この研究は、中国国立自然科学財団(No. 81922076, 81973951, 81873373), 上海の共同イノベーションの建設プロジェクトによって支援されました (No.ZYJKFW201811010、ザイクFW201701005)。

すべての動物実験は、上海伝統中国医学大学動物実験倫理委員会(倫理クリアランス番号PZSHUTCM190308020)によって審査され、承認されました。
1. ハイプラットフォームの製作
<ol><li>サポートデバイスの長さと幅を86 cm x 17 cmに設定します(図1)。</li>
	<li>接続デバイスをサポートデバイスに垂直に固定します。接続デバイスは、ラットが高いプラットフォームの上面に走るのを防ぐために約30cmの長さであり、幅は14cmである必要があります。</li>
	<li>接続デバイスに水平ペダル(5cm x 5 cm)を固定します。水平ペダルの底部の吊り下げ高さは50cm以上でなければなりません。 注:実験ラットの数に応じて、複数の水平ペダルを使用することができ、各ペダルに1匹のラットを配置します。</li>
</ol>2. 喘息のラットモデルの確立
<ol><li>OVAでラットを感作する<ol>
<li>0日目に感作液を準備します。1 mgのOVAと10mgの水酸化アルミニウムを溶媒として、それぞれ1mLの感作液を調製する。</li>
		<li>腹腔内に、0日目に1mLの感作液を各ラットに注入する。後で使用するために適当な注射器で感作液を描きます。</li>
		<li>尾をつかんでラットを持ち上げ、粗い表面に置き、尾をそっと引き戻します。左手親指と人差し指で両耳と首の皮膚をつかみ、残りの3本の指と手のひらで背中の皮膚をしっかりと握りしめる(ネズミを窒息させる余分な力を加えないように注意してください)。</li>
		<li>右手で、1mLの感作液で調製した注射器を用いてラットに腹腔内注射を行う。</li>
	</ol>
</li>
	<li>OVAを使用して挑戦ラット<ol>
<li>ふた付きの箱(長さ、幅、高さは39cm x 28cm x 23cm)を選択し、側面に小さな穴を開けます。密閉ボックス、ネビュライズカップ、圧縮ネブライザーをゴムチューブで接続します(補助図1)。 注:圧縮ネブライザーのネビュライズされた粒子サイズは1〜5μm、ネビュレーション速度は0.2 mL/minより大きく、最終的な圧力は205 kPaです。</li>
		<li>感作後14日目、自家製密封箱の蓋を開け、箱にネズミを入れ(箱は5匹のラットを収容できる)、密封箱をバイオセーフティキャビネットに入れます。</li>
		<li>チャレンジ ソリューションを準備します。完全に正常な生理液の30 mLとOVAの300mgを混合します。</li>
		<li>超音波ネブライザーをオンにし、ネビュライズカップに挑戦溶液の30 mLを追加します。自家製の密封箱の体積に応じて、チャレンジ溶液の投与量とラット数を調整します。</li>
		<li>ネビュライズを数回(1日1回)繰り返すか、喘息症状の重症度に応じて手順を調整します。</li>
	</ol>
</li>
</ol>3. 鍼治療
<ol><li>喘息を治療するための伝統的な中国医学の理論に従って、GV14、両側BL12および両側BL13を含むアクポイントを選択する(補足図2)。2日目に手動鍼治療を行い、1日おきに1日に1回、合計7セッションずつ実行します(図2)。
	<ol>
<li>第7頸椎と第1胸椎(肩甲骨の中空)の間に位置するGV14を後ろの正中線に鍼治療する(図3)。</li>
