JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

Journal - immunology and infection

Reprograming Model of Human Monocyte-derived Macrophages for <em>In-vitro</em> Assays

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

115 views

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2025

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Eugenio Antonio Carrera Silva¹, Andrea Emilse Errasti²

¹Instituto de Medicina Experimental (IMEX), Academia Nacional de Medicina-CONICET, ²Instituto de Farmacología, Facultad de Medicina, Universidad de Buenos Aires

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level

549 views

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2025

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Zelvera Ismetova Usheva¹, David Leander Petersen², Torben Barington¹, Peter Mouritzen², Mike Bogetofte Barnkob¹

¹Centre for Cellular Immunotherapy of Haematological Cancer Odense (CITCO), Department of Clinical Immunology, Odense University Hospital, ²Samplix ApS

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

406 views

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2025

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Anjali S. Yennemadi¹, Joseph Keane¹, Gina Leisching¹

¹TB Immunology Group, Department of Clinical Medicine, Trinity Translational Medicine Institute, St James's Hospital, Trinity College Dublin, The University of Dublin

Prion Safety Laboratory Swipe Test

Prion Safety Laboratory Swipe Test

461 views

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2025

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Sara M. Simmons¹, Qi Yuan¹, Jason C. Bartz¹

¹Department of Medical Microbiology and Immunology, School of Medicine, Creighton University

Fluorescence Assays for the Study of <em>Mycobacterium tuberculosis</em> Interaction with the Immune Receptor SLAMF1

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

254 views

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2025

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Angela María Barbero^1,2, Rodrigo Emanuel Hernández Del Pino^1,2, Verónica Edith García^3,4, Virginia Pasquinelli^1,2

¹Centro de Investigaciones Básicas y Aplicadas (CIBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), ²Centro de Investigaciones y Transferencias del Noroeste de la Provincia de Buenos Aires (CIT NOBA), UNNOBA- Universidad Nacional de San Antonio de Areco (UNSAdA)- Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ³CONICET- Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), ⁴Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Universidad de Buenos Aires

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology

Optimization of Antigen Preparation for Hemagglutination Inhibition Assay of Newcastle Disease Virus Serology

276 views

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2025

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Chaoyang Chen^1,2, Huajun Yang^1,2, Xiaoxia Ji³, Cheng Li¹, Mengting Yang¹, Lin Ge¹, Mengting Sun¹

¹Department of Medical Science and Technology, Suzhou Chien-shiung Institute of Technology, ²Jiangsu Province Engineering Research Center of Novel Tumor-targeting Drug Conjugates, ³Crown Bioscience Inc.

Preparation of <i>Mycobacterium tuberculosis</i> Culture Filtrate to Understand TB Pathogenesis

Preparation of Mycobacterium tuberculosis Culture Filtrate to Understand TB Pathogenesis

252 views

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2025

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Priyadarshini Sanyal^1,2, Vinay Kumar Nandicoori^1,2,3

¹CSIR-Centre for Cellular and Molecular Biology (CSIR-CCMB), ²Academy of Scientific and Innovative Research (AcSIR), ³National Institute of Immunology

Development and Assessment of Intracellular Infection Models for <i>Staphylococcus aureus</i>

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

339 views

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2025

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Chengwen Zhang^1,2, Chuanfei Xu², Fumei Luo^2,3, Yu Zuo², Ping Luo², Ping Cheng², Weijun Zhang², Si Sun², Hao Zeng², Quanming Zou²

¹Medical Research Institute, Southwest University, ²National Engineering Research Center of Immunological Products, Third Military Medical University, ³School of Pharmacy, University of South China

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

456 views

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2025

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Katherine Do¹, Subina Mehta¹, Reid Wagner², Timothy J. Griffin¹, Pratik D. Jagtap¹

¹Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, ²Minnesota Supercomputing Institute, University of Minnesota

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

235 views

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2024

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Kathryn M. Crotty^1,2, Samantha M. Yeligar^1,2

¹Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Emory University, ²Atlanta Veterans Affairs Health Care System

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