Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
7.9K Views
•
11:06 min
•
March 21st, 2017
DOI :
March 21st, 2017
•Chapters in this video
0:05
Title
1:26
Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
3:16
Lys-GFP BMT and Intravital Imaging Preparation
5:23
In Vivo High Resolution Mouse Calvarium Imaging
7:15
Quantitative Analysis
8:14
Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
10:10
Conclusion
Related Videos
09:31
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
11.5K Views
09:02
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
16.7K Views
10:03
Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging
11.6K Views
10:11
Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
15.3K Views
11:27
Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
9.3K Views
12:08
Tracking Mouse Bone Marrow Monocytes In Vivo
9.5K Views
08:41
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
8.1K Views
17:08
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
13.1K Views
12:54
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
13.8K Views
06:28
Simplified Intrafemoral Injections Using Live Mice Allow for Continuous Bone Marrow Analysis
1.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved