Este trabalho foi parcialmente apoiado por KAKENHI (25708020, 15K13812, 15H00860, 15H00986, 16H02081) do JSPS / MEXT Japan, a Asahi Glass Foundation e a iniciativa pré-estratégica da Universidade de Tsukuba, &quot;Ensemble of light with matters and life&quot;.

1. Protocolos de Fabricação de Microsferas de Polímeros <ol><li> Método de difusão de vapor <ol><li> Dissolver 2 mg de polímeros conjugados, tais como P1 (poli [(9,9-dioctilfluoreno-2,7-diil) - alt - (5-octiltieno [3,4- c ] pirrole-4,6-diona-1, 3-diil)]) 28 e P2 (poli [(N- (2-heptildecil) carbazole-2,7-diil) - alt - (4,8-bis [(dodecil) carbonil] benzo [1,2- b : 4,5- b &#39;] ditiofeno-2,6-diil)]) 28 , em 2 mL de clorofórmio (um bom solvente) num frasco de 5 mL. </li><li> Coloque 5 mL de metanol (um solvente pobre) em um frasco de 50 mL. </li><li> Coloque o frasco de 5 mL contendo a solução de clorofórmio do polímero no frasco para injectáveis ​​contendo 50 mL de metanol. </li><li> Coloque o frasco de 50 mL e mantenha-o durante 3 dias a 25 ° C para permitir a precipitação das microesferas de polímero. </li></ol></li><li> Método de mini-emulsão <ol><li> Dissolver 5 mg de polímeros conjugados, tais como polY [9,9-di-n-octilfluorenil-2,7-diil] (PFO) e poli [2-metoxi-5- (3 &#39;, 7&#39;-dimetiloctiloxi) -1,4-fenilenovinileno] (MDMOPPV) Em 1 mL de clorofórmio. </li><li> Dissolver 30 mg (~ 50 mM) de dodecilsulfato de sódio (SDS) em 2 mL de água desionizada. </li><li> Adicionar 100 μL da solução de clorofórmio do polímero a 2 mL de água contendo SDS. </li><li> Agite vigorosamente a mistura de clorofórmio / água usando um homogeneizador de ultra-alta velocidade a 30.000 rpm durante 2 minutos para emulsionar a solução. </li><li> Mantenha-o por 1 dia sem tapar o frasco para evaporar o clorofórmio. </li><li> Centrifugar a dispersão em um tubo de microcentrífuga de 1,5 mL por 5 minutos a 2.200 x g. Remova a solução aquosa sobrenadante contendo SDS. </li><li> Adicione 2 mL de água desionizada e agite vigorosamente. </li><li> Repita o passo 1.2.6 e 1.2.7 três vezes para lavar o SDS residual. </li></ol></li><li> Método de precipitação da interface </li><li> Dissolver 200 μg de poliestireno (PS) e 10 μG de corante fluorescente (boro dipirrina, BODIPY) a 0,2 mL de tetrahidrofurano (THF). </li><li> Leve suavemente a solução de THF em 1 mL da camada de água. </li><li> Mantenha o THF / água separado de duas camadas durante 6 h sem tampar o frasco para permitir a precipitação das microesferas de polímero. </li></ol> 2. Medição de micro-fotoluminescência (μ-PL) <ol><li> Preparação de amostra <ol><li> Diluir uma suspensão das microesferas preparadas na secção 1 num não solvente ( isto é, metanol ou água desionizada). </li><li> Soltou uma gota (20-30 μL) da suspensão diluída das microesferas sobre um substrato de quartzo usando um revestidor de espuma (tipicamente, 2.000 rpm por 50 s). </li><li> Secar ao ar a película fundida resultante até que os solventes tenham evaporado completamente (~ 5 min). </li></ol></li><li> Configuração Experimental <ol><li> Coloque o substrato de quartzo (15 x 15 x 0,5 mm 3 ) no estágio da amostra de um micr ópticoOscope. </li><li> Encontre microesferas bem definidas que sejam isoladas de outras esferas e apropriadas para a medição μ-PL. </li><li> Selecione um laser ( ie, comprimento de onda, onda contínua ou pulso, tempo de irradiação, integração, etc. ). </li><li> Selecione a ampliação da lente. </li></ol></li><li> Medidas <ol><li> Use um feixe laser focado para irradiar a microesfera. Use a seguinte condição laser: laser cw ou pulsado com comprimentos de onda de excitação ( λ ex ) de 405 nm (cw), 450 nm (cw), 355 nm (pulso laser, freqüência, 1 kHz, duração do pulso, 7 ns) e 470 Nm (pulso laser, freqüência, 2,5 MHz, duração do pulso, 70 ps). </li><li> Registre o espectro PL no ponto excitado usando um espectrômetro com uma grade de 300 ou 1.200 ranhuras mm -1 . </li><li> Tire uma imagem fluorescente. </li><li> Mude o ponto de excitação movendo o estágio da amostra. </li><li> Mude o ponto de detecção, inclinando o colimador(se necessário). </li></ol></li></ol> 3. Técnica de Micromanipulação <ol><li> Manipulação de Microsferas <ol><li> Defina um substrato de quartzo sobre o qual as microesferas são imobilizadas no estágio da amostra de um microscópio óptico. </li><li> Encontre uma microesfera bem definida apropriada para a medição μ-PL. </li><li> Coloque uma micro-agulha de plástico em um aparelho de micro-manipulação. </li><li> Mova a micro-agulha usando um joystick controlado pelo computador para pegar uma microesfera. </li><li> Mova a microesfera e conecte-a a outra microesfera. </li><li> Meça o μ-PL da microesfera conectada. </li></ol></li></ol>

<ol>
	<li>Ford, W. T., Chandran, R., Turk, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catalysts+Supported+on+Polymer+Colloids.">Catalysts Supported on Polymer Colloids.</a> Pure Appl. Chem. 60 (3), 395-400 (1988).</li><li>Chen, C. -. W., Chen, M. -. Q., Serizawa, T., Akashi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+Situ+Synthesis+And+the+Catalytic+Properties+of+Platinum+Colloids+on+Polystyrene+Microspheres+with+Surface-Grafted+Poly(N-isopropylacrylamide).">In Situ Synthesis And the Catalytic Properties of Platinum Colloids on Polystyrene Microspheres with Surface-Grafted Poly(N-isopropylacrylamide).</a> Chem. Commun. , 831-832 (1998).</li><li>Suzuki, K., Yumura, T., Mizuguchi, M., Tanaka, Y., Chen, C. -. W., Akashi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Poly(N-isopropylacrylamide)-Grafted+Silica+as+a+Support+of+Platinum+Colloids:+Preparation+Method,+Characterization,+and+Catalytic+Properties+in+Hydrogenation.">Poly(N-isopropylacrylamide)-Grafted Silica as a Support of Platinum Colloids: Preparation Method, Characterization, and Catalytic Properties in Hydrogenation.</a> J. Appl. Polym. Sci. 77, 2678-2684 (2000).</li><li>Zhang, S., Chen, L., Zhou, S., Zhao, D., Wu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facile+Synthesis+of+Hierarchically+Ordered+Porous+Carbon+via+in+Situ+Self-Assembly+of+Colloidal+Polymer+and+Silica+Spheres+and+Its+Use+as+a+Catalyst+Support.">Facile Synthesis of Hierarchically Ordered Porous Carbon via in Situ Self-Assembly of Colloidal Polymer and Silica Spheres and Its Use as a Catalyst Support.</a> Chem. Mater. 22, 3433-3440 (2010).</li><li>Kataoka, K., Harada, A., Nagasaki, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Block+Copolymer+Micelles+for+Drug+Delivery:+Design+Characterization+And+Biological+Significance.">Block Copolymer Micelles for Drug Delivery: Design Characterization And Biological Significance.</a> Adv. Drug Deliv. Rev. 47, 113-131 (2001).</li><li>Otsuka, H., Nagasaki, Y., Kataoka, K. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=EGylated+Nanoparticles+for+Biological+And+Pharmaceutical+Applications.">EGylated Nanoparticles for Biological And Pharmaceutical Applications.</a> Adv. Drug Deliv. Rev. 55, 403-419 (2003).</li><li>Nishiyama, N., Kataoka, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+State,+Achievements,+And+Future+Prospects+of+Polymeric+Micelles+as+Nanocarriers+for+Drug+and+Gene+Delivery.">Current State, Achievements, And Future Prospects of Polymeric Micelles as Nanocarriers for Drug and Gene Delivery.</a> Pharmacol. Ther. 112, 630-648 (2006).</li><li>Velev, O. D., Kaler, E. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+Situ+Assembly+of+Colloidal+Particles+into+Miniaturized+Biosensors.">In Situ Assembly of Colloidal Particles into Miniaturized Biosensors.</a> Langmuir. 15 (11), 3693-3698 (1999).</li><li>Techawanitchai, P., Yamamoto, K., Ebara, M., Aoyagi, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surface+Design+with+Self-Heating+Smart+Polymers+for+On-Off+Switchable+Traps.">Surface Design with Self-Heating Smart Polymers for On-Off Switchable Traps.</a> Sci. Technol. Adv. Mater. 12, 044609 (2011).</li><li>Lange, U., Roznyatovskaya, N. V., Mirsky, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conducting+Polymers+in+Chemical+Sensors+And+Arrays.">Conducting Polymers in Chemical Sensors And Arrays.</a> Anal. Chim. Acta. 614, 1-26 (2008).</li><li>Rajesh, T., Kumar, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+Progress+in+the+Development+of+Nano-Structured+Conducting+Polymers/Nanocomposites+for+Sensor+Applications.">Recent Progress in the Development of Nano-Structured Conducting Polymers/Nanocomposites for Sensor Applications.</a> Sens. Actuators B. 136, 275-286 (2009).</li><li>Wu, C., Szymanski, C., Cain, Z., McNeill, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conjugated+Polymer+Dots+for+Multiphoton+Fluorescence+Imaging.">Conjugated Polymer Dots for Multiphoton Fluorescence Imaging.</a> J. Am. Chem. Soc. 129, 12904-12905 (2007).</li><li>Feng, L., Zhu, C., Yuan, H., Liu, L., Lv, F., Wang, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conjugated+Polymer+Nanoparticles:+Preparation,+Properties,+Functionalization+And+Biological+Applications.">Conjugated Polymer Nanoparticles: Preparation, Properties, Functionalization And Biological Applications.</a> Chem. Soc. Rev. 42, 6620-6634 (2013).</li><li>Pecher, J., Mecking, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoparticles+of+Conjugated+Polymers.">Nanoparticles of Conjugated Polymers.</a> Chem. Rev. 110, 6260-6279 (2010).</li><li>McGehee, M. D., Heeger, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Semiconducting+(Conjugated)+Polymers+as+Materials+for+Solid-State+Lasers.">Semiconducting (Conjugated) Polymers as Materials for Solid-State Lasers.</a> Adv. Mater. 12, 1655-1668 (2000).</li><li>Samuel, I. D. W., Turnbull, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organic+Semiconductor+Lasers.">Organic Semiconductor Lasers.</a> Chem. Rev. 107, 1272-1295 (2007).</li><li>Kuehne, A. J. C., Gather, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organic+Lasers:+Recent+Developments+on+Materials,+Device+Geometries,+and+Fabrication+Techniques.">Organic Lasers: Recent Developments on Materials, Device Geometries, and Fabrication Techniques.</a> Chem. Rev. 116, 12823-12864 (2016).</li><li>Furumi, S., Kanai, T., Sawada, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Widely+Tunable+Lasing+in+a+Colloidal+Crystal+Gel+Film+Permanently+Stabilized+by+an+Ionic+Liquid.">Widely Tunable Lasing in a Colloidal Crystal Gel Film Permanently Stabilized by an Ionic Liquid.</a> Adv. Mater. 23, 3815-3820 (2011).</li><li>Mikosch, A., Ciftci, S., Kuehne, A. J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colloidal+Crystal+Lasers+from+Monodisperse+Conjugated+Polymer+Particles+via+Bottom-Up+Coassembly+in+a+Sol-Gel+Matrix.">Colloidal Crystal Lasers from Monodisperse Conjugated Polymer Particles via Bottom-Up Coassembly in a Sol-Gel Matrix.</a> ACS Nano. 10, 10195-10201 (2016).</li><li>Oraevsky, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whispering-Gallery+Waves.">Whispering-Gallery Waves.</a> Quant Electron. 32 (5), 377-400 (2002).</li><li>Yamamoto, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spherical+Resonators+from+&#960;-Conjugated+Polymers.">Spherical Resonators from &#960;-Conjugated Polymers.</a> Polym. J. 48, 1045-1050 (2016).</li><li>Adachi, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spherical+Assemblies+from+&#960;+-Conjugated+Alternating+Copolymers:+Toward+Optoelectronic+Colloidal+Crystals.">Spherical Assemblies from &#960; -Conjugated Alternating Copolymers: Toward Optoelectronic Colloidal Crystals.</a> J. Am. Chem. Soc. 135, 870-876 (2013).</li><li>Tong, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tetramethylbithiophene+in+&#960;-Conjugated+Alternating+Copolymers+as+an+Effective+Structural+Component+for+the+Formation+of+Spherical+Assemblies.">Tetramethylbithiophene in &#960;-Conjugated Alternating Copolymers as an Effective Structural Component for the Formation of Spherical Assemblies.</a> Polym. Chem. 5, 3583-3587 (2014).</li><li>Tabata, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-Assembled+Conjugated+Polymer+Spheres+as+Fluorescent+Microresonators.">Self-Assembled Conjugated Polymer Spheres as Fluorescent Microresonators.</a> Sci. Rep. 4, 5902 (2014).</li><li>Kushida, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whispering+Gallery+Resonance+from+Self-Assembled+Microspheres+of+Highly+Fluorescent+Isolated+Conjugated+Polymers.">Whispering Gallery Resonance from Self-Assembled Microspheres of Highly Fluorescent Isolated Conjugated Polymers.</a> Macromolecules. 48, 3928-3933 (2015).</li><li>Kushida, S., Braam, D., Lorke, A., Yamamoto, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whispering+Gallery+Mode+Photoemission+From+Self-Assembled+Poly-Paraphenylenevinylene+Microspheres.">Whispering Gallery Mode Photoemission From Self-Assembled Poly-Paraphenylenevinylene Microspheres.</a> AIP Conf. Proc. ICCMSE. 1702, 090046 (2015).</li><li>Braam, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optically+Induced+Mode+Splitting+in+Self-Assembled,+High+Quality-Factor+Conjugated+Polymer+Microcavities.">Optically Induced Mode Splitting in Self-Assembled, High Quality-Factor Conjugated Polymer Microcavities.</a> Sci. Rep. 6, 19635 (2016).</li><li>Kushida, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Conjugated+Polymer+Blend+Microspheres+for+Efficient+Long-Range+Light+Energy+Transfer.">Conjugated Polymer Blend Microspheres for Efficient Long-Range Light Energy Transfer.</a> ACS Nano. 10, 5543-5549 (2016).</li><li>Kushida, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-Assembled+Polycarbazole+Microspheres+as+Single-Component,+White-Colour+Resonant+Photoemitters.">Self-Assembled Polycarbazole Microspheres as Single-Component, White-Colour Resonant Photoemitters.</a> RSC Adv. 6, 52854-52857 (2016).</li><li>Aikyo, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enwrapping+Conjugated+Polymer+Microspheres+with+Graphene+Oxide+Nanosheets.">Enwrapping Conjugated Polymer Microspheres with Graphene Oxide Nanosheets.</a> Chem. Lett. 45, 1024-1026 (2016).</li><li>Landfester, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Semiconducting+Polymer+Nanospheres+in+Aqueous+Dispersion+Prepared+by+a+Miniemulsion+Process.">Semiconducting Polymer Nanospheres in Aqueous Dispersion Prepared by a Miniemulsion Process.</a> Adv. Mater. 14, 651-655 (2002).</li><li>Okada, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Color-Tunable+Resonant+Photoluminescence+and+Cavity-Mediated+Multistep+Energy+Transfer+Cascade.">Color-Tunable Resonant Photoluminescence and Cavity-Mediated Multistep Energy Transfer Cascade.</a> ACS Nano. 10, 7058-7063 (2016).</li><li>Pecher, J., Mecking, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoparticles+of+Conjugated+Polymers.">Nanoparticles of Conjugated Polymers.</a> Chem. Rev. 110, 6260-6279 (2010).</li><li>Gather, M. C., Yun, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-Cell+Biological+Lasers.">Single-Cell Biological Lasers.</a> Nat. Photon. 5, 406-410 (2011).</li><li>Schubert, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lasing+within+Live+Cells+Containing+Intracelluler+Optical+Microresonators+for+Barcode-Type+Cell+Tagging+and+Tracking.">Lasing within Live Cells Containing Intracelluler Optical Microresonators for Barcode-Type Cell Tagging and Tracking.</a> Nano Lett. 15, 5647-5652 (2015).</li></ol>

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The selection of a good solvent and non-solvent is very important for the self-assembly of well-defined microspheres. If the solubility of a polymer is too high, precipitation will not occur. Also, in general, &#960;-conjugated polymers are hydrophobic, so polar non-solvents, such as MeOH, acetonitrile, and acetone, are often used in the vapor diffusion method to minimize the surface energy required to form a spherical shape. The interface precipitation method is often adopted for the preparation of dye-doped polymer mic...

As partículas de nano / micro-tamanho de polímero são amplamente utilizadas para uma variedade de aplicações, incluindo como suporte de catalisador, enchimentos de cromatografia em coluna, agentes de administração de fármaco, sondas fluorescentes para rastreamento celular, mídia ótica e assim por diante 1 , 2 , 3 , 4 , 5 6 , 7 , 8 , 9 . Em particular, os polímeros conjugados com π têm propriedades inerentes luminescentes e de carga que são benéficas para aplicações ópticas, eletrônicas e optoeletrônicas usando esferas de polímero 10 , 11 , 12 , 13 , 14 , especialmente aplicações de laser usando soft orgMateriais anímicos 15 , 16 , 17 . Por exemplo, a integração tridimensional de esferas com diâmetros de vários centenas de nanômetros forma cristais coloidais, que mostram aberturas da banda fotônica com um certo comprimento de onda 18 , 19 . Quando a luz é confinada na estrutura periódica da intersfera, a ação de laser aparece no meio da banda de parada. Por outro lado, quando o tamanho das esferas aumenta para a escala de vários micrômetros, a luz é confinada dentro de uma única microesfera através da reflexão interna total na interface polímero / ar 20 . A propagação da onda de luz na circunferência máxima resulta em interferência, levando ao aparecimento de um modo ressonante com linhas de emissão afiadas e periódicas. Estes modos ópticos são os chamados &quot;modos de galeria de sussurros&quot; (WGMs). O termo &quot;galeria sussurrante&quot; originou-se deA Catedral de São Paulo em Londres, onde as ondas de som se propagam ao longo da circunferência da parede, permitindo que os sussurros sejam ouvidos por uma pessoa do outro lado da galeria. Como o comprimento de onda da luz está na escala submicrométrica, que é muito menor do que as ondas sonoras, uma grande cúpula não é necessária para o WGM da luz: pequena, escala micrométrica, vasos bem definidos, como microesferas, microdiscs , E microcristais, cumprem as condições do WGM. A Equação 1 é uma forma simples da condição de ressonância de WGM 21 : Nπd = lλ (1) Onde n é o índice de refração do ressonador, d é o diâmetro, l é o número inteiro, e λ é o comprimento de onda da luz. A parte esquerda de (1) é o comprimento do caminho óptico através de uma propagação de círculo. Quando o caminho óptico coincide com oMúltiplo inteiro do comprimento de onda, a ressonância ocorre, enquanto no outro comprimento de onda, a onda de luz é diminuída após o arredondamento. Este artigo apresenta vários métodos experimentais para preparar microesferas para ressonadores WGM a partir de polímeros conjugados em solução: difusão de vapor 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , mini-emulsão 31 e precipitação de interface 32 . Cada método possui características únicas; Por exemplo, o método de difusão de vapor proporciona microesferas bem definidas com esfericidade muito alta e superfícies lisas, mas apenas os polímeros de baixa cristalinidade podem formar essas microesferas. Por outro lado, para a mini emulsãoMétodo, vários tipos de polímeros conjugados, incluindo polímeros de alto cristalino, podem formar esferas, mas a morfologia da superfície é inferior à obtida a partir do método de difusão de vapor. O método de precipitação de interface é preferível para a criação de microesferas a partir de polímeros não conjugados dopados com corantes. Em todos os casos, a seleção do solvente e do não solvente desempenha um papel importante na formação da morfologia esférica. Na segunda metade deste artigo, são apresentadas técnicas de μ-PL e micro-manipulação. Para a técnica μ-PL, as microesferas são dispersas em um substrato, e um feixe laser focado, através de uma lente de microscópio, é usado para irradiar uma única microesfera isolada 24 . O PL gerado de uma esfera é detectado por um espectrômetro através da lente do microscópio. Mover o estágio da amostra pode variar a posição do ponto de excitação. O ponto de detecção também é variável pela inclinação da optica colimadora da excEm relação ao eixo óptico do percurso de detecção 28 , 32 . Para investigar a propagação da luz interesternal e a conversão do comprimento de onda, a técnica de micro-manipulação pode ser usada 32 . Para conectar várias microesferas com diferentes propriedades ópticas, é possível pegar uma esfera usando uma micro-agulha e colocá-la em outra esfera. Em conjunto com as técnicas de micromanipulação e o método μ-PL, várias medições ópticas podem ser realizadas utilizando esferas de polímero conjugadas, que são preparadas por um simples método de auto-montagem. Este documento de vídeo será útil para os leitores que desejam usar materiais poliméricos macios para aplicações ópticas.

<table><tbody><tr><td>polystyrene</td><td>Aldrich</td><td>132427-25G</td><td></td></tr><tr><td>sodium dodecylsulfate</td><td>Kanto Kagaku</td><td>372035-31</td><td></td></tr><tr><td>tetrahydrofuran</td><td>Wako</td><td>206-08744</td><td></td></tr><tr><td>chloroform</td><td>Wako</td><td>038-18495</td><td></td></tr><tr><td>methanol</td><td>Wako</td><td>139-13995</td><td></td></tr><tr><td>Poly(9,9-di-n-octylfluorenyl-2,7-diyl)</td><td>Aldrich</td><td>571652-500MG</td><td></td></tr><tr><td>Poly[2-methoxy-5-(3&amp;prime;,7&amp;prime;-dimethyloctyloxy)-1,4-phenylenevinylene] (MDMOPPV)</td><td>Aldrich</td><td>546461-1G</td><td></td></tr><tr><td>poly[(9,9-dioctylfluorene-2,7-diyl)-alt-(5-octylthieno[3,4-c]pyrrole-4,6-dione-1,3-diyl)] (P1)</td><td>synthesized</td><td>-</td><td>reference 28</td></tr><tr><td>poly[(N-(2-heptylundecyl)carbazole-2,7-diyl)-alt-(4,8-bis[(dodecyl)carbonyl]benzo[1,2-b:4,5-b&amp;prime;]dithiophene-2,6-diyl)] (P2)</td><td>synthesized</td><td>-</td><td>reference 28</td></tr><tr><td>fluorescent dye (boron dipyrrin; BODIPY)</td><td>synthesized</td><td>-</td><td>reference 32</td></tr><tr><td>Optical Microscope</td><td>Nicon</td><td>Eclipse LV-N</td><td></td></tr><tr><td>laser_405 nm</td><td>Hutech</td><td>DH405-10-5</td><td></td></tr><tr><td>Spectrometer</td><td>Lambda Vision</td><td>LV-MC3/T</td><td></td></tr><tr><td>Homogenizer</td><td>Microtech Nichion</td><td>Physcotron NS-360D</td><td></td></tr><tr><td>micromanipulation</td><td>Microsupport</td><td>Quick Pro QP-3RH</td><td></td></tr></tbody></table>

