A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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10:42 min
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December 12th, 2017
DOI :
December 12th, 2017
•Rozdziały w tym wideo
0:05
Title
0:52
Transfecting of HEK-293T Cells Using Calcium Phosphate
2:19
Harvesting Virus
2:50
Concentration of Viral Particles by Ultracentrifugation
5:11
Esimating of Viral Titers Using p24-enzyme-linked Immunosorbent Assay
7:31
Counting GFP-positive Cells
8:25
Results: Production and Validation of the Knockout-efficiency of Integrase-deficient Lentiviral-CRSPER/Cas9 Vector
9:57
Conclusion
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