A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
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10:13 min
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July 3rd, 2013
DOI :
July 3rd, 2013
•Rozdziały w tym wideo
0:05
Title
1:54
Preparation of Cells for siRNA and Reporter Transfection
5:42
Adding Transfection Mixtures to Cells
4:10
Preparing siRNA/DNA Transfection Mixtures
6:21
Determine Luciferase Activities from Cell Samples
8:04
Results: High-throughput Interrogation of Gene Function in Wnt/β-catenin, p53, and Kras Singaling
9:53
Conclusion
3:22
Preparing Reporter Construct DNA
3:19
siRNA and Reporter Transfection of Cells
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