SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10.8K Views
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10:58 min
•
August 24th, 2016
DOI :
August 24th, 2016
•1Department of Cellular and Molecular Physiology, Yale University School of Medicine, 2Nanobiology Institute, Yale University, 3Department of Molecular Biophysics and Biochemistry, Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)
Chapters in this video
0:05
Title
1:01
Preparation of a PDMS Block to Form the Microfluidic Channel
3:17
Formation of the Tethered Supported Bilayer
6:18
Checking Bilayer Fluidity
7:06
Introducing v-SUVs into the Microfluidic Channels
7:59
Observing the Fusion of Single v-SUVs and the SBL
8:42
Results: Docking and Fusion Events Described by Lipid and Content Release
10:26
Conclusion
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