fabrication of polymer microspheres for optical resonator and laser applications

Este artigo descreve três métodos de preparação de microesferas fluorescentes compreendendo polímeros conjugados ou não conjugados π: difusão de vapor, precipitação de interface e mini-emulsão. Em todos os métodos, as esferas bem definidas, de tamanho micrométrico, são obtidas a partir de um processo de auto-montagem em solução. O método de difusão de vapor pode resultar em esferas com maior esfericidade e suavidade da superfície, mas os tipos de polímeros capazes de formar essas esferas são limitados. Por outro lado, no método de mini emulsão, as microesferas podem ser feitas a partir de vários tipos de polímeros, mesmo de polímeros altamente cristalinos com backbones coplanares, π conjugados. As propriedades fotoluminescentes (PL) a partir de microesferas isoladas isoladas são incomuns: a PL é confinada dentro das esferas, se propaga na circunferência das esferas através da reflexão interna total na interface polímero / ar, e auto-interfere para mostrar ressonância afiada e periódica Linhas PL. Essa ressonânciaOs modos g são os chamados &quot;modos de galeria de sussurros&quot; (WGMs). Este trabalho demonstra como medir WGM PL a partir de esferas isoladas isoladas usando a técnica de micro-fotoluminescência (μ-PL). Nesta técnica, um raio laser focado irradia uma única microesfera, e a luminescência é detectada por um espectrômetro. Uma técnica de micromanipulação é então usada para conectar as microesferas uma a uma e para demonstrar a propagação de PL e a conversão de cores entre microesferas acopladas após excitação no perímetro de uma esfera e detecção de PL a partir da outra microesfera. Estas técnicas, μ-PL e micromanipulação, são úteis para experiências em aplicações de micro-ótica usando materiais poliméricos.

A Figura 1 mostra representações esquemáticas do método de difusão de vapor (a), método de mini-emulsão (b) e método de precipitação de interface (c). Para o método de difusão de vapor ( Figura 1a ), colocou-se um frasco de 5 mL contendo uma solução de polímeros de CHCl3 (0,5 mg mL -1 , 2 mL) num frasco de 50 mL contendo 5 mL de um não solvente, tal como MeOH . O frasco exterior foi tampado e depois de...

Watch this Scientific Journal Video about Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications at JoVE.com

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

São apresentados protocolos para a síntese de microesferas a partir de polímeros, manipulação de microesferas e medições de micro-fotoluminescência.

Fabricação de Microsferas de Polímeros para Resonadores Ópticos e Aplicações Laser

This paper describes three methods of preparing fluorescent microspheres comprising &#960;-conjugated or non-conjugated polymers: vapor diffusion, ...

fabrication-polymer-microspheres-for-optical-resonator-laser

Universidad de Cartagena UDEC

Research

JoVE Journal

Engineering

14.0K visualizações.  University of Tsukuba.  São apresentados protocolos para a síntese de microesferas a partir de polímeros, manipulação de microesferas e medições de micro-fotoluminescência. 

Vídeo: Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Fabrication of Silica Ultra High Quality Factor Microresonators

Whispering gallery resonant cavities confine light in circular orbits at their periphery.1-2 The photon storage lifetime in the cavity, quantified by the quality factor (Q) of the cavity, can be in excess of 500ns for cavities with Q factors above 100 million. As a result of their low material losses, silica microcavities have demonstrated some of the longest photon lifetimes to date1-2. Since a portion of the circulating light extends outside the resonator, these devices can also be used to probe the surroundings. This interaction has enabled numerous experiments in biology, such as single molecule biodetection and antibody-antigen kinetics, as well as discoveries in other fields, such as development of ultra-low-threshold microlasers, characterization of thin films, and cavity quantum electrodynamics studies.3-7
The two primary silica resonant cavity geometries are the microsphere and the microtoroid. Both devices rely on a carbon dioxide laser reflow step to achieve their ultra-high-Q factors (Q&gt;100 million).1-2,8-9 However, there are several notable differences between the two structures. Silica microspheres are free-standing, supported by a single optical fiber, whereas silica microtoroids can be fabricated on a silicon wafer in large arrays using a combination of lithography and etching steps. These differences influence which device is optimal for a given experiment.
Here, we present detailed fabrication protocols for both types of resonant cavities. While the fabrication of microsphere resonant cavities is fairly straightforward, the fabrication of microtoroid resonant cavities requires additional specialized equipment and facilities (cleanroom). Therefore, this additional requirement may also influence which device is selected for a given experiment.
Introduction
An optical resonator efficiently confines light at specific wavelengths, known as the resonant wavelengths of the device. 1-2 The common figure of merit for these optical resonators is the quality factor or Q. This term describes the photon lifetime (&tau;o) within the resonator, which is directly related to the resonator's optical losses. Therefore, an optical resonator with a high Q factor has low optical losses, long photon lifetimes, and very low photon decay rates (1/&tau;o). As a result of the long photon lifetimes, it is possible to build-up extremely large circulating optical field intensities in these devices. This very unique property has allowed these devices to be used as laser sources and integrated biosensors.10
A unique sub-class of resonators is the whispering gallery mode optical microcavity. In these devices, the light is confined in circular orbits at the periphery. Therefore, the field is not completely confined within the device, but evanesces into the environment. Whispering gallery mode optical cavities have demonstrated some of the highest quality factors of any optical resonant cavity to date.9,11 Therefore, these devices are used throughout science and engineering, including in fundamental physics studies and in telecommunications as well as in biodetection experiments. 3-7,12
Optical microcavities can be fabricated from a wide range of materials and in a wide variety of geometries. A few examples include silica and silicon microtoroids, silicon, silicon nitride, and silica microdisks, micropillars, and silica and polymer microrings.13-17 The range in quality factor (Q) varies as dramatically as the geometry. Although both geometry and high Q are important considerations in any field, in many applications, there is far greater leverage in boosting device performance through Q enhancement. Among the numerous options detailed previously, the silica microsphere and the silica microtoroid resonator have achieved some of the highest Q factors to date.1,9 Additionally, as a result of the extremely low optical loss of silica from the visible through the near-IR, both microspheres and microtoroids are able to maintain their Q factors over a wide range of testing wavelengths.18 Finally, because silica is inherently biocompatible, it is routinely used in biodetection experiments.
In addition to high material absorption, there are several other potential loss mechanisms, including surface roughness, radiation loss, and contamination loss.2 Through an optimization of the device size, it is possible to eliminate radiation losses, which arise from poor optical field confinement within the device. Similarly, by storing a device in an appropriately clean environment, contamination of the surface can be minimized. Therefore, in addition to material loss, surface scattering is the primary loss mechanism of concern.2,8
In silica devices, surface scattering is minimized by using a laser reflow technique, which melts the silica through surface tension induced reflow. While spherical optical resonators have been studied for many years, it is only with recent advances in fabrication technologies that researchers been able to fabricate high quality silica optical toroidal microresonators (Q&gt;100 million) on a silicon substrate, thus paving the way for integration with microfluidics.1
The present series of protocols details how to fabricate both silica microsphere and microtoroid resonant cavities. While silica microsphere resonant cavities are well-established, microtoroid resonant cavities were only recently invented.1 As many of the fundamental methods used to fabricate the microsphere are also used in the more complex microtoroid fabrication procedure, by including both in a single protocol it will enable researchers to more easily trouble-shoot their experiments.

We describe the use of a carbon dioxide laser reflow technique to fabricate silica resonant cavities, including free-standing microspheres and on-chip microtoroids. The reflow method removes surface imperfections, allowing long photon lifetimes within both devices. The resulting devices have ultra high quality factors, enabling applications ranging from telecommunications to biodetection.

Fabricação de Sílica Ultra microresonators alto fator de qualidade

Whispering gallery resonant cavities confine light in circular orbits at their periphery.1-2 The photon storage lifetime in the cavity, quantified by the ...

Engenharia

Fabricating Metamaterials Using the Fiber Drawing Method

Metamaterials are man-made composite materials, fabricated by assembling components much smaller than the wavelength at which they operate 1. They owe their electromagnetic properties to the structure of their constituents, instead of the atoms that compose them. For example, sub-wavelength metal wires can be arranged to possess an effective electric permittivity that is either positive or negative at a given frequency, in contrast to the metals themselves 2. This unprecedented control over the behaviour of light can potentially lead to a number of novel devices, such as invisibility cloaks 3, negative refractive index materials 4, and lenses that resolve objects below the diffraction limit 5. However, metamaterials operating at optical, mid-infrared and terahertz frequencies are conventionally made using nano- and micro-fabrication techniques that are expensive and produce samples that are at most a few centimetres in size 6-7. Here we present a fabrication method to produce hundreds of meters of metal wire metamaterials in fiber form, which exhibit a terahertz plasmonic response 8. We combine the stack-and-draw technique used to produce microstructured polymer optical fiber 9 with the Taylor-wire process 10, using indium wires inside polymethylmethacrylate (PMMA) tubes. PMMA is chosen because it is an easy to handle, drawable dielectric with suitable optical properties in the terahertz region; indium because it has a melting temperature of 156.6 &deg;C which is appropriate for codrawing with PMMA. We include an indium wire of 1 mm diameter and 99.99% purity in a PMMA tube with 1 mm inner diameter (ID) and 12 mm outside diameter (OD) which is sealed at one end. The tube is evacuated and drawn down to an outer diameter of 1.2 mm. The resulting fiber is then cut into smaller pieces, and stacked into a larger PMMA tube. This stack is sealed at one end and fed into a furnace while being rapidly drawn, reducing the diameter of the structure by a factor of 10, and increasing the length by a factor of 100. Such fibers possess features on the micro- and nano- scale, are inherently flexible, mass-producible, and can be woven to exhibit electromagnetic properties that are not found in nature. They represent a promising platform for a number of novel devices from terahertz to optical frequencies, such as invisible fibers, woven negative refractive index cloths, and super-resolving lenses.

Metamaterials at terahertz frequencies offer unique opportunities, but are challenging to fabricate in bulk. We adapt the fabrication procedure for microstructured polymer optical fibers to inexpensively fabricate metamaterials potentially on an industrial scale. We produce polymethylmethacrylate fibers containing ~10 &mu;m diameter indium wires separated by ~100 &mu;m, which exhibit a terahertz plasmonic response.

Os metamateriais usando o método de fabricação Desenho de Fibra

Metamaterials are man-made composite materials, fabricated by assembling components much smaller than the wavelength at which they operate 1. They owe ...

Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors

Optical modes of dielectric micro-cavities have received significant attention in recent years for their potential in a broad range of applications. The optical modes are frequently referred to as "whispering gallery modes" (WGM) or "morphology dependent resonances" (MDR) and exhibit high optical quality factors. Some proposed applications of micro-cavity optical resonators are in spectroscopy1, micro-cavity laser technology2, optical communications3-6 as well as sensor technology. The WGM-based sensor applications include those in biology7, trace gas detection8, and impurity detection in liquids9. Mechanical sensors based on microsphere resonators have also been proposed, including those for force10,11, pressure12, acceleration13 and wall shear stress14. In the present, we demonstrate a WGM-based electric field sensor, which builds on our previous studies15,16. A candidate application of this sensor is in the detection of neuronal action potential.
The electric field sensor is based on polymeric multi-layered dielectric microspheres. The external electric field induces surface and body forces on the spheres (electrostriction effect) leading to elastic deformation. This change in the morphology of the spheres, leads to shifts in the WGM. The electric field-induced WGM shifts are interrogated by exciting the optical modes of the spheres by laser light. Light from a distributed feedback (DFB) laser (nominal wavelength of ~ 1.3 &mu;m) is side-coupled into the microspheres using a tapered section of a single mode optical fiber. The base material of the spheres is polydimethylsiloxane (PDMS). Three microsphere geometries are used: (1) PDMS sphere with a 60:1 volumetric ratio of base-to-curing agent mixture, (2) multi layer sphere with 60:1 PDMS core, in order to increase the dielectric constant of the sphere, a middle layer of 60:1 PDMS that is mixed with varying amounts (2% to 10% by volume) of barium titanate and an outer layer of 60:1 PDMS and (3) solid silica sphere coated with a thin layer of uncured PDMS base. In each type of sensor, laser light from the tapered fiber is coupled into the outermost layer that provides high optical quality factor WGM (Q ~ 106). The microspheres are poled for several hours at electric fields of ~ 1 MV/m to increase their sensitivity to electric field.

A high-sensitivity photonic micro sensor was developed for electric field detection. The sensor exploits the optical modes of a dielectric sphere. Changes in the external electric field perturb the sphere morphology leading to shifts in its optical modes. The electric field strength is measured by monitoring these optical shifts.

Desenvolvimento de Whispering Gallery Modo poliméricos Micro-ópticos sensores do campo eléctrico

Optical modes of dielectric micro-cavities have received significant attention in recent years for their potential in a broad range of applications. The ...

Microwave Photonics Systems Based on Whispering-gallery-mode Resonators

Microwave photonics systems rely fundamentally on the interaction between microwave and optical signals. These systems are extremely promising for various areas of technology and applied science, such as aerospace and communication engineering, sensing, metrology, nonlinear photonics, and quantum optics. In this article, we present the principal techniques used in our lab to build microwave photonics systems based on ultra-high Q whispering gallery mode resonators. First detailed in this article is the protocol for resonator polishing, which is based on a grind-and-polish technique close to the ones used to polish optical components such as lenses or telescope mirrors. Then, a white light interferometric profilometer measures surface roughness, which is a key parameter to characterize the quality of the polishing. In order to launch light in the resonator, a tapered silica fiber with diameter in the micrometer range is used. To reach such small diameters, we adopt the "flame-brushing" technique, using simultaneously computer-controlled motors to pull the fiber apart, and a blowtorch to heat the fiber area to be tapered. The resonator and the tapered fiber are later approached to one another to visualize the resonance signal of the whispering gallery modes using a wavelength-scanning laser. By increasing the optical power in the resonator, nonlinear phenomena are triggered until the formation of a Kerr optical frequency comb is observed with a spectrum made of equidistant spectral lines. These Kerr comb spectra have exceptional characteristics that are suitable for several applications in science and technology. We consider the application related to ultra-stable microwave frequency synthesis and demonstrate the generation of a Kerr comb with GHz intermodal frequency.

The customized techniques developed in our lab to build microwave photonics systems based on ultra-high Q whispering gallery mode resonators are presented. The protocols to obtain and characterize these resonators are detailed, and an explanation of some of their applications in microwave photonics is given.

Microwave Systems Fotônica Baseado Ressonadores-gallery de modo Whispering

Microwave photonics systems rely fundamentally on the interaction between microwave and optical signals. These systems are extremely promising for various ...

Fabrication and Characterization of Disordered Polymer Optical Fibers for Transverse Anderson Localization of Light

We develop and characterize a disordered polymer optical fiber that uses transverse Anderson localization as a novel waveguiding mechanism. The developed polymer optical fiber is composed of 80,000 strands of poly (methyl methacrylate) (PMMA) and polystyrene (PS) that are randomly mixed and drawn into a square cross section optical fiber with a side width of 250 &mu;m. Initially, each strand is 200 &mu;m in diameter and 8-inches long. During the mixing process of the original fiber strands, the fibers cross over each other; however, a large draw ratio guarantees that the refractive index profile is invariant along the length of the fiber for several tens of centimeters. The large refractive index difference of 0.1 between the disordered sites results in a small localized beam radius that is comparable to the beam radius of conventional optical fibers. The input light is launched from a standard single mode optical fiber using the butt-coupling method and the near-field output beam from the disordered fiber is imaged using a 40X objective and a CCD camera. The output beam diameter agrees well with the expected results from the numerical simulations. The disordered optical fiber presented in this work is the first device-level implementation of 2D Anderson localization, and can potentially be used for image transport and short-haul optical communication systems.

We develop and characterize a disordered polymer optical fiber that uses transverse Anderson localization as a novel waveguiding mechanism. This microstructured fiber can transport a small localized beam with a radius that is comparable to the beam radius of conventional optical fibers.

Fabricação e caracterização de polímeros desordenados fibras ópticas para Transverse Anderson localização da Luz

We develop and characterize a disordered polymer optical fiber that uses transverse Anderson localization as a novel waveguiding mechanism. The developed ...

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including microresonators. However, because of the increased acoustic radiative losses during direct liquid immersion of optomechanical devices, almost all published optomechanical experiments have been performed in solid phase. This paper discusses a recently introduced hollow microfluidic optomechanical resonator. Detailed methodology is provided to fabricate these ultra-high-Q microfluidic resonators, perform optomechanical testing, and measure radiation pressure-driven breathing mode and SBS-driven whispering gallery mode parametric vibrations. By confining liquids inside the capillary resonator, high mechanical- and optical- quality factors are simultaneously maintained.

Parametric optomechanical excitations have recently been experimentally demonstrated in microfluidic optomechanical resonators by means of optical radiation pressure and stimulated Brillouin scattering. This paper describes the fabrication of these microfluidic resonators along with methodologies for generating and verifying optomechanical oscillations.

Fabricação e Teste de microfluídicos optomechanical osciladores

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including ...