		<li>肋間空間(肩甲骨の下端)の両側にある第3胸椎の下に位置するBL13を鍼治療する。</li>
		<li>第2胸椎下、肋間空間の両側(肩甲骨の下、BL13に従って特定された位置)の下に位置するBL12を鍼治療する。</li>
	</ol>
</li>
	<li>ラットをつかみ、高いプラットホームに置きます。</li>
	<li>使い捨てのステンレス製の針 (0.3mm x 13.0 mm)を右手の親指と人差し指でハンドルに持ち、右手の手のひらでラットの後ろを押します。 注: 使い捨て針は、1 回のみ使用してください。繰り返し使用すると針が鈍くなり、ラットの痛みが増加するからです。</li>
	<li>上端の中央(GV14)または肩甲骨の下端(BL12とBL13)のくぼみに触れ、ラットの皮膚を針先でそっと刺して、指差しを見つけます。</li>
	<li>指差しの特定の位置を決定した後、針を素早く挿入し、すぐに右手を取り外します。</li>
	<li>10~20秒間60回/分の割合で針を約360°均等にねじり、5分ごとに操作し、20~30分後に引き出した(補足図4)。</li>
</ol>4. ペンの測定
<ol><li>メタコリンを準備する: 5 mL 遠心分離チューブにメタコリンの 100 mg を配置し、2 mL (50 mg/mL メタコリン) のボリュームに PBS を追加します。その後、50 mg/mL メタコリン溶液を25 mg/mL、12.5 mg/mL、6.25 mg/mL、3.125 mg/mL に希釈します。</li>
	<li>機器検査<ol>
<li>信号増幅器をオンにします(エアロゾル分布システムとげっ歯類バイアスフロー供給システムはすべてオフになり、ネビュライズコネクタは閉じています)。</li>
		<li>チャンネルを確認する :[キャリブレーション]をクリックし、粗い調整ベースラインを0に戻し、直流(DC)位置に切り替えます。ベースラインを0に微調整し、5 mLのガスをシリンジで注入します。次に、交流(AC)位置に切り替えます。</li>
		<li>チャネルを確認した後、エラー値が 0.5% 未満であることを確認します。</li>
	</ol>
</li>
	<li>パラメータの設定<ol>
<li>エアロゾル分布システムとげっ歯類バイアスフロー供給システムをオンにします。水銀球の液面に応じて流量を4.5mLに調整します。</li>
		<li>[ スタディ オプションの作成 ] をクリックし、[ 線量応答スタディ] を選択し、[ 種のラット ] を選択して 、[ 次へ] をクリックします。</li>
		<li>[ 単純な線量リスト] を選択し、[ 線量の数 ] を 6 に設定して、[ 次へ] をクリックします。</li>
		<li>PenhとAreaパラメータを選択します: 動物の適応時間を 5 分、ネビュライズ時間を 2 分、応答時間を 3 分、回復時間を 2 分に設定します。</li>
		<li>ネブライザーに次の溶液を順次添加します(ラット当たり200 μL):メタコリン(0 mg/mL、3.125 mg/mL、6.25 mg/mL、12.5 mg/mL、25 mg/mLおよび50 mg/mL)。Penhのリアルタイム変化を観察することができます(図4)。</li>
		<li>濃度の異なる 6 つのソリューションがすべて反応した後、[ ファイル と セッションの終了 ] をクリックして実験を完了します。</li>
	</ol>
</li>
</ol>

