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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

2.4K Views

06:49 min

May 24th, 2022

DOI :

10.3791/63953-v

May 24th, 2022


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Lentiviral Delivery

Rozdziały w tym wideo

0:04

Introduction

0:28

Transfection of HEK293T Cells Using Either Polymer Polyethylenimine (PEI) or Lipofectamine 3000 Reagent

2:48

Lentiviral Vectors (LVS) Collection and Ultracentrifugation

5:07

Results: Lentiviral Mediated Delivery of shRNAs in hESCs and NPCs Using Cationic Polymer PEI

6:19

Conclusion

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