Substrate Generation for Endonucleases of CRISPR/Cas Systems
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11:53 min
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September 8th, 2012
DOI :
September 8th, 2012
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Rozdziały w tym wideo
0:05
Title
1:37
CRISPR/Cas System Overview
2:29
Generation of Long Pre-crRNA Substrates via PCR
4:14
Generation of Intermediate Pre-cdRNA Substrates via Annealing of DNA Oligonucleotides
6:10
Generation of Short Cas RNA Substrates via Custom RNA Oligonucleotide Synthesis
6:41
In vitro T7 RNA Polymerase Transcription
8:04
Cas6 Endonuclease Assay
9:08
Results: Representative Cas6 Endonuclease Assay
11:10
Conclusion
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