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Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

8.9K Views

11:33 min

March 22nd, 2019

DOI :

10.3791/59246-v

March 22nd, 2019


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Calcium Signals

Rozdziały w tym wideo

0:04

Title

1:10

Preparation of Poly(ethyleneimine) Coated Coverslips

2:42

Preparation of Rat Cortical Neuron-astrocyte Mixed Culture and Frozen Cells, and the Revival of Frozen Cultures

5:45

Transfection and Adeno-associated Virus Infection of Hippocampal or Cortical Neurons

7:29

Recording Spontaneous Activities of Astrocytes Expressing Lck-RCaMP2 and OER-GCaMP6f

8:37

Results: Dissection of Local Ca2+ Signals in Cultured Cells

10:38

Conclusion

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