Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
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11:27 min
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March 16th, 2017
DOI :
March 16th, 2017
•Rozdziały w tym wideo
0:05
Title
1:53
3D Caco-2 Cell Culture on a Gelatinous Protein Solution
3:23
Immunofluorescence Staining of Epithelial Transporters in 3D Caco-2 Cells
5:12
Measurement of 5-HT Uptake in Mouse Ileum
6:29
Equilibration and Tissue Treatment
7:41
Quantification of the Mucosal to serosal Flux (Jm-s) and 3[H]-5-HT Accumulation
8:42
Results: The Expression and Function of SERT Protein Measured in Caco-2 3D Cell Cultures and Ileal Mucosa Ex Vivo
9:57
Conclusion
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