<ol>
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著者らは開示するものは何もない。

この研究で使用されるGV14、両側BL12および両側BL13のacupointsは背に位置し、したがって、高いプラットホームはラットを固定するのに適している。他の研究では、適切な固定法は、指数15の位置に応じて選択する必要があります。腹部の指差しの場合、ラットは黒いコーン(パン屋が使用するペストリーバッグに似ています)17に下方に置くことができます。固?...

喘息は気管支収縮および気道過敏性1を特徴とする慢性疾患である。世界中で約3億3,940万人が喘息2と診断されている。喘息の罹患率は世界的に増加しており、今後10年間で1億人の新しい症例が予想される3 .吸入コルチコステロイドおよび長時間作用型β 2-アゴニストは、全患者の症状を効果的に制御しない4.したがって、喘息を治療するための鍼治療などの代替療法の使用は、注目度が高まっている5。喘息を治療するための有効な方法は、針GV14(ダズイ)、両側BL12(風門)および両側BL13(フェイシュ)6.鍼治療は、アレルギー性喘息患者の唾液および鼻分泌物中の分泌免疫グロブリンA(SIgA)のレベルを有意に低下させ、総有効率85%6で末梢血好酸球数を減少させる。慢性喘息を有するマウスにおけるBL13およびST36(Zusanli)における鍼治療は、IL-17のレベルを40%減少させ、オボアルブミン(OVA)群のそれと比較して平滑筋厚を33%減少させ、したがって症状7,8を緩和する。しかし、喘息に対する鍼治療のメカニズムは完全には明らかではない。
動物モデルは喘息治療10の有効性を評価する呼吸機能の連続測定に使用することができるので、9の喘息研究のための重要なツールである。一方、喘息の病態生理学の研究は、主要因子11のレベルの変化を検証するために気管、肺および気管支肺胞洗浄からのサンプルを含む試験サンプルを必要とする。喘息のラットモデルは、長期にわたる気道応答を産生し、即時および後期気道応答12を示すため、喘息病態生理を評価するために一般的に使用される。しかし、繰り返される実験的刺激のために、ラットはしばしば過敏になる。そのため、ラットを固定するための適切な方法が必要である。ラット固定のために一般的に使用される方法には、麻酔および結合13が含まれる。麻酔法は、視力の露出を改善するが、神経伝導に影響を与え、最終的には実験結果14に影響を与える可能性がある。この結合方法は麻酔と同じ生理学的効果を誘発しないが、抑制されたラットは骨格筋の緊張を経験する。また、針は指定位置15に容易に達することができない。ここでは、ラットを制限せずに固定し、背中の指を完全に露出させるために使用できる高いプラットフォームを紹介します。ラットは、鍼治療の投与中に高いプラットフォーム上に配置することができます。高いプラットホームはラットが立っている間だけ収容することができ、そして地面から一定の距離に置かれるので、ラットは高所16の恐怖のために動かないだろう。
この記事では、高いプラットフォームの製造、喘息のラットモデルの確立、高いプラットフォーム上でのラットの必要性の操作、全身プレチスモグラフィー(WBP)システムを用いた呼吸機能の測定について詳細に説明する。

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	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:267px;">0.3 mm x 13.0 mm acupuncture needle</td>
			<td style="width:192px;">China Suzhou Medical Device Factory</td>
			<td style="width:114px;"></td>
			<td style="width:154px;"></td>
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			<td height="25" style="height:24px;width:267px;">Al(OH)3</td>
			<td style="width:192px;">American Thermo</td>
			<td style="width:114px;">77161</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">Compression nebulizer</td>
			<td style="width:192px;">Jiangsu Lude Medical Electronics Co., Ltd.</td>
			<td style="width:114px;">NB-212C</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Fine Pointe V2.0</td>
			<td style="width:192px;">American Buxco</td>
			<td style="width:114px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Laboratory gas drying unit</td>
			<td style="width:192px;">American Drierite</td>
			<td style="width:114px;">26800</td>
			<td></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:267px;">Methacholine</td>
			<td style="width:192px;">American Sigma</td>
			<td style="width:114px;">MKCF6054</td>
			<td></td>
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			<td height="21" style="height:21px;width:267px;">Ovalbumin</td>
			<td style="width:192px;">American Sigma</td>
			<td style="width:114px;">A5503-25G</td>
			<td></td>
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			<td height="21" style="height:21px;width:267px;">Phosphate Buffer solution (1x)</td>
			<td style="width:192px;">Gelifesciences</td>
			<td style="width:114px;">SH30256.01</td>
			<td></td>
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			<td height="21" style="height:21px;width:267px;">Seal box</td>
			<td style="width:192px;">IRIS Corporation of Japan</td>
			<td style="width:114px;"></td>
			<td></td>
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			<td height="42" style="height:42px;width:267px;">Whole-body plethysmography respiratory function measurement system</td>
			<td style="width:192px;">American Buxco</td>
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			<td></td>
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	</tbody>
</table>

acupuncture in a rat model of asthma

高いプラットホームは制限なしでラットを固定し、鍼治療の操作の間に背の指を完全に露出させることができる。この記事では、高いプラットフォームの製造方法について説明し、喘息のラットモデルを確立し、非侵襲的かつリアルタイム全身プレチスモグラフィー(WBP)システムを使用して呼吸機能の変化を測定する。

GV14、両側BL12および両側BL13ポイントで鍼治療を行い、ラットは起こりやすい位置を維持する。一方向が木製の板で塞がれているため、ラットは頭を外側にだけねじることができ、内側にはねじれない(図5)。
メタコリン濃度が増加するにつれて, ペンは増加傾向を示し、成長速度は徐々に増加します..3.125 mg/mL、6.25 mg/mL、12.5 mg/mL、25 mg/mL、50 mg/mLの濃?...

Watch this Scientific Journal Video about Acupuncture in a Rat Model of Asthma at JoVE.com

Acupuncture in a Rat Model of Asthma

喘息ラットモデルにおける鍼治療

A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes ...

Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

5.9K ビュー.  Shanghai University of Traditional Chinese Medicine. 高いプラットホームは制限なしでラットを固定し、鍼治療の操作の間に背の指を完全に露出させることができる。この記事では、高いプラットフォームの製造方法について説明し、喘息のラットモデルを確立し、非侵襲的かつリアルタイム全身プレチスモグラフィー(WBP)システムを使用して呼吸機能の変化を測定する。

ビデオ: Acupuncture in a Rat Model of Asthma

Bronchial Thermoplasty:  A Novel Therapeutic Approach to Severe Asthma

Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks. Bronchial thermoplasty is delivered by the Alair System and is performed in three outpatient procedure visits, each scheduled approximately three weeks apart. The first procedure treats the airways of the right lower lobe, the second treats the airways of the left lower lobe and the third and final procedure treats the airways in both upper lobes. After all three procedures are performed the bronchial thermoplasty treatment is complete.
Bronchial thermoplasty is performed during bronchoscopy with the patient under moderate sedation. All accessible airways distal to the mainstem bronchi between 3 and 10 mm in diameter, with the exception of the right middle lobe, are treated under bronchoscopic visualization. Contiguous and non-overlapping activations of the device are used, moving from distal to proximal along the length of the airway, and systematically from airway to airway as described previously. Although conceptually straightforward, the actual execution of bronchial thermoplasty is quite intricate and procedural duration for the treatment of a single lobe is often substantially longer than encountered during routine bronchoscopy. As such, bronchial thermoplasty should be considered a complex interventional bronchoscopy and is intended for the experienced bronchoscopist. Optimal patient management is critical in any such complex and longer duration bronchoscopic procedure. This article discusses the importance of careful patient selection, patient preparation, patient management, procedure duration, postoperative care and follow-up to ensure that bronchial thermoplasty is performed safely.
Bronchial thermoplasty is expected to complement asthma maintenance medications by providing long-lasting asthma control and improving asthma-related quality of life of patients with severe asthma. In addition, bronchial thermoplasty has been demonstrated to reduce severe exacerbations (asthma attacks) emergency rooms visits for respiratory symptoms, and time lost from work, school and other daily activities due to asthma.

Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks.

気管支熱形成術：重篤な喘息の新しい治療アプローチ

Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled ...

医学

Angiogenesis in the Ischemic Rat Lung

The adult lung is perfused by both the systemic bronchial artery and the entire venous return flowing through the pulmonary arteries. In most lung pathologies, it is the smaller systemic vasculature that responds to a need for enhanced lung perfusion and shows robust neovascularization. Pulmonary vascular ischemia induced by pulmonary artery obstruction has been shown to result in rapid systemic arterial angiogenesis in man as well as in several animal models. Although the histologic assessment of the time course of bronchial artery proliferation in rats was carefully described by Weibel 1, mechanisms responsible for this organized growth of new vessels are not clear. We provide surgical details of inducing left pulmonary artery ischemia in the rat that leads to bronchial neovascularization. Quantification of the extent of angiogenesis presents an additional challenge due to the presence of the two vascular beds within the lung. Methods to determine functional angiogenesis based on labeled microsphere injections are provided.

The lung is perfused by both the systemic bronchial artery and pulmonary arteries. In most lung pathologies, it is the smaller systemic vasculature that shows robust neovascularization. Cessation of pulmonary blood flow promotes brisk bronchial angiogenesis. We provide surgical details of inducing left pulmonary artery ischemia that promotes bronchial neovascularization.

