細胞分裂に CRISPR/Cas9 を介した遺伝子ノックアウトのインテグラーゼ欠損レンチウイルスベクターの生産のためのプロトコル

0:52

Transfecting of HEK-293T Cells Using Calcium Phosphate

2:19

Harvesting Virus

2:50

Concentration of Viral Particles by Ultracentrifugation

5:11

Esimating of Viral Titers Using p24-enzyme-linked Immunosorbent Assay

7:31

Counting GFP-positive Cells

8:25

Results: Production and Validation of the Knockout-efficiency of Integrase-deficient Lentiviral-CRSPER/Cas9 Vector

9:57

Conclusion