The authors acknowledge support from a Translational Breast Cancer Research Grant (14-60-26-BROC to AB) from the Breast Cancer Research Foundation and the American Association for Cancer Research.

hayvan denekleri kullanarak Prosedürleri Texas at Austin Üniversitesi Kurumsal Hayvan Bakım ve Kullanım Kurulu tarafından onaylanmıştır. 1. Ameliyat öncesi hazırlık <ol><li> Her tavşan vücut ağırlığını kaydedin. tüm preklinik çalışmalarda olduğu gibi, potansiyel toksisite değerlendirmek için düzenli olarak hayvan ağırlıkları izlemek. </li><li> Önce tavşan anestezi için, piyasada mevcut ultrason jeli ve 30 saniye süreyle 500 xg&#39;de spin ile 50 ml konik tüp doldurmak; Santrifüj işlemi tamamlandıktan sonra jeli içinde hiçbir görünür kabarcıklar olmalıdır. </li><li> 0.75 mg / kg bir dozda asepromazin kas içine 0.1 mg / kg&#39;lık bir dozda subkutan olarak glikopirolat yönetme ve. vital bulguları ve davranışlarını izlerken etkili olması sedatif için 15-20 dakika bekleyin. NOT: Glikopirolat bradikardi önler ve solunum ve sindirim salgıları azaltan bir anti-kolinerjik ajandır. Acepromazine bir premedicati olarak hizmet veren bir yatıştırıcıanestezi için üzerinde. </li><li> ketamin 35 mg / kg ve kg xylazine deri altından bir anestezik olarak 5 mg / yönetmek. Bununla birlikte, operatör daha deneyimli hale gelir ve ketamin ve 3 mg / kg, 15 mg ilaç dozajları azaltmak daha hızlı intraduktal teslim yerine gibi / ksilazin subkutan kg; Bu anestezi süresini ve anestezi kurtarmak için hayvan için gereken süreyi kısaltır. </li><li> Kontrol ve kalp hızı, DPT 2, sıcaklık, solunum hızı ve mukus zarının rengi her 15 dk belgelemek. her iki gözde göz yağ sürün. NOT: Uygun eğitim geçirmiş ve yönetmek veya anestezi takip etmelidir onların kurumun IACUC tarafından onaylanmıştır Sadece personel. Bir veteriner anestezi monitör kullanımı vital bulguları elde etmek ve tavsiye edilir yardımcı olabilir. veteriner anestezi monitör, ancak, elle hayvana her 15 dk kontrol etme ihtiyacı yerine geçmez. f üreticinin talimatlarına bakınveya bir veteriner monitörün doğru kullanımı. </li><li> hafif bir ayak tutam anestezi başlangıcını doğrulama; Tavşan devam etmeden önce olmayan duyarlı olmalıdır. </li><li> Dikkatle kasık emzikleri üçüncü ve dördüncü çiftler etrafındaki alanda tavşan kaudal karın tıraş. </li><li> kaldırıldı saç çoğunluğu ile, traş alana bir over-the-counter epilasyon kremi uygulayın. Ilık su ile ıslatılmış nemli kağıt havlu kullanarak uygulamadan sonra krem ​​10 dakika çıkarın. NOT: Tavşan cilt hassas / çok kırılgan olduğunu. tüy dökücü krem ​​en fazla 10 dakika süreyle kullanılmalıdır. Aslında, 5 dakika bir &quot;test nokta&quot; denemek ve test nokta süresi gereklidir uzun bir zamanı gösterir eğer sadece uzun bırakmak için güvenlidir. </li><li> enjeksiyon bölgesini temizlemek için alkol emdirilmiş gazlı bez ile bölgeyi silin. </li><li> Bir çevrimli ılık su örtüsü ve emici bir ped ile kaplı bir V-şekilli oluğu içinde de sırtlarında tavşan yerleştirin. </li></ol> 2. PrKontrast maddenin Ayırma <ol><li> üreticinin talimatlarına göre hedeflenmemiş kontrast reaktif sulandırın. yukarı pipet ve aşağı hafifçe karıştırın. NOT: Bu protokolde kullanılan kontrast maddesi 4, oda sıcaklığında stabil olan - sulandırıldıktan sonra 6 saat. Yavaşça her alma arasındaki flakon sallayın. Not: kanallarının