虚血ラットの肺の血管新生

The  adult lung is perfused by both the systemic bronchial artery and the entire  venous return flowing through the pulmonary arteries. In most lung ...

A Model of Chronic Nutrient Infusion in the Rat

Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessive levels of glucose and fatty acids affect the pancreatic beta-cell and the secretion of insulin, we have established a chronic nutrient infusion model in the rat. The procedure consists of catheterizing the right jugular vein and left carotid artery under general anesthesia; allowing a 7-day recuperation period; connecting the catheters to the pumps using a swivel and counterweight system that enables the animal to move freely in the cage; and infusing glucose and/or Intralipid (a soybean oil emulsion which generates a mixture of approximately 80% unsaturated/20% saturated fatty acids when infused with heparin) for 72 hr. This model offers several advantages, including the possibility to finely modulate the target levels of circulating glucose and fatty acids; the option to co-infuse pharmacological compounds; and the relatively short time frame as opposed to dietary models. It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context.

A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.

ラットにおける慢性栄養注入のモデル

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis

Pyelonephritis is a bacterial infection of the kidney and is most commonly caused by Escherichia coli. Recurrent infections can cause significant renal inflammation and fibrosis ultimately resulting in declining kidney function. Before improved clinical management and prevention of pyelonephritis can be instituted, a reliable animal model must be established in order to study the mechanisms of progression, recurrence, and therapeutic efficacy. The transurethral infection model closely mimics human pyelonephritis but exhibits considerable variation due to its reliance on urethral reflux to transport the bacteria to the kidney. Herein, a detailed surgical protocol for performing bacterial injections into the rat renal pelvis is provided and confirmed by non-invasive Magnetic Resonance Imaging (MRI). Using this protocol, animals receive direct exposure to a desired concentration of E. coli bacteria and can fully recover from the surgical procedure with adequate post-operative care. This facilitates subsequent longitudinal MRI assessments of the experimental animal models for comparison with saline (sham) controls. Using this direct delivery approach, the severity of infection is controllable and applicable for mechanistic studies of progression as well as development of novel treatment strategies.

Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis

This manuscript describes a rat surgical model of pyelonephritis using direct intra-renal infection by Escherichia coli into the renal pelvis. The experimental procedure can be utilized to study the pathogenesis of pyelonephritis as well as the associated inflammation and fibrosis.

腎内注射<em&gt;大腸菌</em&gt;腎盂腎炎のラットモデル

Pyelonephritis is a bacterial infection of the kidney and is most commonly caused by Escherichia coli. Recurrent infections can cause significant renal ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

ワイヤー図法を使用してマウスの腸間膜動脈の等尺性収縮力測定

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Retinal Pathophysiological Evaluation in a Rat Model

A posterior segment eye disease like diabetic retinopathy alters the physiology of the retina. Diabetic retinopathy is characterized by a retinal detachment, breakdown of the blood-retinal barrier (BRB), and retinal angiogenesis. An in vivo rat model is a valuable experimental tool to examine the changes in the structure and function of the retina. We propose three different experimental techniques in the rat model to identify morphological changes of retinal cells, retinal vasculature, and compromised BRB. Retinal histology is used to study the morphology of various retinal cells. Also, quantitative measurement is performed by retinal cell count and thickness measurement of different retinal layers. A BRB breakdown assay is used to determine the leakage of extraocular proteins from the plasma to vitreous tissue due to the breakdown of BRB. Fluorescence angiography is used to study angiogenesis and leakage of blood vessels by visualizing retinal vasculature using FITC-dextran dye.

Diabetic retinopathy is one of the leading causes of blindness. Histology, blood-retinal barrier breakdown assay, and fluorescence angiography are valuable techniques to understand the pathophysiology of the retina, which could further enhance the efficient drug screening against diabetic retinopathy.

ラットモデルにおける網膜病態生理学的評価

A posterior segment eye disease like diabetic retinopathy alters the physiology of the retina. Diabetic retinopathy is characterized by a retinal ...

