JoVE Logo

Zaloguj się

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

4.5K Views

09:50 min

December 25th, 2021

DOI :

10.3791/63038-v

December 25th, 2021


Przeglądaj więcej filmów

Digital Droplet PCR

Rozdziały w tym wideo

0:01

Introduction

0:57

Small Scale AAV Production

2:52

Intravitreal Injection of AAV

4:17

Fluorescence and Fundus Imaging

4:48

Retina Isolation

5:16

Tissue Genomic DNA Isolation

5:57

Digital Droplet PCR Analysis of Mouse Retina Samples for Quantification of Viral Genomes

7:52

Results

9:07

Conclusion

Powiązane Filmy

article

10:14

Using Adeno-associated Virus as a Tool to Study Retinal Barriers in Disease

15.0K Views

article

09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

2.6K Views

article

09:03

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

4.1K Views

article

13:13

Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations

2.8K Views

article

04:43

Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction

1.7K Views

article

14:49

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

13.6K Views

article

Longitudinal In Vivo Imaging of Retinal Cells with Tailored Adeno-Associated Virus Serotypes via Confocal Scanning Laser Ophthalmoscopy

394 Views

article

Measurement of Mitochondrial Membrane Potential In Vivo using a Genetically Encoded Voltage Indicator

236 Views

article

05:03

Consistent Delivery of Adeno-Associated Virus via Lateral Tail-Vein Injection in Adult Mice

960 Views

article

05:53

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

4.4K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone