Name: tape: a biodegradable hemostatic glue inspired by a ubiquitous compound in plants for surgical application
Uploaded: 2016-06-08T13:00:00.000+00:00
Duration: 8 min 40 s
Description: Watch this Scientific Journal Video about TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application at JoVE.com

This study was supported by National Research Foundation of South Korea: Mid-career scientist grant (2014002855), and Ministry of Industry, Trade, and Natural Resources: World Premier Material Development Program. This work is also supported by in part by Center for Nature-inspired Technology (CNiT) in KAIST Institute for NanoCentury (KINC).

모든 동물 관리 및 실험은 KAIST (한국 과학 기술원)에서 제공하는 윤리적 프로토콜 IRB (임상 시험 심사위원회)에 따라 수행된다. 1. TAPE 형성 <ol><li> 트랜스 용액의 제조 마그네틱 스터에 4 mL의 크기의 유리 병을 놓고, 교반 막대 증류수 1 ㎖를 추가한다. 바이알에 탄닌산 1 g을 넣고, 이상에서 1 시간 동안 200 rpm에서 온화하게 교반하여 물에 녹인다. TA에 완전히 용해 될 때, 혼합물은 갈색 투명해진다. </li><li> 백색 슬러리를 만들기 위해 몇 초 동안 볼 텍싱하여 이들을 혼합하여 증류수 1 ㎖ 다음에 PEG 분말 1g (4 아암 10 kDa의, 리니어, 4.6 kDa의)을 첨가하여 PEG 용액을 제조 하였다. 10 분 동안 60 ° C의 배양기에서 슬러리를 유지한다. 흰색 하나는 완전히 명확해질 때까지. 참고 : 10 kDa의 분자량을 갖는 PEG의 융점이 약 55 인- 60 ℃, 및 4 kDa의 하나는 53 - 58 °의 C. 1g에 물까지의 PEG의 고농도 / ㎖의 투명한 용액으로 얻어 질 수 있도록 용융 된 PEG는 수혼 화성된다. 맑은 PEG 용액을 고온에서 형성되면, 용액을 계속 냉각 한 후, 실온에서 안정하다. </li><li> PEG의 329 μl를 추가 (4 아암 10 kDa의) 용액 4.6 kDa의 갖는 선형 PEG의 경우 1.1 단계에서 얻어진 TA 용액 (1.2 671 μL를 위해 PEG 용액 311 μL를 추가 단계에서 제조 한 마이크로 원심 분리기 튜브에서 TA 용액) 689 μL. 조심스럽게 균일를 혼합 좁은 주걱 두 점성과 꿀 같은 솔루션을 혼합. 주의 : 두 솔루션은 매우 점성, 그래서 과학자가 느리지 만 충분히 끌어와 마이크로 피펫으로 솔루션을 전송해야합니다. </li><li> 고정 된 앵글 로터를 구비 한 원심 분리기에서 3 분 동안 12,300 × g으로 1.3로 공정에서 제조 한 혼합물을 스핀. </li><li> 조심스럽게 렘마이크로 피펫을 사용하여 가능한 한 상등액만큼 비켜하고 침착 한 생성물을 수집 :이 완전히 형성 TAPE이다. . TAPE 형성 한 후, 냉장고에서 보관 - 몇 주까지 NOTE (4 8 °의 C) : 테이프 감마선 또는 외과 적 용도로 사용하기 전에, 전자 빔 처리에 의해 살균 될 수있다. </li></ol> 테이프의 접착 강도의 측정 (2) <ol><li> 피부 조직의 모든 지방을 제거한 후, 생검 펀치로 절단하여 6mm 직경의 돼지의 피부 조직의 두 개를 준비한다. 주 : 돼지 피부 조직이 건강한 돼지 측면 피부에서 얻어진 것으로, 한국 대전에 위치한 지역 고기 시장에서 구입했다. </li><li> 각 조직의 외 측면에 상업 시아 노 아크릴 레이트 접착제를 적용하고, 금속 막대에 조직을 연결합니다. 참고 : 금속로드가 보조 핸들이 그래서를 티슈 사용직접 기계에 의해 사로 잡혔다하지 않았습니다. 따라서, 인장 기의 구성을 다음과 같은 다른 물질로 대체 될 수있다. </li><li> 티슈의 한쪽 - TAPE 방울을 적용 (6 mg의 테이프의 드롭은 약 3 임). 이어서,도 2a에 도시 한 바와 같이 이들이 부착되도록 균일 그 내측에있는 두 개의 다른 조직 사이의 조직을 사용하여 테이프를 확산. </li><li> 그 후, 수동으로 균일하게 혼합하고, 각 조직 테이프 사이의 계면을 극대화 수회 부착 조직의 양쪽 분리. </li><li> UTM을로,로드의주의 그립 각면. 접착 강도는 테이프 부착 개의 조직을 분리하는 데 필요한 힘에 의해 결정된다. 첫째, 1 분 20 N의 힘을 적용합니다. 이어서, 기계로, 1mm / min의 속도로 반대 방향으로 각로드를 당긴다. 때까지 조직을 완전히 분리된다. 주 : 데이터는 힘 - 거리로 주어질 것이다 (FD) 곡선 움직임 검출각로드. </li><li> 즉, 시편 표면적에 의해 단계 250에서 달성 된 FD 곡선에 도시 된 최대 힘 (KN)을 분할하여 테이프의 접착 강도를 계산 3.14 × (0.003 m) 2. </li><li> 물의 존재 하에서 접착 강도를 모니터링하기 위해, 두 조직 사이의 분리 영역 상에 20 μL의 물을 추가하고, 즉시 첨부. 기계로 다시 분리 테스트를 수행합니다. </li></ol> 3. 체외 저하 테스트 <ol><li> 마이크로 원심 분리 튜브의 캡 (d = 8 mm)를 잘라내, 및 W의 C로 정의 캡을 단다. </li><li> TAPE 150 mg을 가진 캡을 입력하고 0 W 총 초기 무게로 설정을 다시 모두 함께 무게. 주의 : 모자 TAPE를 너무 많이 넣지 마십시오. 이 단계 3.4에서 배양 동안 교반 처리에 의해 생성 된 PBS 완충액의 흐름에 대한 물리적 장벽을 될 수있는 테이프의 높이가 상기 캡의 상단보다 낮아야한다. </li><li&gt; 세포 배양 용 플라스크에 테이프를 포함하는 캡을 넣어 (75cm 2) 및 PBS 완충액 50 mL로 (1X, pH 7.4의) 세포 배양 플라스크에 뚜껑 테이프가 완전히 같이, PBS 완충액에 침지되도록 추가 도 3a에 도시 한 (N = 5). </li><li> 부드럽게 교반 (50 RPM)와 생리 학적 조건과 유사한 37 ℃에서 오비탈 진탕 배양기 단계 3.3에서 제조 된 세포 배양 플라스크를 인큐베이션. 주의 : 50 rpm으로 교반 상태를 유지합니다. 높은 RPM은 TAPE의 붕괴가 발생할 수 있습니다. </li><li> 각 시점에서, 세포 배양 플라스크에서 테이프로 뚜껑을 한 후, 질소 가스를 불어 건조한다. 남은 테이프를 포함하는 캡을 단다. W t의 각 시점에서의 무게를 설정한다. 다시 신선한 PBS를 교체하고, 각 시점에서 W (T)를 측정 한 후이를 다시 흔들. </li><li> 상대적인 잔류 중량 (%)은 다음 식을 계산한다. 상대 남은 중량 (%) = (W의 t </suB&gt; - W의 C) / (W 0 - W C) × 100 % </li></ol> TAPE 4. 지혈 능력 주 : 모든 동물 실험은 보건 복지부 한국 정부가 제공하는 가이드 라인과 윤리 프로토콜에 따라 수행되어야한다. <ol><li> REF (24)에 기재된 바와 같이 생체 내 지혈 능력을 평가하기 위해, 마우스를 출혈 간 모델을 검토. </li><li> (그룹 당 n = 5) tiletamine-zolazepam (33.333 ㎎ / ㎏)과 자일 라진 (7.773 ㎎ / ㎏)의 복강 내 주사와 - 십오 마우스 (남성 35g, 정상 ICR 마우스 6 주, 30) 마취. 적절한 마취를 확인하려면, 동물의 발을 부드럽게 꼬집어 어떤 운동이 동물이 수술을하기에 충분히 마취 있음을 나타냅니다 등, 발을 인출하는 등의 움직임을 관찰한다. </li><li> 동물의 눈의 건조를 방지하려면 아래에 충분히 동안 눈에 수의사 연고를 적용하려면esthesia. 중간 선 복부 절개를 통해 간을 노출하고 출혈을 유도하기 위해 18 G 바늘로 간을 찌르. </li><li> 멸균 거즈로 흐르는 혈액을 제거하고 출혈 사이트에 즉시 TAPE 또는 피브린 접착제 (양성 대조군) 100 ㎕를 넣어. 참고 : 더 이상 봉합으로 인해 상처 조직에 대한 고도의 혈액에 강한 접착 특성에 TAPE를 적용한 후 필요하지 않습니다. 음성 대조군의 경우, 치료는 출혈의 사이트에서 발생하지 않는다. </li><li> 각각의 경우, 파손 부위로부터 혈액을 수집 간 아래 공지 질량 여과지 넣어. 새로운 일 4 회 매 30 초 (즉., 2 분)으로 용지를 교체합니다. </li><li> 매 30 초마다 수집 된 여과지에 흡수 된 혈액의 질량을 측정한다. 동물 실험 후, CO 2 질식의 안락사를 통해 쥐를 희생. </li></ol>