sayısına bağlıdır gereken solüsyon hacmi enjekte edilecek. Çözelti bir yetişkin Yeni Zelanda Beyaz tavşan (Oryctolaguscuniculus) bir duktal ağacı doldurmaya yetecek kadar, tavşan meme başına 4 duktal açıklıklar ve 0.2 mL vardır. Bu nedenle, 0.8 mL bir toplam hacim, bir meme bezi ile verilebilir. </li></ol> 3. İntraduktal Teslimat <ol><li> enjekte edilecek, uygun emzikleri yerini; hayvanın sırtında konumlandırılmış zaman kolayca görülebilir şekilde kasık başlıkları 3. ve 4. çifti, tavsiye edilir. </li><li> steril% 0.9 tuzlu su yük 0.2 mL22 G iğne ile 1 ml Luer-lock tüberkülin şırınga. Düzgün tuzlu şırınga bir kez 22 G iğne imha ve steril bir 25-gauge iğne ile değiştirin. Yavaşça bir gazlı bez üzerinde% 85 izopropil alkol ile silin. </li><li> İğne yukarı ve hayvan vücuduna şırınga paralel eğim ile, meme tarafına iğne konik yerleştirin ve yavaş tuz 0.1-0.2 mL enjekte; Bu duktal açıklıkların daha iyi görünüm için izin verecektir. </li><li> Yük 1 ml Luer-kilit tüberkülin şırıngaya enjeksiyon solüsyonu 0.2 mL. </li><li> başparmak ve işaret parmağı ile hafifçe emzik tutun ve intraduktal enjeksiyon için konumlandırmak için hafifçe kaldırın; Bir giyilebilir göz büyüteç duktal açıklıklar görselleştirmek yardımcı olabilir. </li><li> meme kaldırdı konumunu korurken, dikkatli bir 25 G ucu künt iğne kullanarak ilgi kanalı kanüle. </li><li> kanülasyon sonra, yavaşça künt-t hub Luer-lock şırınga bükümyerine oturana kadar ip infüzyon iğne. </li><li> emzik kaldırın ve hızla kanal içinde sıvı hareket ettirerek kaynaklanan potansiyel zararı en aza indirmek için yavaş yavaş çözüm enjekte; solüsyon enjekte zaman hiçbir zaman direnç olmalıdır. </li></ol> 4. Ultrasonik Görüntüleme <ol><li> ilgi alanı cilde santrifüj ultrason jeli bol miktarda uygulayın. Bu görüntü kalitesini tehlikeye gibi, jel hiçbir baloncuklar olmadığından emin olun. </li><li> 6 mm görüntüleme derinliğini ayarlayın. jeli ile temas halinde 21 MHz transdüser yerleştirin ve B-mod ilgi alanı tarayın. duktal açma ve kanal boyunca da dahil olmak üzere, taranan bölgede kontrast madde gözlemleyin. NOT: Bu ayarlar, belirli bir foto akustik ultrason makinesi ile kullanılmak üzere geliştirilmiştir. Daha fazla bilgi için Malzeme Tablo bakın. Meme optimize etmek için iletim gücü ve diğer görüntüleme sistemlerinin görüntüleme derinliğini ayarlamak için gerekli olabilirbezi görselleştirme. </li><li> Bir gazlı bezle hayvanın derisinden ultrason jeli çıkarın. </li></ol> 5. Ameliyat Sonrası Bakım <ol><li> meme bölgeye veya çevredeki dokuya travma izi yok olmalı, ve emzik çevredeki şişlik olasılıkla meme yağ pedi enjeksiyon yerine başarılı bir intraduktal enjeksiyon gösterir: enjeksiyon bölgesini gözlemlemek. </li><li> Bir sternum konumda tavşan yerleştirin. Gerekirse, intravenöz marjinal kulak damarına yohimbin 0.2 mg / kg verilmesi; Bu ksilizin etkisini tersine çevirmek ve hayvan anestezi daha hızlı kurtarmak için izin verecek. </li><li> hayvan duyarlı, uyanık olduğunu ve sternum konumunu korumaktadır kadar tavşan iyileşme süresi boyunca her 15 dakikada izleyin. Not: Bu yordam doku hasarı ya da şişlik neden olmamalıdır. kızarıklık veya şişme gözlenir Eğer hayvan uyarı ve güçlü bir kez, meloksikam 0.1-0.2 mg / kg PO bir doz yönetmekağızdan ilaç almak. Daha fazla yardım için kurumun veteriner ekibine başvurun. </li></ol>

