A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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11:25 min
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March 18th, 2017
DOI :
March 18th, 2017
•Rozdziały w tym wideo
0:05
Title
0:54
Lyse Bacterial Pellets Expressing Human Recombinant G4 Resolvase1 (rG4R1)
3:06
Prepare G4-magnetic Beads
4:07
Bind Histidine-tagged rG4R1 to Cobalt Beads
5:47
Elute rG4R1 from Cobalt Beads
7:07
Bind rG4R1 to G4-bound SPB and Elute in an ATP-dependent Manner
8:55
Results: Analysis and Quantification of Catalytically Active, Purified rG4R1
10:06
Conclusion
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