<ol>
	<li>Leggat, P. A., Smith, D. R., Kedjarune, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+applications+of+cyanoacrylate+adhesives:+a+review+of+toxicity.">Surgical applications of cyanoacrylate adhesives: a review of toxicity.</a> ANZ J Surg. 77 (4), 209-213 (2007).</li><li>MacGillivray, T. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrin+Sealants+and+Glues.">Fibrin Sealants and Glues.</a> J Cardiac Surg. 18 (6), 480-485 (2003).</li><li>Radosevich, M., Goubran, H. A., Burnouf, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrin+sealant:+scientific+rationale,+production+methods,+properties+and+current+clinical+use.">Fibrin sealant: scientific rationale, production methods, properties and current clinical use.</a> Vox. Sang. 72 (3), 133-143 (1997).</li><li>Nomori, H., Horio, H., Suemasu, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+efficacy+and+side+effects+of+gelatin-resorcinol+formaldehyde-glutaraldehyde+(GRFG)+glue+for+preventing+and+sealing+pulmonary.">The efficacy and side effects of gelatin-resorcinol formaldehyde-glutaraldehyde (GRFG) glue for preventing and sealing pulmonary.</a> Surg. Today. 30 (3), 244-248 (2000).</li><li>Duarte, A. P., Coelho, J. F., Bordado, J. C., Cidade, M. T., Gil, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+adhesives:+Systematic+review+of+the+main+types+and+development+forecast.">Surgical adhesives: Systematic review of the main types and development forecast.</a> Prog. Polym. Sci. 37 (8), 1031-1050 (2012).</li><li>Bhatia, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Traumatic+injuries.">Traumatic injuries.</a> Biomaterials for clinical applications. , 213-258 (2010).</li><li>Bouten, P. J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+chemistry+of+tissue+adhesive+materials.">The chemistry of tissue adhesive materials.</a> Prog.Polym. Sci. 39 (7), 1375-1405 (2014).</li><li>Annabi, N., Tamayol, A., Shin, S. R., Ghaemmaghami, A. M., Peppas, N. A., Khademhosseini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+materials:+Current+challenges+and+nano-enabled+solutions.">Surgical materials: Current challenges and nano-enabled solutions.</a> Nano Today. 9 (5), 574-589 (2014).</li><li>Strausberg, R. L., Link, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protein-based+medical+adhesives.">Protein-based medical adhesives.</a> Trends.Biotechnol. 8 (2), 53-57 (1990).</li><li>Bilic, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injectable+candidate+sealants+for+fetal+membrane+repair:+bonding+and+toxicity+in+vitro.">Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.</a> Am J Obstet Gynecol. 202 (1), 1-9 (2010).</li><li>Mehdizadeh, M., Weng, H., Gyawali, D., Tang, L., Yang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injectable+citrate-based+mussel-inspired+tissue+bioadhesives+with+high+wet+strength+for+sutureless+wound+closure.">Injectable citrate-based mussel-inspired tissue bioadhesives with high wet strength for sutureless wound closure.</a> Biomaterials. 33 (32), 7972-7980 (2012).</li><li>Ryu, J. H., Lee, Y., Kong, W. H., Kim, T. G., Park, T. G., Lee, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catechol-functionalized+chitosan/pluronic+hydrogels+for+tissue+adhesives+and+hemostatic+materials.">Catechol-functionalized chitosan/pluronic hydrogels for tissue adhesives and hemostatic materials.</a> Biomacromolecules. 12 (7), 2653-2660 (2011).</li><li>Mahdavi, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+biodegradable+and+biocompatible+gecko-inspired+tissue+adhesive.">A biodegradable and biocompatible gecko-inspired tissue adhesive.</a> Proc Natl Acad Sci USA. 105 (7), 2307-2310 (2008).</li><li>Lee, H., Lee, B. P., Messersmith, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+reversible+wet/dry+adhesive+inspired+by+mussels+and+geckos.">A reversible wet/dry adhesive inspired by mussels and geckos.</a> Nature. 488, 338-341 (2007).</li><li>Yang, S. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+bio-inspired+swellable+microneedle+adhesive+for+mechanical+interlocking+with+tissue.">A bio-inspired swellable microneedle adhesive for mechanical interlocking with tissue.</a> Nature Commun. 4, 1702-1710 (2013).</li><li>Spotnitz, W. D., Burks, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hemostats,+sealants,+and+adhesives:+components+of+the+surgical+toolbox.">Hemostats, sealants, and adhesives: components of the surgical toolbox.</a> Transfusion (Paris). 48 (7), 1502-1516 (2008).</li><li>Erel, I., Schlaad, H., Demirel, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+structural+isomerism+and+polymer+end+group+on+the+pH-stability+of+hydrogen-bonded+multilayers.">Effect of structural isomerism and polymer end group on the pH-stability of hydrogen-bonded multilayers.</a> J Colloid Interface Sci. 361 (2), 477-482 (2011).</li><li>Shutava, T. G., Prouty, M. D., Agabekov, V. E., Lvov, Y. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidant+Properties+of+Layer-by-Layer+films+on+the+Basis+of+Tannic+Acid.">Antioxidant Properties of Layer-by-Layer films on the Basis of Tannic Acid.</a> Chem Lett. 35 (10), 1144-1145 (2006).</li><li>Schmidt, D. J., Hammond, P. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemically+erasable+hydrogen-bonded+thin+films.">Electrochemically erasable hydrogen-bonded thin films.</a> Chem Commun. 46 (39), 7358-7360 (2010).</li><li>Shutava, T., Prouty, M., Kommireddy, D., Lvov, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=pH+Responsive+Decomposable+Layer-by-Layer+Nanofilms+and+Capsules+on+the+Basis+of+Tannic+Acid.">pH Responsive Decomposable Layer-by-Layer Nanofilms and Capsules on the Basis of Tannic Acid.</a> Macromolecules. 38 (7), 2850-2858 (2005).</li><li>Erel, I., Zhu, Z., Zhuk, A., Sukhishvili, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrogen-bonded+layer-by-layer+films+of+block+copolymer+micelles+with+pH-responsive+cores.">Hydrogen-bonded layer-by-layer films of block copolymer micelles with pH-responsive cores.</a> J Colloid Interface Sci. 355 (1), 61-69 (2011).</li><li>Kim, B. -. S., Lee, H. -. I., Min, Y., Poon, Z., Hammond, P. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrogen-bonded+multilayer+of+pH-responsive+polymeric+micelles+with+tannic+acid+for+surface+drug+delivery.">Hydrogen-bonded multilayer of pH-responsive polymeric micelles with tannic acid for surface drug delivery.</a> Chem Commun. 45 (28), 4194-4196 (2009).</li><li>Murakami, Y., Yokoyama, M., Nishida, H., Tomizawa, Y., Kurosawa, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+hemostasis+model+for+the+quantitative+evaluation+of+hydrogel-based+local+hemostatic+biomaterials+on+tissue+surface.">A simple hemostasis model for the quantitative evaluation of hydrogel-based local hemostatic biomaterials on tissue surface.</a> Colloids Surf B Biointerfaces. 65 (2), 186-189 (2008).</li><li>Kim, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TAPE:+A+Medical+Adhesive+Inspired+by+a+Ubiquitous+Compound+in+Plants.">TAPE: A Medical Adhesive Inspired by a Ubiquitous Compound in Plants.</a> Adv Funct Mater. 25 (16), 2402-2410 (2015).</li><li>Suzuki, S., Ikada, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesion+of+cells+and+tissues+to+bioabsorbable+polymeric+materials:+scaffolds,+surgical+tissue+adhesives+and+anti-adhesive+materials.">Adhesion of cells and tissues to bioabsorbable polymeric materials: scaffolds, surgical tissue adhesives and anti-adhesive materials.</a> J Adhes. Sci. Technol. 24 (13), 2059-2077 (2010).</li><li>Lomova, M. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multilayer+Capsules+of+Bovine+Serum+Albumin+and+Tannic+Acid+for+Controlled+Release+by+Enzymatic+Degradation.">Multilayer Capsules of Bovine Serum Albumin and Tannic Acid for Controlled Release by Enzymatic Degradation.</a> ACS Appl Mater Interfaces. 7 (22), 11732-11740 (2015).</li><li>Shin, M., Ryu, J. H., Park, J. P., Kim, K., Yang, J. W., Lee, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DNA/Tannic+Acid+Hybrid+Gel+Exhibiting+Biodegradability,+Extensibility,+Tissue+Adhesiveness,+and+Hemostatic+Ability.">DNA/Tannic Acid Hybrid Gel Exhibiting Biodegradability, Extensibility, Tissue Adhesiveness, and Hemostatic Ability.</a> Adv Funct Mater. 25 (8), 1270-1278 (2015).</li><li>Kozlovskaya, V., Kharlampieva, E., Drachuk, I., Chenga, D., Tsukruk, V. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Responsive+microcapsule+reactors+based+on+hydrogen-bonded+tannic+acid+layer-by-layer+assemblies.">Responsive microcapsule reactors based on hydrogen-bonded tannic acid layer-by-layer assemblies.</a> Soft Matter. 6 (15), 3596-3608 (2010).</li><li>Oh, D. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+rapid,+efficient,+and+facile+solution+for+dental+hypersensitivity:+The+tannin-iron+complex.">A rapid, efficient, and facile solution for dental hypersensitivity: The tannin-iron complex.</a> Sci Rep. 5, 10884 (2015).</li></ol>