A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts

Ventricular fibrillation (VF) is a fatal arrhythmia with a high incidence in cardiac patients, but VF arrest under perfusion is a neglected method of intraoperative arrest in the field of cardiac surgery. With recent advances in cardiac surgery, the demand for prolonged VF studies under perfusion has increased. However, the field lacks simple, reliable, and reproducible animal models of chronic ventricular fibrillation. This protocol induces long-term VF through alternating current (AC) electrical stimulation of the epicardium. Different conditions were used to induce VF, including continuous stimulation with a low or high voltage to induce long-term VF and stimulation for 5 min with a low or high voltage to induce spontaneous long-term VF. The success rates of the different conditions, as well as the rates of myocardial injury and recovery of cardiac function, were compared. The results showed that continuous low-voltage stimulation induced long-term VF and that 5 min of low-voltage stimulation induced spontaneous long-term VF with mild myocardial injury and a high rate of recovery of cardiac function. However, the low-voltage, continuously stimulated long-term VF model had a higher success rate. High-voltage stimulation provided a higher rate of VF induction but showed a low defibrillation success rate, poor recovery of cardiac function, and severe myocardial injury. On the basis of these results, continuous low-voltage epicardial AC stimulation is recommended for its high success rate, stability, reliability, reproducibility, low impact on cardiac function, and mild myocardial injury.

This protocol presents a model of long-term ventricular fibrillation in rat hearts induced by continuous stimulation with low-voltage alternating current. This model has a high success rate, is stable, reliable, and reproducible, has a low impact on cardiac function, and causes only mild myocardial injury.

単離ラット心臓における長期心室細動のモデル

Ventricular fibrillation (VF) is a fatal arrhythmia with a high incidence in cardiac patients, but VF arrest under perfusion is a neglected method of ...

ラットモデルにおけるFSN療法の改善:不快感の最小化と効率の最大化

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the mechanisms of action are still not fully understood. During FSN treatment, the FSN needle is inserted and retained in the subcutaneous tissues for a long duration with a swaying movement. However, challenges arise from maintaining a posture while manipulating FSN in animal models (e.g., rats) for researchers. Uncomfortable treatment can lead to fear and resistance to FSN needles, increasing the risk of injury and may even affect research data. Anesthesia may also affect the study results too. Hence, there is a need for strategies in FSN therapy on animals that minimize injury during the intervention. This study employs a chronic constriction injury model in Sprague-Dawley rats to induce neuropathic pain. This model replicates the pain induced by nerve injury observed in humans through surgical constriction of a peripheral nerve, mimicking the compression or entrapment seen in conditions such as nerve compression syndromes and peripheral neuropathies. We introduce an appropriate manipulation for easily inserting an FSN needle into the subcutaneous layer of the animal&#39;s body, including needle insertion and direction, needle retention, and swaying movement. Minimizing the rat&#39;s discomfort prevents the rat from being tense, which causes the muscle to contract and hinder the entry of the needle and improves the study efficiency.

著者スポットライト:FSN治療の治療効果を明らかにする – 神経障害性疼痛における研究と臨床応用の橋渡し

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.

坐骨神経痛に対するフー皮下ニードリングの有効性:慢性狭窄損傷ラットモデルにおける行動的および電気生理学的変化

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the ...

喘息ラットモデルにおける鍼治療

要約

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この動画の章

気管支熱形成術：重篤な喘息の新しい治療アプローチ

虚血ラットの肺の血管新生

ラットにおける慢性栄養注入のモデル

腎内注射

ワイヤー図法を使用してマウスの腸間膜動脈の等尺性収縮力測定

ラットモデルにおける網膜病態生理学的評価

単離ラット心臓における長期心室細動のモデル

坐骨神経痛に対するフー皮下ニードリングの有効性:慢性狭窄損傷ラットモデルにおける行動的および電気生理学的変化

坐骨神経痛に対するフー皮下ニードリングの有効性:慢性狭窄損傷ラットモデルにおける行動的および電気生理学的変化

坐骨神経痛に対するフー皮下ニードリングの有効性:慢性狭窄損傷ラットモデルにおける行動的および電気生理学的変化