Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
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08:55 min
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February 17th, 2023
DOI :
February 17th, 2023
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Chapters in this video
0:05
Introduction
0:43
Preparation of Lipids and Agarose Hydrogel
1:33
PMMA Chamber Assembly and Lipid Monolayer Formation
3:06
Preparation of Lipid-Coated Aqueous Droplets in Hexadecane/Silicone Oil Solution
4:17
Preparation and Imaging of Single Ion Channels in DIB Membranes
6:49
Results: Isolation of TOM-CC and Correlation of Channel Activity with Lateral Mobility of TOM-CC
8:18
Conclusion
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