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The authors have nothing to disclose.

Tavşan meme bezi ile intraduktal verme metodu, vital boyaların ve terapötik içeren ultrason kontrast reaktifler ve diğer sulu çözeltiler için kullanılabilir. Daha önceki çalışmalar, hormonlar 17, 18, 19 intraduktal teslim göstermiştir. Kemirgen modellerinde, nükleik asitler, 8 intraduktal teslimat, kemoterapötikler 6, 7, ve nanop...

<html> Terapötik maddelerin intraduktal dağıtım kemirgen modellerinde ve erken evre insan denemelerinde 3, 4, 5, 6, 11, 12 olarak ele alınmıştır. Eğitim Bir son Faz intraduktal karboplatin veya invaziv kanser 2 tedavisi için mastektomi bekleyen kadınların intraduktal pegile lipozomal doksorubisin güvenliğini ve uygulanabilirliğini göstermiştir. Intraduktal teslimat için mi protokoller, fare ve sıçan meme bezleri 6, 7, 8, 9 için geliştirilmiştir. araştırma amaçlı, intraduktal tümör hücre enjeksiyonları ve onkogenler lentiviral vektör dağıtımı için de kemirgen modellerinde yapılmıştırref &quot;&gt; 13, 14, 15, 16. Bununla birlikte, intraduktal dağıtım işleminin in vivo model bir yere tedavi bileşiklerin yeni sınıfları gelişimine olanak ve klinik öncesi değerlendirilmesini kolaylaştıracaktır. Kemirgenlerde ve insanlardaki arasındaki anatomik farklılıklara bu çeviri karmaşık bir hale getirmiştir çalışmaları. Farelerde farklı olarak, her kanalı ayrı bir memesinden sona erer, insan meme 5 ila 9, bağımsız duktal sistemleri, meme sonlanan ayrı açıklık ile, her oluşur. Tavşan meme bezleri dört bağımsız duktal sistemleri, tek bir meme dört menfez biri aracılığıyla ayrı ayrı erişilebilir liman. Bir tavşan modeli daha yakından insan anatomisi ile eşleşir ve daha alakalı bir bağlamda intraduktal ilaç dağıtım çalışmasına izin verir. Burada, intraduktal teslim değerlendirmek için iki tekniklerini kullanın. Bir eş zamanlı uygulanmasıvital boya deri yoluyla görselleştirme izin verir ve yöntemin basit ve hızlı onay sağlar. Bazı uygulamalar için, kanalların yüksek çözünürlük eşleme tercih edilebilir. Biz olmayan bir hedefli kontrast reaktif intraduktal teslim yoluyla burada kanalların ultrason görüntüleme için bir protokol mevcut.

<table><tbody><tr><td>MicroMarker non-targeted contrast reagent</td><td>VisualSonics</td><td>VS-11694</td><td></td></tr><tr><td>Luer Lock 1 mL Syringes</td><td>BD</td><td>309628</td><td></td></tr><tr><td>Glycopyrrolate 0.2 mg/mL</td><td>Wedgewood Compounding Pharmacy</td><td>GLYCOP-INJ013VC</td><td>6 month shelf life, supply may be limited.&amp;nbsp;</td></tr><tr><td>Atropine Sulfate 0.5 mg/mL</td><td>Animal Health International</td><td>15320764</td><td>If glycopyrrolate is unavailable. Not to be combined with glycopyrrolate.</td></tr><tr><td>Ketamine HCL 100 mg/mL</td><td>Animal Health International</td><td>21250699</td><td>&lt;a href=&quot;http://www.animalhealthinternational.com/&quot;&gt;http://www.animalhealthinternational.com/&lt;/a&gt;</td></tr><tr><td>Acepromazine 10 mg/mL</td><td>Animal Health International</td><td>17640541</td><td></td></tr><tr><td>Xylazine 20 mg/mL</td><td>Animal Health International</td><td>20101547</td><td></td></tr><tr><td>Yohimbine 0.2 mg/mL</td><td>Animal Health International</td><td>14588965</td><td></td></tr><tr><td>Hair Removing Cream</td><td>Veet</td><td></td><td>Sensitive skin solution. Available through local retailers.</td></tr><tr><td>Blunt tip infusion needles</td><td>Sai Infusion Technology</td><td>B14-50</td><td>&lt;a href=&quot;http://www.sai-infusion.com/collections/blunt-needles&quot;&gt;http://www.sai-infusion.com/collections/blunt-needles&lt;/a&gt;</td></tr><tr><td>Veterinary Pulse Oximeter</td><td>EdanUSA</td><td>VE-H100B</td><td>&lt;a href=&quot;http://www.edanusa.com/Product/VE-H100B-Veterinary-Pulse-Oximeter.html&quot;&gt;http://www.edanusa.com/Product/VE-H100B-Veterinary-Pulse-Oximeter.html&lt;/a&gt;</td></tr><tr><td>Warm Water Pump</td><td>Gaymar</td><td>TP700</td><td></td></tr><tr><td>Warm Water Blanket</td><td>Animal Health International</td><td>21232696</td><td>Maxi-Therm Lite Warming Pads</td></tr><tr><td>Ultrasound system</td><td>VisualSonics</td><td>Vevo 2100</td><td></td></tr></tbody></table>

intraductal delivery to the rabbit mammary gland

Göğüs kanseri için lokalize intraduktal tedavi tümör verimli verme ve düşük sistemik toksisite ve yan etkileri, 1, 2, 3, 4, 5, 6, 7 de dahil olmak üzere, potansiyel avantajlar sunar. Bu tedavilerin daha yaygın olarak uygulanabilir önce Ancak, bazı zorluklar devam etmektedir. Uygun bir hayvan modelinde geliştirilmesi ve intraduktal tedavilerin doğrulama hastalar için intraduktal tedavi stratejilerinin geliştirilmesini kolaylaştırır. fare meme bezi geniş meme gelişimi ve kanser oluşumunda bir model sistem olarak kullanılmış olsa da, anatomi insan bezi ayrıdır. Daha büyük bir hayvan modeli, örneğin tavşan gibi, meme bezi yapısı ve intraduktal terapötik gelişim için iyi bir model olarak hizmet edebilir. c&#39;defarelere ontrast, hangi on duktal ağaçları uzamsal ayrı bir meme her sonlandırıcı, tavşan meme bezi daha yakından bir emzik ayrı deliklerden bu çıkış Birden fazla örtüşen duktal sistemi ile, insan bezi benzer, vücut ekseni boyunca dağıtılır. Burada, doğrudan tavşan meme kanala ve yüksek çözünürlüklü ultrason görüntüleme ile teslimat kendisi görselleştirme için reaktiflerin teslimat için minimal invaziv yöntemler sunuyoruz.

Burada, bir tavşanın meme kanallara kontrast reaktifler intraduktal dağıtım doku (Şekil 2) bir travma olmaksızın elde edilebileceğini göstermektedir. tavşanlarda, dört ayrı duktal sistemleri bir meme yakınsama ve böylece ulaşılabilir ve bu yöntemi kullanarak bireysel olarak görüntülenmiş. Bireysel duktal açıklıklar kolayca görülebilir; Şekil 2B&#39;de kanül kanalına bitişik olan bir ikinci duktal açıklığı markalama ok ...

Watch this Scientific Journal Video about Intraductal Delivery to the Rabbit Mammary Gland at JoVE.com

Intraductal Delivery to the Rabbit Mammary Gland

Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.

Rabbit Meme Bezi için İntraduktal Teslimat

Localized intraductal treatments for breast cancer offer potential advantages, including efficient delivery to the tumor and reduced systemic toxicity and ...

intraductal-delivery-to-the-rabbit-mammary-gland

Universidad de Cartagena UDEC

Research

JoVE Journal

Bioengineering

9.3K Görüntüleme.  The University of Texas at Austin. Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents. 

Video: Intraductal Delivery to the Rabbit Mammary Gland

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal and postnatal consequences. To ensure the innocuousness of ART applications, studies in animal models are necessary. In addition, as a last step, embryo development studies require evaluation of their capacity to develop full-term healthy offspring. Here, embryo transfer to the uterus is indispensable to perform any ARTs-related experiment.
The rabbit has been used as a model organism to study mammalian reproduction for over a century. In addition to its phylogenetic proximity to the human species and its small size and low maintenance cost, it has important reproductive characteristics such as induced ovulation, a chronology of early embryonic development similar to humans and a short gestation that allow us to study the consequences of ART application easily. Moreover, ARTs (such as intracytoplasmic sperm injection, embryo culture, or cryopreservation) are applied with suitable efficiency in this species.
Using the laparoscopic embryo transfer technique and the cryopreservation protocol presented in this article, we describe 1) how to transfer embryos through an easy, minimally invasive technique and 2) an effective protocol for long-term storage of rabbit embryos to provide time-flexible logistical capacities and the ability to transport the sample. The outcomes obtained after transferring rabbit embryos at different developmental stages indicate that morula is the ideal stage for rabbit embryo recovery and transfer. Thus, an oviductal embryo transfer is required, justifying the surgical procedure. Furthermore, rabbit morulae are successfully vitrified and laparoscopically transferred, proving the effectiveness of the described techniques.

Assisted reproductive techniques (ARTs) are in continuous evaluation to improve outcomes and reduce the associated risks. This manuscript describes a minimally invasive embryo transfer procedure with an efficient cryopreservation protocol that allows the use of rabbits as an ideal animal model of human reproduction.

En düşük Invaziv embriyo transferi ve tavşan modelinde optimum embriyo aşamasında embriyo vitrifikasyon

Assisted reproductive techniques (ARTs), such as in vitro embryo culture or embryo cryopreservation, affect natural development patterns with perinatal ...

Gelişim Biyolojisi

Intraductal Delivery and X-ray Visualization of Ethanol-Based Ablative Solution for Prevention and Local Treatment of Breast Cancer in Mouse Models

Breast cancer is the most prevalent cancer and the second-leading cause of cancer-related death for women in the USA. For high-risk women, prophylactic mastectomy is the most effective primary prevention strategy. Prophylactic mastectomy is an aggressive surgical procedure that completely removes the mammary epithelial cells from which breast cancer arises along with the surrounding tissue. We seek to develop a minimally invasive intraductal procedure as an alternative to prophylactic mastectomy to locally ablate the mammary epithelial cells before they can become malignant. We and others have developed an intraductal delivery procedure to reach and treat these epithelial cells in rodent models of breast cancer. While the mouse mammary gland with a single non-anastomosed ductal tree opening at the nipple has a much less complex and tortuous architecture than the human breast, chemically induced and genetically engineered mouse models of breast cancer are valuable to produce proof-of-concept studies of new preventative strategies. Here, we describe a procedure for intraductal delivery of an ethanol-based ablative solution containing micro-CT/X-ray tantalum-based contrast agent within the mouse mammary ductal tree for the therapeutic purpose of primary prevention of breast cancer. Intraductal delivery of aqueous reagents (e.g., cytotoxic compounds, siRNAs, AdCre) has been previously described in mouse models. Thus, we focus our protocol description on methodological modifications and unique experimental considerations for optimizing delivery of ethanol, for minimizing local and systemic side effects of ethanol administration, and for in vivo visualization of ductal tree filling via micro-CT/fluoroscopy imaging. Visualization of the ductal tree immediately after injection of a contrast-containing solution allows for confirmation of complete filling or unsuccessful outcomes such as underfilling or overfilling. This procedure can be applied for delivery and imaging of other ablative compounds aimed at either preventing tumor formation or locally treating early-stage tumors accessible via the ductal tree.

A method of intraductal injection of reagents for an ethanol-based ablative solution to the mouse mammary ductal tree for in vivo imaging and breast cancer prevention is described. Injection directly into the nipple opening allows for targeting mammary epithelial cells with minimal collateral tissue damage.

Fare Modellerinde Meme Kanserinin Önlenmesi ve Lokal Tedavisi için Etanol Bazlı Ablatif Çözümün İntradüktal Doğumu ve Röntgen Görselleştirmesi

Breast cancer is the most prevalent cancer and the second-leading cause of cancer-related death for women in the USA. For high-risk women, prophylactic ...

Kanser Araştırmaları

X-Ray Visualization of Intraductal Ethanol-Based Ablative Treatment for Prevention of Breast Cancer in Rat Models

There are still a limited number of primary interventions for prevention of breast cancer. For women at a high risk of developing breast cancer, the most effective intervention is prophylactic mastectomy. This is a drastic surgical procedure in which the mammary epithelial cells that can give rise to breast cancer are completely removed along with the surrounding tissue. The goal of this protocol is to demonstrate the feasibility of a minimally invasive intraductal procedure that could become a new primary intervention for breast cancer prevention. This local procedure would preferentially ablate mammary epithelial cells before they can become malignant. Intraductal methods to deliver solutions directly to these epithelial cells in rodent models of breast cancer have been developed at Michigan State University and elsewhere. The rat mammary gland consists of a single ductal tree that has a simpler and more linear architecture compared to the human breast. However, chemically induced rat models of breast cancer offer valuable tools for proof-of-concept studies of new preventive interventions and scalability from mouse models to humans. Here, a procedure for intraductal delivery of an ethanol-based ablative solution containing tantalum oxide nanoparticles as X-ray contrast agent and ethyl cellulose as gelling agent into the rat mammary ductal tree is described. Delivery of aqueous reagents (e.g., cytotoxic compounds, siRNAs, AdCre) by intraductal injection has been described previously in mouse and rat models. This protocol description emphasizes methodological changes and steps that pertain uniquely to delivering an ablative solution, formulation consideration to minimize local and systemic side effects of the ablative solution, and X-ray imaging for in vivo assessment of ductal tree filling. Fluoroscopy and micro-CT techniques enable to determine the success of ablative solution delivery and the extent of ductal tree filling thanks to compatibility with the tantalum-containing contrast agent.

A procedure for the delivery of a chemical ablative solution to the rat mammary ductal tree for image-guided preventive treatment of breast cancer is described. Mammary epithelial cells can be targeted with minimal collateral tissue damage through cannulation directly into the nipple opening and intraductal infusion of a 70% ethanol-based ablative solution.

Sıçan Modellerinde Meme Kanserinin Önlenmesi için İntraduktal Etanol Bazlı Ablatif Tedavinin X-Ray Görselleştirmesi

There are still a limited number of primary interventions for prevention of breast cancer. For women at a high risk of developing breast cancer, the most ...

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Mouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play a major role in mammary gland function and are the origin of most mammary tumors. Introducing genes of interest into mouse mammary epithelial cells is a critical step in evaluating gene function in epithelial cells and generating mouse mammary tumor models. This goal can be accomplished through the intraductal injection of a viral vector carrying the genes of interest into the mouse mammary ductal tree. The injected virus subsequently infects mammary epithelial cells, bringing in the genes of interest. The viral vector can be lentiviral, retroviral, adenoviral, or adenovirus-associated viral (AAV). This study demonstrates how a gene of interest is delivered into mammary epithelial cells through mouse mammary intraductal injection of a viral vector. A lentivirus carrying GFP is used to show stable expression of a delivered gene, and a retrovirus carrying Erbb2 (HER2/Neu) is used to demonstrate oncogene-induced atypical hyperplastic lesions and mammary tumors.

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

The present protocol describes intraductal injection of viral vectors via the teat to deliver genes of interest into the mammary epithelial cells.

İn Vivo Meme İntraduktal Enjeksiyon Yoluyla Fare Meme Epitel Hücrelerine Gen İletimi

Mouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play ...

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits (Oryctolagus cuniculus)

Due to similarities in placentation and antibody transfer with humans, rabbits are an excellent model of maternal immunization. Additional advantages of this research model are the ease of breeding and sample collection, relatively short gestation period, and large litter sizes. Commonly assessed routes of immunization include subcutaneous, intramuscular, intranasal, and intradermal. Nonterminal sample collection for the chronological detection of the immunologic responses to these immunizations include the collection of blood, from both dams and kits, and milk from the lactating does. In this article, we will demonstrate techniques our lab has utilized in studies of maternal immunization in New Zealand White rabbits (Oryctolagus cuniculus), including intranasal immunization and milk collection.

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus

This article describes and demonstrates the administration of intranasal vaccines and the collection of milk from lactating rabbits (Oryctolagus cuniculus) as a means to assess mucosal immunity in a translationally appropriate model of maternal immunization.

Yeni Zelanda Beyaz Tavşanlarında Anne Bağışıklama Çalışmalarında İntranazal Bağışıklama ve Süt Toplama (Oryctolagus cuniculus)

Due to similarities in placentation and antibody transfer with humans, rabbits are an excellent model of maternal immunization. Additional advantages of ...

İmmünoloji ve Enfeksiyon

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve due to the lack of appropriate targeting molecules that are independent of developmental stages such as pregnancy and lactation. Herein, we describe a technique for localized delivery of reagents to the mammary gland at any stage in adulthood via intraductal injection into the nipples of mice. The injections can be performed on live mice, under anesthesia, and allow for a non-invasive and localized drug delivery to the mammary gland. Furthermore, the injections can be repeated over several months without damaging the nipple. Vital dyes such as Evans Blue are very helpful to learn the technique. Upon intraductal injection of the blue dye, the entire ductal tree becomes visible to the eye. Furthermore, fluorescently labeled reagents also allow for visualization and distribution within the mammary gland. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents, and small molecules.

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Fare meme bezi Lokalize İlaç Teslim Intradüktal Enjeksiyon

Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of ...

Biyoloji

Simplifying Transcutaneous Intratracheal Drug Delivery in the Newborn Preterm Rabbit

Intratracheal (IT) drug delivery allows the direct delivery of pharmaceutical substances to the lung, maximizing potential pulmonary benefit and minimizing systemic drug exposure. The transcutaneous technique is simple and allows for the IT delivery of substances to the lung of prematurely born rabbits shortly after birth. Newborn pups are anesthetized with inhaled Isoflurane before being placed in a supine position with the neck extended. The larynx is identified and stabilized before transcutaneous placement of a 26-gauge (G) catheter into the trachea. Following catheterization of the trachea, a 30 G blunt needle attached to a Hamilton syringe is introduced into the IT catheter and is used for delivering a precise volume into the trachea during spontaneous respiration. After the IT injection is completed, the needle and catheter are withdrawn, and the pup is allowed to recover from anesthesia. Transcutaneous IT injection delivers a large proportion of the injected substance to the lung, with the majority remaining in the lung 3 hours after the intervention. The injections are well tolerated from the day of birth and can be repeated for multiple consecutive days without influencing survival. This technique can be used to investigate the effect of pharmaceutical agents on lung development and in the prevention of neonatal lung injury in preterm rabbits.

<meta charset="utf8"></head><body>Yenidoğan Preterm Tavşanda Transkutanöz İntratrakeal İlaç Dağıtımının Basitleştirilmesi

Transcutaneous intratracheal injection allows for effective intrapulmonary drug delivery during spontaneous respiration. Single and multiple injections are well tolerated with no effect on survival. The technique is simple to perform and can examine the effect of substances on lung development and the prevention of lung injury in newborn rabbits.

Yenidoğan Preterm Tavşanda Transkutanöz İntratrakeal İlaç Dağıtımının Basitleştirilmesi

Intratracheal (IT) drug delivery allows the direct delivery of pharmaceutical substances to the lung, maximizing potential pulmonary benefit and ...

Tıp

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits

With the advancement of scientific research, the demand for gene-edited rabbit models is increasing. However, there are limited pregnancy and feeding management systems for gene-edited rabbits, leading to low survival rates among gene-edited rabbits prepared by many inexperienced researchers. Therefore, proper guidance is essential. This article summarizes the pregnancy and feeding practices for genetically modified rabbits developed in the author's laboratory and outlines a set of fundamental processes. These include pregnancy diagnosis, antenatal care, midwifery, assisted breastfeeding, weaning, and other procedures, along with the rescue and care of weak newborn rabbits. Compared to the traditional natural childbirth and nurturing methods used in rabbit farms, this approach involves more refined management, requiring additional time and effort but significantly increasing the survival rate of suckling rabbits. The methods described in this article are suitable for most laboratory breeding scenarios involving gene-edited or embryo-transferred rabbits and provide a straightforward and effective reference for other researchers.

This protocol describes a pregnancy and feeding management technique for embryo-transferred and genetically modified rabbits, aimed at reducing newborn rabbit mortality and enhancing the preparation efficiency of gene-edited rabbits.

Embriyo Transferi Yapılan ve Genetiği Değiştirilmiş Tavşanlarda Gebelik ve Hemşirelik Yönetimi

With the advancement of scientific research, the demand for gene-edited rabbit models is increasing. However, there are limited pregnancy and feeding ...

Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland

Source: Xiang, D., et al. <a href="https://jove.unicartagenaproxy.elogim.com/v/59502/modeling-breast-cancer-via-an-intraductal-injection-cre-expressing">Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland.</a> J. Vis. Exp (2019).
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.

İntraduktal Enjeksiyon: Fare Meme Bezinin Kanallarına Enjeksiyon Karışımının Verilmesi

Source: Xiang, D., et al. Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland. J. Vis. Exp ...

Rabbit Meme Bezi için İntraduktal Teslimat

Özet

Daha Fazla Video Keşfet

Bu videodaki bölümler

En düşük Invaziv embriyo transferi ve tavşan modelinde optimum embriyo aşamasında embriyo vitrifikasyon

Fare Modellerinde Meme Kanserinin Önlenmesi ve Lokal Tedavisi için Etanol Bazlı Ablatif Çözümün İntradüktal Doğumu ve Röntgen Görselleştirmesi

Sıçan Modellerinde Meme Kanserinin Önlenmesi için İntraduktal Etanol Bazlı Ablatif Tedavinin X-Ray Görselleştirmesi

İn Vivo Meme İntraduktal Enjeksiyon Yoluyla Fare Meme Epitel Hücrelerine Gen İletimi

Yeni Zelanda Beyaz Tavşanlarında Anne Bağışıklama Çalışmalarında İntranazal Bağışıklama ve Süt Toplama (Oryctolagus cuniculus)

Fare meme bezi Lokalize İlaç Teslim Intradüktal Enjeksiyon

Yenidoğan Preterm Tavşanda Transkutanöz İntratrakeal İlaç Dağıtımının Basitleştirilmesi

Yenidoğan Preterm Tavşanda Transkutanöz İntratrakeal İlaç Dağıtımının Basitleştirilmesi

Embriyo Transferi Yapılan ve Genetiği Değiştirilmiş Tavşanlarda Gebelik ve Hemşirelik Yönetimi

İntraduktal Enjeksiyon: Fare Meme Bezinin Kanallarına Enjeksiyon Karışımının Verilmesi