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

Dielectric microspheres can confine light and sound for a length of time through high quality factor whispering gallery modes (WGM). Glass microspheres can be thought as a store of energy with a huge variety of applications: compact laser sources, highly sensitive biochemical sensors and nonlinear phenomena. A protocol for the fabrication of both the microspheres and coupling system is given. The couplers described here are tapered fibers. Efficient generation of nonlinear phenomena related to third order optical non-linear susceptibility &#935;(3) interactions in triply resonant silica microspheres is presented in this paper. The interactions here reported are: Stimulated Raman Scattering (SRS), and four wave mixing processes comprising Stimulated Anti-stokes Raman Scattering (SARS). A proof of the cavity-enhanced phenomenon is given by the lack of correlation among the pump, signal and idler: a resonant mode has to exist in order to obtain the pair of signal and idler. In the case of hyperparametric oscillations (four wave mixing and stimulated anti-stokes Raman scattering), the modes must fulfill the energy and momentum conservation and, last but not least, have a good spatial overlap.

Efficient generation of nonlinear phenomena related to third order optical non-linear susceptibility &#935;(3) interactions in triply resonant silica microspheres is presented in this paper. The interactions here reported are: Stimulated Raman Scattering (SRS), and four wave mixing processes comprising Stimulated Anti-stokes Raman Scattering (SARS).

Estimulada Stokes e Antistokes Raman Scattering em microparticulas Whispering Gallery Modo Resonators

Dielectric microspheres can confine light and sound for a length of time through high quality factor whispering gallery modes (WGM). Glass microspheres ...

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

A &#8220;sheath&#8221; fluid passing through a microfluidic channel at low Reynolds number can be directed around another &#8220;core&#8221; stream and used to dictate the shape as well as the diameter of a core stream.&#160;Grooves in the top and bottom of a microfluidic channel were designed to direct the sheath fluid and shape the core fluid.&#160;By matching the viscosity and hydrophilicity of the sheath and core fluids, the interfacial effects are minimized and complex fluid shapes can be formed.&#160;Controlling the relative flow rates of the sheath and core fluids determines the cross-sectional area of the core fluid.&#160;Fibers have been produced with sizes ranging from 300 nm to ~1 mm, and fiber cross-sections can be round, flat, square, or complex as in the case with double anchor fibers. Polymerization of the core fluid downstream from the shaping region solidifies the fibers.&#160;Photoinitiated click chemistries are well suited for rapid polymerization of the core fluid by irradiation with ultraviolet light.&#160;Fibers with a wide variety of shapes have been produced from a list of polymers including liquid crystals, poly(methylmethacrylate), thiol-ene and thiol-yne resins, polyethylene glycol, and hydrogel derivatives. Minimal shear during the shaping process and mild polymerization conditions also makes the fabrication process well suited for encapsulation of cells and other biological components.

Two adjacent fluids passing through a grooved microfluidic channel can be directed to form a sheath around a prepolymer core; thereby&#160;determining both shape and cross-section. Photoinitiated polymerization, such as thiol click chemistry, is well suited for rapidly solidifying the core fluid into a microfiber with predetermined size and shape.

Fabricação microfluida de fibras poliméricas e biohibridas com tamanho e forma predesigned

A &#8220;sheath&#8221; fluid passing through a microfluidic channel at low Reynolds number can be directed around another &#8220;core&#8221; stream and ...

Bioengenharia

Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation

We present a protocol for fabricating 1-D Photonic Crystal (PhC) cavities on subwavelength-diameter tapered optical fibers, optical nanofibers, using femtosecond laser-induced ablation. We show that thousands of periodic nano-craters are fabricated on an optical nanofiber by irradiating with just a single femtosecond laser pulse. For a typical sample, periodic nano-craters with a period of 350 nm and with diameter gradually varying from 50 - 250 nm over a length of 1 mm are fabricated on a nanofiber with diameter around 450 - 550 nm. A key aspect of such a nanofabrication is that the nanofiber itself acts as a cylindrical lens and focuses the femtosecond laser beam on its shadow surface. Moreover, the single-shot fabrication makes it immune to mechanical instabilities and other fabrication imperfections. Such periodic nano-craters on nanofiber, act as a 1-D PhC and enable strong and broadband reflection while maintaining the high transmission out of the stopband. We also present a method to control the profile of the nano-crater array to fabricate apodized and defect-induced PhC cavities on the nanofiber. The strong confinement of the field, both transverse and longitudinal, in the nanofiber-based PhC cavities and the efficient integration to the fiber networks, may open new possibilities for nanophotonic applications and quantum information science.

We present a protocol for fabricating 1-D photonic crystal cavities on subwavelength diameter silica fibers (optical nanofibers) using femtosecond laser-induced ablation.

Fabricação de 1-D cristal fotônico cavidade em um nanofibras com ablação induzida por laser femtosegundo

We present a protocol for fabricating 1-D Photonic Crystal (PhC) cavities on subwavelength-diameter tapered optical fibers, optical nanofibers, using ...

Fabrication of High-Quality Whispering Gallery Mode Microbubble Resonators

We demonstrate a robust and standardized method for the fabrication of high-quality factor (Q-factor) Whispering Gallery Mode (WGM) Microbubble resonators (MBRs) with a precision glass processing machine (PGP). Microbubble resonators are a unique class of WGM devices with integrated fluidic channels, making them ideal for diverse sensing applications. Herein, we show a standardized protocol to fabricate high-Q microbubble resonators through the optimization of key performance metrics, such as Q-factor and wall thickness. We also show methods to improve the sensitivity of the platform to refractive index changes and other sensing targets through Hydrofluoric acid (HF) wet etching. Lastly, a brief analysis of the resistance of microbubbles to fluid flow is discussed, showing that smaller-diameter microbubbles exhibit greater resistance to flow for analyte delivery - a factor that should be considered for analyte delivery. The implementation of this refined fabrication protocol not only increases the success rate of device production but also reduces fabrication time. Moreover, the protocol can be expanded to other techniques used to produce MBRs, such as CO2 laser-based methods.

Here, we demonstrate a robust and standardized protocol for fabricating high-quality factor (Q-factor) Whispering Gallery Mode (WGM) Microbubble resonators (MBRs) with a precision glass processing machine (PGP).

Fabricação de ressonadores de microbolhas em modo de galeria sussurrante de alta qualidade

We demonstrate a robust and standardized method for the fabrication of high-quality factor (Q-factor) Whispering Gallery Mode (WGM) Microbubble resonators ...