The authors have nothing to disclose.

우리는 식물 유래 폴리 페놀 화합물, TA의 방수 분자 상호 작용에 의해 영감을 지혈 접착제라는 TAPE의 완전히 새로운 클래스를 개발했다. TA 크게 인해 산화 방지제, 항균 항 돌연변이 및 항암 특성으로 주목을 받고 대표 가수 분해성 탄닌이다. 그것은 더 화학 합성 과정없이 다음, 원심 분리하여 두 수용액의 단지 한 단계 혼합 같이 테이프를 제조하는 과정은 매우 간단하고 확...

과거 10 년의 노력으로 인해 수술 적 치료시 사용과 낮은 조직 침입에 자신의 편의에 생분해 성 / 흡수성 접착제와 상처를 닫 현재 수술 봉합 및 스테이플을 교체되었습니다. 시판 조직 접착제는 네 가지 유형으로 분류된다 : (1) 시아 노 아크릴 레이트 유도체 (1) (2) 2,3- 트롬빈에 의해 중합체를 피브린, 피브리노겐으로부터 효소 적 전환에 의해 형성된 피브린 접착제를, (3) 등의 화학적 또는 물리적으로 같은 단백질 계 재료 가교 알부민 및 / 또는 젤라틴 4.5, (4) 합성 폴리머 계 것 6. 그들은 많은 임상 용도에 사용되어 왔지만, 모두 접착제는 자신 고유의 단점들이 널리 사용 장애물이 될 수있는 단점이있다. 시아 노 아크릴 레이트 계 접착제는 조직에 대한 높은 접착 강도를 보여 주지만, 이들 독성 부산물 등의 분해 동안 형성 및 시아 노 포름 종종 부호 원인염증 반응 (7)의 ificant도. 젤라틴 기반의 접착제 8 소, 닭, 돼지, 물고기를 포함하여 피브린 접착제과 동물에 대한 인간의 혈장 : 피브린 접착제 및 알부민 또는 젤라틴 기반 물질은 동물의 소스에서 바이러스와 같은 감염 구성 요소의 전송에 대한 안전 문제가 있습니다. 약간의 합성수지 계 접착제 연방 의약품 안전청 (FDA)에 의해 승인되었지만, 합성 중합체로 만들어진 대부분의 접착제는 제조 공정 단계를 최소화하고 생체 적합성 (9)을 달성하는데 어려움을 계속한다. 가장 중요한 것은, 모든 접착제는 물티슈 10 가난한 기계 및 접착 강도 고통. 최근 해양 홍합 11-13, 도마뱀 붙이 (14), 홍합 15 도마뱀, 그리고 endoparasitic 웜 (16)에 의해 영감을 생체 모방 조직 접착제는 그들의 조정 가능한 기계적으로 현재 의료 접착제에 관한 유망한 대안을 신흥되었습니다생체 적합성과 접착 특성. 그들은 상용 제품 (17)되기 전에 그러나, 오늘날까지 여전히 해결해야 할 문제가 있습니다. 여기서는, 식물 유래 접착 분자, 탄닌산 (TA), 및 생체 불활성 중합체 폴리 (에틸렌 글리콜) (PEG)의 분자간 수소 결합에 의해 제조 TAPE라는 의료용 접착제 완전히 새로운 유형을보고 그 이름에서 알 수 있듯이. TA는 보편적으로 식물의 2 차 대사 중에 발견 대표 가수 분해성 탄닌이다. 또한, 항 돌연변이 및 항암 성질 인하여 항산화에 많은 주목을 받고 있으며, 폴리 (N의 이소 프로필 아크릴 아미드) (PNIPAM) 및 폴리 (N만큼 중합체와 초분자 상호 작용에 참여하는 것으로 나타났다 - 비닐 피 롤리 돈) (PVPON), 계층별로 레이어 (LBL) 영화 18 ~ 20 약물 방출 마이크로 캡슐 21 ~ 23을 형성한다. 본 연구에서는 TA 효율적인 역할을 할 수 있음을 발견방수 접착제 관능기 테이프, 의료 접착제를 형성한다. TA 간단한 혼합하여, 비 오염 중합체 PEG를 갖는 초분자 접착제하게 2.5 배 상업적 피브린 접착제에 비하여 증가 된 접착 강도를,이 밀착성에도 물의 존재 하에서, 착탈의 최대 20주기 동안 유지시켰다 . 그 지혈 능력은 생체 내에서 간 출혈 모델을 테스트하고 몇 초 내에 출혈을 멈추게하는 좋은 지혈 능력을 보여 주었다. TAPE 바이오 영감 접근 방식과 현재 문제의 단점을 해결에 새로운 통찰력을 공개 할 수있는 최초의 식물 유래 접착제로서 관련 분야에서의 중요한 의미를 갖습니다. 우리는 또한 간단한 제조 방법에있어서, 확장 성, 가변 생분해 속도 등의 뮤코 접착제, 약물 방출 패치, 상처 치료 드레싱 등과 같은 의약 다양한 용도 TAPE의 광범위한 사용을 기대할뿐만 아니라 높은 습기에 강한 ADHES이온 특성.

<table><tbody><tr><td>Tannic acid</td><td>Sigma-aldrich</td><td>403040</td><td></td></tr><tr><td>Poly(ethylene oxide), 4-arm, hydroxy terminated</td><td>Aldrich</td><td>565709</td><td>Averge Mn ~ 10,000</td></tr><tr><td>Poly(ethylene glycol)</td><td>Aldrich</td><td>373001</td><td>Average Mn 4,600</td></tr><tr><td>Biopsy punch</td><td>Miltex</td><td>33-36</td><td>Diameter = 6 mm</td></tr><tr><td>Aron Alpha</td><td>Toagosei Co., Ltd.</td><td></td><td>Instant glue</td></tr><tr><td>Universal testing machine (UTM)</td><td>Instron</td><td>5583</td><td></td></tr><tr><td>Microcentrifuge tubes</td><td>SPL life science</td><td>60015</td><td>1.5 ml</td></tr><tr><td>Petri dish</td><td>SPL life science</td><td>10090</td><td>90 x 15 mm</td></tr><tr><td>Sodium phosphate monobasic</td><td>Sigma</td><td>S5011</td><td>1x PBS ingredient</td></tr><tr><td>Sodium phosphate dibasic</td><td>Sigma</td><td>S5136</td><td>1x PBS ingredient</td></tr><tr><td>Sodium chloride</td><td>Duchefa biochemie</td><td>S0520.5000</td><td>1x PBS ingredient</td></tr><tr><td>Incubating shaker</td><td>Lab companion</td><td>SIF6000R</td><td></td></tr><tr><td>ICR mice</td><td>Orient bio</td><td>Normal ICR mouse</td><td>6 weeks, 30 - 35 g, male</td></tr><tr><td>Tiletamine-zolazepam (Zoletil 50)</td><td>Virbac</td><td></td><td></td></tr><tr><td>Zylazine (Rompun)</td><td>Bayer</td><td></td><td></td></tr><tr><td>PrecisionGlide&lt;sup&gt;TM&lt;/sup&gt; needle (18 G)</td><td>BD</td><td>302032</td><td>18 G</td></tr><tr><td>Filter paper</td><td>Whatman</td><td>1001 125</td><td>Diameter = 125 mm</td></tr><tr><td>Parafilm</td><td>Bemis Flexible Pakaging</td><td>PM996</td><td></td></tr></tbody></table>

tape: a biodegradable hemostatic glue inspired by a ubiquitous compound in plants for surgical application

이 동영상은 상업 조직 접착제보다 효과적인 지혈 능력과 큰 방수 접착 강도가 생분해 성 수술 용 접착제를 제조하기위한 간단한 프로토콜을 설명합니다. 의료 접착제로 인해 최소한의 침입과 사용에서의 편리 성을 봉합하고 스테이플에 대한 잠재적 인 대안 도구로 큰 관심을 받고있다. 생체 기반 물질의 경우와 같은 피브린 접착제 및 시아 노 아크릴 레이트 계 재료 등의 시판 사람들은 대부분들은 유기 분자 나 복잡한 단백질 정제 방법의 화학 합성의 일련의 요구를 포함한 조직 접착제를 개발하기위한 몇 가지 프로토콜이 있지만 (즉, 피브린 접착제). 또한, 외과 용 접착제의 개발이 생분해 성을 유지하기 때문에 인체의 습윤 환경에서 양호한 성능을 달성하는데 어려움이 여전히 도전하면서 높은 접착 성을 나타내는. 우리는을 준비하는 새로운 방법을 설명식물 유래 습식 강한 접착 분자의 물리적 혼합 한 후, 형성 물 혼화 초분자 집합체의 중량을 기반으로 분리하여, TAPE라고도 의료 접착제, T는 CID (TA), 및 잘 알려진 annic 생체 고분자, 폴리 (에틸렌) 글리콜 (PEG). 우리의 접근 방법으로, TAPE는 물의 존재 상용 피브린 접착제보다 2.5 배 높은 접착 강도를 나타낸다. 또한, TAPE는 생리 학적 조건에서 생분해 성 및 조직의 출혈에 대하여 강력한 지혈 접착제로서 사용될 수있다. 우리는 뮤코 접착 중합체, 약물 저장소 등과 같은 의료 설정 및 약물 전달 다양한 애플리케이션에서 테이프의 광범위한 사용을 예상한다.

1 부피비 (도 1A) TAPE는 TA (1 ㎍ / ml의 증류수)과 2 PEG (1 ㎍ / ml의 증류수)을 포함하는 두 수용액의 혼합물을 원심 분리 한 후 정착 초분자 집합체이다. 혼합 비율이 높은 접착 강도를 달성하는 데 중요한 요소이다 테이프 2로 형성하는 경우 : 1의 혼합비, TA, 25 단위의 수산기 (-OH)의 20 개는 최대 높은 분자간 수소 결합 형성의 결과로, PEG의 각 에테르 기 (-O-)와 상호 ...

Watch this Scientific Journal Video about TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application at JoVE.com

TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application

우리는 효과적인 지혈 기능을 갖는 의료용 생분해 성 접착제를 제조하는 가장 간단한 프로토콜을 설명한다. 테이프 탄닌산 혼합 한 물 - 비혼 화성 초분자 집합체 인 상업용 피브린 접착제에 비해 2.5 배 더 내수성 접착 성을 수득하는 유비쿼터스 화합물은 식물에서 발견 한 폴리 (에틸렌) 글리콜.

TAPE : 외과 응용 프로그램에 대한 식물의 유비쿼터스 화합물에서 영감을 생분해 성 지혈 접착제

This video describes the simplest protocol for preparing biodegradable surgical glue that has an effective hemostatic ability and greater water-resistant ...

tape-biodegradable-hemostatic-glue-inspired-ubiquitous-compound

Universidad de Cartagena UDEC

Research

JoVE Journal

Bioengineering

14.1K 조회수.  Korea Advanced Institute of Science and Technology (KAIST).  우리는 효과적인 지혈 기능을 갖는 의료용 생분해 성 접착제를 제조하는 가장 간단한 프로토콜을 설명한다. 테이프 탄닌산 혼합 한 물 - 비혼 화성 초분자 집합체 인 상업용 피브린 접착제에 비해 2.5 배 더 내수성 접착 성을 수득하는 유비쿼터스 화합물은 식물에서 발견 한 폴리 (에틸렌) 글리콜. 

