Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Please note that all translations are automatically generated. Click here for the English version.
4.7K Views
•
12:41 min
•
December 23rd, 2022
DOI :
December 23rd, 2022
•Maxim Le Compte1, Edgar Cardenas De La Hoz2, Sofía Peeters1, Evelien Smits1, Filip Lardon1, Geert Roeyen1,3, Steve Vanlanduit2, Hans Prenen1,4, Marc Peeters1,4, Abraham Lin1,5, Christophe Deben1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp
Przeglądaj więcej filmów
Rozdziały w tym wideo
0:05
Introduction
0:53
Day 0: Preparation of the 2- or 3-Day-Old Organoids
2:55
Days 2 - 3: Harvesting the 2- or 3-Day-Old Organoids
4:16
Seeding the 2- or 3-Day-Old Organoids Using a Pipetting Robot
5:27
Day 4: Drug Treatment and Reagent Dispensing with Digital Drug Dispenser
7:49
Image Acquisition with the Live-Cell Imager and Analysis of Data
10:12
Results: The Single-Organoid Dose-Response Analysis
12:03
Conclusion
Powiązane Filmy
11:20
Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
10.8K Views
10:04
Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
11.9K Views
08:27
Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices
11.3K Views
Medium-Throughput Drug- and Radiotherapy Screening Assay using Patient-Derived Organoids
3.7K Views
06:25
High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids
5.2K Views
08:52
Profiling Sensitivity to Targeted Therapies in EGFR-Mutant NSCLC Patient-Derived Organoids
3.9K Views
10:13
Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
2.1K Views
09:13
Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer
1.0K Views
10:40
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
15.9K Views
07:42
A High-Throughput Platform for Culture and 3D Imaging of Organoids
2.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone