Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
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12.9K Views
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10:52 min
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June 5th, 2015
DOI :
June 5th, 2015
•Rozdziały w tym wideo
0:05
Title
1:44
Obtaining Peripheral Blood Mononuclear Cells (PBMNCs) from Buffy Coats
2:46
Culturing PBMNCs Obtained from Buffy Coats
4:36
Transfecting PBMNCs
6:19
Culturing Transfected PBMNCs on Mouse Embryonic Fibroblasts (MEFs)
7:32
Generating Induced Pluripotent Stem Cells (iPSC) Clones
8:22
Results: Cellular Characteristic from PBMNC to iPSC
10:21
Conclusion
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