비디오: TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological environment, both mechanical and chemical stimulation are required to maintain the proper development and functionality of arterial vessels1,2.
Bioreactor culture systems developed by our group are designed to support vessel regeneration within a precisely controlled chemo-mechanical environment mimicking that of native vessels. Our bioreactor assembly and maintenance procedures are fairly simple and highly repeatable3,4. Smooth muscle cells (SMCs) are seeded onto a tubular polyglycolic acid (PGA) mesh that is threaded over compliant silicone tubing and cultured in the bioreactor with or without pulsatile stimulation for up to 12 weeks. There are four main attributes that distinguish our bioreactor from some predecessors. 1) Unlike other culture systems that simulate only the biochemical surrounding of native blood vessels, our bioreactor also creates a physiological pulsatile environment by applying cyclic radial strain to the vessels in culture. 2) Multiple engineered vessels can be cultured simultaneously under different mechanical conditions within a controlled chemical environment. 3) The bioreactor allows a mono layer of endothelial cells (EC) to be easily coated onto the luminal side of engineered vessels for animal implantation models. 4) Our bioreactor can also culture engineered vessels with different diameter size ranged from 1 mm to 3 mm, saving the effort to tailor each individual bioreactor to fit a specific diameter size. 
The engineered vessels cultured in our bioreactor resemble native blood vessels histologically to some degree. Cells in the vessel walls express mature SMC contractile markers such as smooth muscle myosin heavy chain (SMMHC)3. A substantial amount of collagen is deposited within the extracellular matrix, which is responsible for ultimate mechanical strength of the engineered vessels5. Biochemical analysis also indicates that collagen content of engineered vessels is comparable to that of native arteries6. Importantly, the pulsatile bioreactor has consistently regenerated vessels that exhibit mechanical properties that permit successful implantation experiments in animal models3,7. Additionally, this bioreactor can be further modified to allow real-time assessment and tracking of collagen remodeling over time, non-invasively, using a non-linear optical microscopy (NLOM)8. To conclude, this bioreactor should serve as an excellent platform to study the fundamental mechanisms that regulate the regeneration of functional small-diameter vascular grafts.

Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.

타악기 생물 반응기를 사용하여 생물 공학 기반 혈관의 이식

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological ...

연구

생체공학

A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration

Sutures are a 4,000 year old technology that remain the 'gold-standard' for wound closure by virtue of their repair strength (~100 KPa). However, sutures can act as a nidus for infection and in many procedures are unable to effect wound repair or interfere with functional tissue regeneration.1 Surgical glues and adhesives, such as those based on fibrin and cyanoacrylates, have been developed as alternatives to sutures for the repair of such wounds. However, current commercial adhesives also have significant disadvantages, ranging from viral and prion transfer and a lack of repair strength as with the fibrin glues, to tissue toxicity and a lack of biocompatibility for the cyanoacrylate based adhesives. Furthermore, currently available surgical adhesives tend to be gel-based and can have extended curing times which limit their application.2 Similarly, the use of UV lasers to facilitate cross-linking mechanisms in protein-based or albumin 'solders' can lead to DNA damage while laser tissue welding (LTW) predisposes thermal damage to tissues.3 Despite their disadvantages, adhesives and LTW have captured approximately 30% of the wound closure market reported to be in excess of US $5 billion per annum, a significant testament to the need for sutureless technology.4
In the pursuit of sutureless technology we have utilized chitosan as a biomaterial for the development of a flexible, thin film, laser-activated surgical adhesive termed 'SurgiLux'. This novel bioadhesive uses a unique combination of biomaterials and photonics that are FDA approved and successfully used in a variety of biomedical applications and products. SurgiLux overcomes all the disadvantages associated with sutures and current surgical adhesives (see Table 1).
In this presentation we report the relatively simple protocol for the fabrication of SurgiLux and demonstrate its laser activation and tissue weld strength. SurgiLux films adhere to collagenous tissue without chemical modification such as cross-linking and through irradiation using a comparatively low-powered (120 mW) infrared laser instead of UV light. Chitosan films have a natural but weak adhesive attraction to collagen (~3 KPa), laser activation of the chitosan based SurgiLux films emphasizes the strength of this adhesion through polymer chain interactions as a consequence of transient thermal expansion.5 Without this 'activation' process, SurgiLux films are readily removed.6-9 SurgiLux has been tested both in vitro and in vivo on a variety of tissues including nerve, intestine, dura mater and cornea. In all cases it demonstrated good biocompatibility and negligible thermal damage as a consequence of irradiation.6-10

The fabrication of a novel, flexible thin film surgical adhesive from FDA approved ingredients, chitosan and indocyanine green is described. Bonding of this adhesive to collagenous tissue through a simple activation process with a low-powered infra-red laser is demonstrated.

키토산 기반, 레이저 활성화 박막 외과 접착제, &#39;SurgiLux&#39;: 준비 및 데모

Sutures are a 4,000 year old technology that remain the 'gold-standard' for wound closure by virtue of their repair strength (~100 KPa). However, sutures ...

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Natural polymers over the years have gained more importance because of their host biocompatibility and ability to interact with cells in vitro and in vivo. An area of research that holds promise in regenerative medicine is the combinatorial use of novel biomaterials and stem cells. A fundamental strategy in the field of tissue engineering is the use of three-dimensional scaffold (e.g., decellularized extracellular matrix, hydrogels, micro/nano particles) for directing cell function. This technology has evolved from the discovery that cells need a substrate upon which they can adhere, proliferate, and express their differentiated cellular phenotype and function 2-3. More recently, it has also been determined that cells not only use these substrates for adherence, but also interact and take cues from the matrix substrate (e.g., extracellular matrix, ECM)4. Therefore, the cells and scaffolds have a reciprocal connection that serves to control tissue development, organization, and ultimate function. Adipose-derived stem cells (ASCs) are mesenchymal, non-hematopoetic stem cells present in adipose tissue that can exhibit multi-lineage differentiation and serve as a readily available source of cells (i.e. pre-vascular endothelia and pericytes). Our hypothesis is that adipose-derived stem cells can be directed toward differing phenotypes simultaneously by simply co-culturing them in bilayered matrices1. Our laboratory is focused on dermal wound healing. To this end, we created a single composite matrix from the natural biomaterials, fibrin, collagen, and chitosan that can mimic the characteristics and functions of a dermal-specific wound healing ECM environment.

This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells1.

ASC의 분화를 제어하는​​ Bilayered 히드로겔 꾸민

Natural polymers over the years have gained more importance because of their host biocompatibility and ability to interact with cells in vitro and in ...

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two tissue edges1,2. These are then irradiated by a laser that is selectively absorbed by the RB. The resulting photochemical reactions supposedly crosslink the collagen fibers in the tissue with minimal heat production3. In this report, RB has been incorporated in thin chitosan films to fabricate a novel tissue adhesive that is laser-activated. Adhesive films, based on chitosan and containing ~0.1 wt% RB, are fabricated and bonded to calf intestine and rat tibial nerves by a solid state laser (&lambda;=532 nm, Fluence~110 J/cm2, spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, is used to test the bonding strength. The RB-chitosan adhesive bonds firmly to the intestine with a strength of 15 &plusmn; 6 kPa, (n=30). The adhesion strength drops to 2 &plusmn; 2 kPa (n=30) when the laser is not applied to the adhesive. The anastomosis of tibial nerves can be also completed without the use of sutures. A novel chitosan adhesive has been fabricated that bonds photochemically to tissue and does not require sutures.

Sutures are usually needed to repair tissue during surgical procedures. However, their application can be problematic as they are invasive and may damage tissue. The fabrication and application methods of a novel tissue adhesive are here reported. This adhesive film is laser-activated and does not require the use of sutures.

제조 및 레이저 조직 복구에 장미 벵골 - 키토산 영화의 응용

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two ...

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

Human induced pluripotent stem cells (hiPSCs) must be fully differentiated into specific cell types before administration, but conventional protocols for differentiating hiPSCs into cardiomyocytes (hiPSC-CMs), endothelial cells (hiPSC-ECs), and smooth muscle cells (SMCs) are often limited by low yield, purity, and/or poor phenotypic stability. Here, we present novel protocols for generating hiPSC-CMs, -ECs, and -SMCs that are substantially more efficient than conventional methods, as well as a method for combining cell injection with a cytokine-containing patch created over the site of administration. The patch improves both the retention of the injected cells, by sealing the needle track to prevent the cells from being squeezed out of the myocardium, and cell survival, by releasing insulin-like growth factor (IGF) over an extended period. In a swine model of myocardial ischemia-reperfusion injury, the rate of engraftment was more than two-fold greater when the cells were administered with the cytokine-containing patch comparing to the cells without patch, and treatment with both the cells and the patch, but not with the cells alone, was associated with significant improvements in cardiac function and infarct size.

We present three novel and more efficient protocols for differentiating human induced pluripotent stem cells into cardiomyocytes, endothelial cells, and smooth muscle cells and a delivery method that improves the engraftment of transplanted cells by combining cell injection with patch-mediated cytokine delivery.

다 능성 줄기 세포는 심근 수리를위한 심장 세포를 유도

Human induced pluripotent stem cells (hiPSCs) must be fully differentiated into specific cell types before administration, but conventional protocols for ...

발생학

Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial

Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral surgery. Unlike platelet rich plasma (PRP) that is a gel or a suspension, Leukocyte-Platelet Rich Fibrin (L-PRF) is a solid 3D fibrin membrane generated chair-side from whole blood containing no anti-coagulant. The membrane has a dense three dimensional fibrin matrix with enriched platelets and abundant growth factors. L-PRF is a popular adjunct in surgeries because of its superior handling characteristics as well as its suturability to the wound bed. The goal of the study is to demonstrate generation as well as provide detailed characterization of relevant properties of L-PRF that underlie its clinical success.

Leucocyte-Platelet Rich Fibrin (L-PRF) represents an FDA cleared preparation of autologous platelet concentrates that possesses unique fibrin architecture, enriched platelets and abundant growth factors. Here, we present a protocol for chair-side generation of L-PRF as well as evaluate its mechanical properties including uniaxial testing and suture retention strength testing.

백혈구 - 혈소판 풍부한 섬유소, 소설 생체 재료의 특성

Autologous platelet concentrates represent promising innovative tools in the field of regenerative medicine and have been extensively used in oral ...

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

During reconstructive bone surgeries, supraphysiological amounts of growth factors are empirically loaded onto scaffolds to promote successful bone fusion. Large doses of highly potent biological agents are required due to growth factor instability as a result of rapid enzymatic degradation as well as carrier inefficiencies in localizing sufficient amounts of growth factor at implant sites. Hence, strategies that prolong the stability of growth factors such as BMP-2/NELL-1, and control their release could actually lower their efficacious dose and thus reduce the need for larger doses during future bone regeneration surgeries. This in turn will reduce side effects and growth factor costs. Self-assembled PECs have been fabricated to provide better control of BMP-2/NELL-1 delivery via heparin binding and further potentiate growth factor bioactivity by enhancing in vivo stability. Here we illustrate the simplicity of PEC fabrication which aids in the delivery of a variety of growth factors during reconstructive bone surgeries.

Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.

헤파린 결합 도메인 골 형성 성장 인자 전달을위한 고분자 전해질 복합

During reconstructive bone surgeries, supraphysiological amounts of growth factors are empirically loaded onto scaffolds to promote successful bone ...

A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.

The murine MI model is widely recognized in the field of cardiovascular disease, and has consistently been used as a first step to test the efficacy of treatments in vivo1. The traditional, established protocol has been further fine-tuned to minimize the damage to the animal. Notably, the pectoral muscle layers are teased away rather than simply cut, and the thoracotomy is approached intercostally as opposed to breaking the ribs in a sternotomy, preserving the integrity of the ribcage. With these changes, the overall stress on the animal is decreased. 
Stem cell therapies aimed to alleviate the damage caused by MIs have shown promise over the years for their pro-angiogenic and anti-apoptotic benefits. Current approaches of delivering cells to the heart surface typically involve the injection of the cells either near the damaged site, within a coronary artery, or into the peripheral blood stream2-4. While the cells have proven to home to the damaged myocardium, functionality is limited by their poor engraftment at the site of injury, resulting in diffusion into the blood stream5. This manuscript highlights a procedure that overcomes this obstacle with the use of a cell-encapsulated hydrogel patch. The patch is fabricated prior to the surgical procedure and is placed on the injured myocardium immediately following the occlusion of the left coronary artery. To adhere the patch in place, biocompatible external fibrin glue is placed directly on top of the patch, allowing for it to dry to both the patch and the heart surface. This approach provides a novel adhesion method for the application of a delicate cell-encapsulating therapeutic construct.

This protocol aims to alleviate the limitation of poor cell engraftment for stem cell treatment of myocardial infarctions through the use of a hydrogel system and a fibrin-based glue. With this approach, cell-to-tissue contact post-infarction can be maintained, increasing the therapeutic potential of beneficial agents at the site of injury.

하이드로 겔은 구축 및 쥐 심근 경색 모델에서 치료를 제공하기 위해 접착제 접근을 섬유소 기반.

The murine MI model is widely recognized in the field of cardiovascular disease, and has consistently been used as a first step to test the efficacy of ...

의학

Synthesis of Strong Adhesive Hydrogel, Gelatin O-Nitrosobenzaldehyde

Adhesive materials have become popular biomaterials in the field of biomedical and tissue engineering. In our previous work, we presented a new material - gelatin o-nitrosobenzaldehyde (gelatin-NB) - which is mainly used for tissue regeneration and has been validated in animal models of corneal injury and inflammatory bowel disease. This is a novel hydrogel formed by modifying biological gelatin with o-nitrosobenzaldehyde (NB). Gelatin-NB was synthesized by activating the carboxyl group of NB-COOH and reacting with gelatin through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). The obtained compound was purified to generate the final product, which can be stably stored for at least 18 months. NB has a strong adhesion to -NH2 on the tissue, which can form many C = N bonds, thus increasing the adhesion of gelatin-NB to the tissue interface. The preparation process comprises steps for the synthesis of the NB-COOH group, modification of the group, synthesis of gelatin-NB, and purification of the compound. The goal is to describe the specific synthesis process of gelatin-NB in detail and to demonstrate the application of gelatin-NB to damage repair. Moreover, the protocol is presented to further strengthen and expand the nature of the material produced by the scientific community for more applicable scenarios.

The protocol presented here shows the synthesis of a strong adhesive hydrogel gelatin o-nitrosobenzaldehyde (gelatin-NB). Gelatin-NB has rapid and efficient tissue adhesion ability, which can form a strong physical barrier to protect wound surfaces, so it is expected to be applied to the field of injury repair biotechnology.

강력한 접착력 하이드로겔, 젤라틴 O-니트로소벤즈알데히드의 합성

Adhesive materials have become popular biomaterials in the field of biomedical and tissue engineering. In our previous work, we presented a new material - ...

Occlusion of the Great and Small Saphenous Vein Using Copolymeric Glue Based on N-Butyl Cyanoacrylate and Methacryloxy Sulfolane

We present the preliminary results of a longitudinal observational study aimed at evaluating the effectiveness and safety at different short- and long-term follow-ups of vascular occlusion of the great saphenous vein (GSV) and small saphenous vein (SSV) affected by severe pathological reflux, using an innovative modified cyanoacrylate surgical glue composed of N-butyl cyanoacrylate and methacryloxy sulfolane (NBCA+MS). Ninety patients, prospectively recruited for 1 year, underwent the study with EcoColor-Doppler (ECD) to evaluate the maximum diameters of the GSV and SSV in the orthostatic position and the reflux time (RT). An RT greater than 0.5 s was considered pathologic. Clinical, etiology, anatomy, and pathophysiology (CEAP) assessment was used for the complete evaluation of each patient in the study. All the patients were treated by NBCA+MS glue to obtain vein occlusion and observed before treatment (baseline; T0), within 6 h after treatment (T1), 1 month after treatment (T2), 3 months after treatment (T3), 6 months after treatment (T4), and 1 year after treatment (T5). Chi-square (&#967;) analysis was performed to evaluate the effectiveness and safety of the treatment. All the patients participated in the entire duration of the study. Complete occlusion was maintained in 100% of patients at T1, 98.9% at T2 and T3, and 97.8% at T4 and T5 (p &lt; 0.001). None of the patients suffered from post-surgical thrombosis. No blue hyperpigmentation, or paresthesia was observed during the entire observation period. Immediately after treatment, 7.7% of patients needed painkillers; 1 week after treatment, 100% of patients returned to normal life. Vascular occlusion of the great or small saphenous vein using NBCA+MS glue is a safe procedure with persistent benefits after a 1 year follow-up. This procedure can be performed with local anesthesia, allowing a quick return to normal life. Thanks to its low invasiveness, the treatment is not painful.

Here, we present a protocol to treat the great saphenous vein (GSV) and the small saphenous vein (SSV) affected by severe pathological reflux, using an original sclerosing and embolizing cyanoacrylate-based glue composed of N-butyl cyanoacrylate and methacryloxy sulfolane (NBCA+MS).

TAPE : 외과 응용 프로그램에 대한 식물의 유비쿼터스 화합물에서 영감을 생분해 성 지혈 접착제

요약

더 많은 비디오 탐색

이 비디오의 챕터

타악기 생물 반응기를 사용하여 생물 공학 기반 혈관의 이식

키토산 기반, 레이저 활성화 박막 외과 접착제, 'SurgiLux': 준비 및 데모

ASC의 분화를 제어하는 Bilayered 히드로겔 꾸민

제조 및 레이저 조직 복구에 장미 벵골 - 키토산 영화의 응용

다 능성 줄기 세포는 심근 수리를위한 심장 세포를 유도

백혈구 - 혈소판 풍부한 섬유소, 소설 생체 재료의 특성

헤파린 결합 도메인 골 형성 성장 인자 전달을위한 고분자 전해질 복합

하이드로 겔은 구축 및 쥐 심근 경색 모델에서 치료를 제공하기 위해 접착제 접근을 섬유소 기반.

강력한 접착력 하이드로겔, 젤라틴 O-니트로소벤즈알데히드의 합성

N- 부틸 시아 노 아크릴 레이트와 메타 크릴 옥시 설폴란을 기반으로 한 공중 접착제를 사용한 크고 작은 복재 정맥의 폐색

TAPE : 외과 응용 프로그램에 대한 식물의 유비쿼터스 화합물에서 영감을 생분해 성 지혈 접착제

요약

더 많은 비디오 탐색

이 비디오의 챕터

타악기 생물 반응기를 사용하여 생물 공학 기반 혈관의 이식

키토산 기반, 레이저 활성화 박막 외과 접착제, 'SurgiLux': 준비 및 데모

ASC의 분화를 제어하는​​ Bilayered 히드로겔 꾸민

제조 및 레이저 조직 복구에 장미 벵골 - 키토산 영화의 응용

다 능성 줄기 세포는 심근 수리를위한 심장 세포를 유도

백혈구 - 혈소판 풍부한 섬유소, 소설 생체 재료의 특성

헤파린 결합 도메인 골 형성 성장 인자 전달을위한 고분자 전해질 복합

하이드로 겔은 구축 및 쥐 심근 경색 모델에서 치료를 제공하기 위해 접착제 접근을 섬유소 기반.

강력한 접착력 하이드로겔, 젤라틴 O-니트로소벤즈알데히드의 합성

N- 부틸 시아 노 아크릴 레이트와 메타 크릴 옥시 설폴란을 기반으로 한 공중 접착제를 사용한 크고 작은 복재 정맥의 폐색

ASC의 분화를 제어하는 Bilayered 히드로겔 꾸민