td><td>130-042-102</td><td></td></tr><tr><td>MS Columns plus tubes</td><td>Miltenyi Biotec</td><td>130-041-301</td><td></td></tr><tr><td>NAC</td><td>Sigma</td><td>A8199</td><td></td></tr><tr><td>NaHCO&lt;sub&gt;3&lt;/sub&gt;</td><td>Sigma</td><td>S5761</td><td></td></tr><tr><td>Neurobasal Medium</td><td>Thermo Fisher</td><td>21103049</td><td></td></tr><tr><td>Neurobasal-A Medium</td><td>Thermo Fisher</td><td>10888022</td><td></td></tr><tr><td>Ordinary forceps</td><td></td><td></td><td></td></tr><tr><td>P2 Sprague Dawley Rat Pups</td><td></td><td></td><td></td></tr><tr><td>Papain</td><td>Worthington</td><td>LS003126</td><td></td></tr><tr><td>Penicillin-Streptomycin</td><td>Thermo Fisher</td><td>15140148</td><td></td></tr><tr><td>Pin Rake</td><td>Tyler Research</td><td>CAMP-PR</td><td></td></tr><tr><td>Progesterone</td><td>Sigma</td><td>P8783</td><td></td></tr><tr><td>StemPro Accutase Cell Dissociation Reagent</td><td>Thermo Fisher</td><td>A1110501</td><td></td></tr><tr><td>Syrine Grease Applicator</td><td>Tyler Research</td><td>CAMP-GLSS</td><td></td></tr><tr><td>Transferrin</td><td>Sigma</td><td>T2036</td><td></td></tr><tr><td>Uridine</td><td>Sigma</td><td>U3003</td><td></td></tr></tbody></table>

real-time monitoring of human glioma cell migration on dorsal root ganglion axon-oligodendrocyte co-cultures

Glioblastoma is one of the most aggressive human cancers due to extensive cellular heterogeneity and the migration properties of hGCs. In order to better understand the molecular mechanisms underlying glioma cell migration, an ability to study the interaction between hGCs and axons within the tumor microenvironment is essential. In order to model this cellular interaction, we developed a mixed culture system consisting of hGCs and dorsal root ganglia (DRG) axon-oligodendrocyte co-cultures. DRG cultures were selected because they can be isolated efficiently and can form the long, extensive projections which are ideal for migration studies of this nature. Purified rat oligodendrocytes were then added on purified rat DRG axons and induced to myelinate. After confirming the formation of compact myelin, hGCs were finally added to the co-culture and their interactions with DRG axons and oligodendrocytes was monitored in real-time using time-lapse microscopy. Under these conditions, hGCs form tumor-like aggregate structures that express GFAP and Ki67, migrate along both myelinated and non-myelinated axonal tracks and interact with these axons through the formation of pseudopodia. Our ex vivo co-culture system can be used to identify novel cellular and molecular mechanisms of hGC migration and could potentially be used for in vitro drug efficacy testing.

In order to study the interaction of hGCs with axons, we generated purified DRG axons as previously described15,16,17,18. These purified DRG axons were then seeded with hGCs, which formed GFAP+/Ki67+ tumor-like structures integrated within the axonal network, while individual hGCs migrated either in association or between the axons (Figure 2). To determine how hGCs interact wit...

Watch this Scientific Journal Video about Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures at JoVE.com

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

Glioblastoma is one of the most aggressive human cancers due to extensive cellular heterogeneity and the migration properties of hGCs. In order to better ...

real-time-monitoring-human-glioma-cell-migration-on-dorsal-root

Universidad de Cartagena UDEC

Research

JoVE Journal

Cancer Research

6.1K Views.  Brown University, Rhode Island Hospital. Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

Video: Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Migration of cells is a common process that leads to the development and maturation of the vertebrate central nervous system (Hatten, '99). The cerebral cortex consists of two basic neuronal types: excitatory and inhibitory. These cells arise in distinct areas and migrate into the cortex along different routes (Pearlman et al., '98). Inhibitory interneurons migrate tangentially from subcortical sources, mostly from different regions of the ganglionic eminences (Gelman et al., '09; Xu et al., '04). Their movement requires precise spatiotemporal control imposed by environmental cues, to allow for the establishment of proper cytoarchitecture and connectivity in the cerebral cortex (Caviness &amp; Rakic, '78; Hatten, '90; Rakic, '90). To study the migratory behavior of cells generated in proliferative zones of the ganglionic eminences (GE) in newborn ferrets in vitro we used a 3 dimensional culture arrangement in a BD Matrigel Matrix. The culture setup consisted of two GE explants and a source of tested proteins extracted from the cerebral cortex and adsorbed on fluorescent latex Retrobeads IX positioned between the explants (Hasling et al., '03; Riddle et al., '97). After 2-3 days of culture, the cells start to appear at the edge of the explant showing a propensity to leave the tissue in a radial direction. Live imaging allowed observation of migratory patterns without the necessity of labeling or marking the cells. When exposed to fractions of the protein extract obtained from isochronic ferret cortex, the GE cells displayed different behaviors as judged by quantitative kinetic analysis of individual moving cells.

Time lapse imaging of 3D tissue culture allows studying migratory behavior of individual cells originating from ganglionic eminence in reaction to fractionated protein extract from cerebral cortex.

Migration of cells is a common process that leads to the development and maturation of the vertebrate central nervous system (Hatten, '99). The cerebral ...

Neuroscience

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Glioblastoma (GBM) is an aggressive brain tumor that is poorly controlled with the currently available treatment options. Key features of GBMs include rapid proliferation and pervasive invasion into the normal brain. Recurrence is thought to result from the presence of radio- and chemo-resistant brain tumor stem cells (BTSCs) that invade away from the initial cancerous mass and, thus, evade surgical resection. Hence, therapies that target BTSCs and their invasive abilities may improve the otherwise poor prognosis of this disease. Our group and others have successfully established and characterized BTSC cultures from GBM patient samples. These BTSC cultures demonstrate fundamental cancer stem cell properties such as clonogenic self-renewal, multi-lineage differentiation, and tumor initiation in immune-deficient mice. In order to improve on the current therapeutic approaches for GBM, a better understanding of the mechanisms of BTSC migration and invasion is necessary. In GBM, the study of migration and invasion is restricted, in part, due to the limitations of existing techniques which do not fully account for the in vitro growth characteristics of BTSCs grown as neurospheres. Here, we describe rapid and quantitative live-cell imaging assays to study both the migration and invasion properties of BTSCs. The first method described is the BTSC migration assay which measures the migration toward a chemoattractant gradient. The second method described is the BTSC invasion assay which images and quantifies a cellular invasion from neurospheres into a matrix. The assays described here are used for the quantification of BTSC migration and invasion over time and under different treatment conditions.

Here, we describe live-cell imaging techniques to quantitatively measure the migration and invasion of glioblastoma brain tumor stem cells over time and under multiple treatment conditions.

Glioblastoma (GBM) is an aggressive brain tumor that is poorly controlled with the currently available treatment options. Key features of GBMs include ...

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Glioblastomas (GBMs), grade IV malignant gliomas, are one of the deadliest types of human cancer because of their aggressive characteristics. Despite significant advances in the genetics of these tumors, how GBM cells invade the healthy brain parenchyma is not well understood. Notably, it has been shown that GBM cells invade the peritumoral space via different routes; the main interest of this paper is the route along white matter tracts (WMTs). The interactions of tumor cells with the peritumoral nervous cell components are not well characterized. Herein, a method has been described that evaluates the impact of neurons on GBM cell invasion. This paper presents an advanced co-culture in vitro assay that mimics WMT invasion by analyzing the migration of GBM stem-like cells on neurons. The behavior of GBM cells in the presence of neurons is monitored by using an automated tracking procedure with open-source and free-access software. This method is useful for many applications, in particular, for functional and mechanistic studies as well as for analyzing the effects of pharmacological agents that can block GBM cell migration on neurons.

Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.

Glioblastomas (GBMs), grade IV malignant gliomas, are one of the deadliest types of human cancer because of their aggressive characteristics. Despite ...

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Cell migration and invasion are specific hallmarks of Diffuse Midline Glioma (DMG) H3K27M-mutant tumors. We have already modeled these features using three-dimensional (3D) cell-based invasion and migration assays. In this study, we have optimized these 3D assays for live-cell immunocytochemistry. An Antibody Labeling Reagent was used to detect in real-time the expression of the adhesion molecule CD44, on the plasma membrane of migrating and invading cells of a DMG H3K27M primary patient-derived cell line. CD44 is associated with cancer stem cell phenotype and tumor cell migration and invasion and is involved in the direct interactions with the central nervous system (CNS) extracellular matrix. Neurospheres (NS) from the DMG H3K27M cell line were embedded into the basal membrane matrix (BMM) or placed onto a thin coating layer of BMM, in the presence of an anti-CD44 antibody in conjunction with the antibody labeling reagent (ALR). The live-3D-cell immunocytochemistry image analysis was performed on a live-cell analysis instrument to quantitatively measure the overall CD44 expression, specifically on the migrating and invading cells. The method also allows visualizing in real-time the intermittent expression of CD44 on the plasma membrane of migrating and invading cells. Moreover, the assay also provided new insights into the potential role of CD44 in the mesenchymal to amoeboid transition in DMG H3K27M cells.

This study presents a protocol of live-3D-cell immunocytochemistry applied to a pediatric diffuse midline glioma cell line, useful to study in real-time the expression of proteins on the plasma membrane during dynamic processes like 3D cell invasion and migration.

Cell migration and invasion are specific hallmarks of Diffuse Midline Glioma (DMG) H3K27M-mutant tumors. We have already modeled these features using ...

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These neurotropic cancer cells have a unique ability to migrate unidirectionally along nerves towards the central nervous system (CNS). The dorsal root ganglia (DRG)/cancer cell model is a three dimensional (3D) in vitro model frequently used for studying the interaction between neural stroma and cancer cells. In this model, mouse or human cancer cell lines are grown in ECM adjacent to preparations of freshly dissociated cultured DRG. In this article, the DRG isolation protocol from mice, and implantation in petri dishes for co-culturing with pancreatic cancer cells are demonstrated. Five days after implantation, the cancer cells made contact with the DRG neurites. Later, these cells formed bridgeheads to facilitate more extensive polarized, neurotropic migration of cancer cells.

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

This video article shows the use of the dorsal root ganglia (DRG)/cancer cell model in pancreatic ductal adenocarcinoma.

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These ...

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Glioblastoma (GBM) continues to carry an extremely poor clinical prognosis despite surgical, chemotherapeutic, and radiation therapy. Progressive tumor invasion into surrounding brain parenchyma represents an enduring therapeutic challenge. To develop anti-migration therapies for GBM, model systems that provide a physiologically relevant background for controlled experimentation are essential. Here, we present a protocol for generating slice cultures from human GBM tissue obtained during surgical resection. These cultures allow for ex vivo experimentation without passaging through animal xenografts or single cell cultures. Further, we describe the use of time-lapse laser scanning confocal microscopy in conjunction with cell tracking to quantitatively study the migratory behavior of tumor cells and associated response to therapeutics. Slices are reproducibly generated within 90 min of surgical tissue acquisition. Retrovirally-mediated fluorescent cell labeling, confocal imaging, and tumor cell migration analyses are subsequently completed within two weeks of culture. We have successfully used these slice cultures to uncover genetic factors associated with increased migratory behavior in human GBM. Further, we have validated the model&#39;s ability to detect patient-specific variation in response to anti-migration therapies. Moving forward, human GBM slice cultures are an attractive platform for rapid ex vivo assessment of tumor sensitivity to therapeutic agents, in order to advance personalized neuro-oncologic therapy.

Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.

Glioblastoma (GBM) continues to carry an extremely poor clinical prognosis despite surgical, chemotherapeutic, and radiation therapy. Progressive tumor ...

Medicine

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Understanding the mechanisms that control critical biological events of neural cell populations, such as proliferation, differentiation, or cell fate decisions, will be crucial to design therapeutic strategies for many diseases affecting the nervous system. Current methods to track cell populations rely on their final outcomes in still images and they generally fail to provide sufficient temporal resolution to identify behavioral features in single cells. Moreover, variations in cell death, behavioral heterogeneity within a cell population, dilution, spreading, or the low efficiency of the markers used to analyze cells are all important handicaps that will lead to incomplete or incorrect read-outs of the results. Conversely, performing live imaging and single cell tracking under appropriate conditions represents a powerful tool to monitor each of these events. Here, a time-lapse video-microscopy protocol, followed by post-processing, is described to track neural populations with single cell resolution, employing specific software. The methods described enable researchers to address essential questions regarding the cell biology and lineage progression of distinct neural populations.

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

Understanding the mechanisms that control critical biological events of neural cell populations, such as proliferation, differentiation, or cell fate ...

Establishing a Three-dimensional Co-culture Model of Murine Dorsal Root Ganglia and Cancer Cells: An In Vitro Model to Study Perineural Cancer Cell Invasion

Source: Huyett, P. et al. <a href="https://jove.unicartagenaproxy.elogim.com/video/55043" target="_blank">A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma.</a> J. Vis. Exp. (2017)
In this video, we demonstrate the procedure for developing an in vitro perineural cancer cell invasion model by co-culturing mouse dorsal root ganglion and neurotropic cancer cells. The model can be utilized to conduct cancer assays, producing rapid and reproducible results.

Source: Huyett, P. et al. A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma. J. Vis. Exp. (2017)
In this video, we demonstrate the ...

JoVE Encyclopedia of Experiments

Head and Neck Cancer

Assays & techniques

Co-Culturing Glioblastoma Cells on Patterned Neurons: A Technique to Monitor the Migration of Glioblastoma Cells on Rat Hippocampal Neurons In Vitro

Source: Guyon, J. et al. <a href="https://jove.unicartagenaproxy.elogim.com/video/62213" target="_blank">Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions.</a> J. Vis. Exp. (2021)
This video describes a co-culture assay of glioblastoma cells on micropatterned neurons, which mimic the myelinated neuronal tracts invasion by the glioblastoma stem-like cells as they migrate on neurons in the peritumoral spaces. This assay can help analyze the effects of pharmacological drugs that inhibit glioblastoma cells&#39; migration on neurons in a glioma.

Source: Guyon, J. et al. Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions. J. Vis. Exp. 
...

Cancers of the Nervous System

In vitro studies

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture

Source: Zepecki, J. P., et al. <a href="https://appjove.unicartagenaproxy.elogim.com/v/59744">Real-Time monitoring of human glioma cell migration on dorsal root ganglion axon-oligodendrocyte co-cultures.</a> J. Vis. Exp. (2019)
This video demonstrates constructing a three-compartment cell culture system using collagen and non-reactive grease. This system is used to observe interactions between glioma cells and axons in real-time.


	Compartmented culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs), and hGCs (human glioma cells)

1. Preparation of compartmented culture ...

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Summary

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Chapters in this video

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Establishing a Three-dimensional Co-culture Model of Murine Dorsal Root Ganglia and Cancer Cells: An In Vitro Model to Study Perineural Cancer Cell Invasion

Co-Culturing Glioblastoma Cells on Patterned Neurons: A Technique to Monitor the Migration of Glioblastoma Cells on Rat Hippocampal Neurons In Vitro

Preparation of Collagen-Coated Compartmented Culture Dishes for Neuronal Cell Culture